Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk

Lactic acid bacteria is well known for it uses as starter culture in various fermented food, and it functions as a good natural antimicrobial agent. Cincaluk, a Malaysian fermented shrimp product commonly found in traditional dishes is commonly enriched with LAB. Out of 50 colonies from a local cinc...

Full description

Bibliographic Details
Main Authors:	Tengku Abdul Hamid, Tengku Haziyamin, Azhari, Siti Hajar
Format:	Article
Language:	English
Published:	Faculty of Food Science and Technology, Universiti Putra Malaysia 2013
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/26085/ http://irep.iium.edu.my/26085/ http://irep.iium.edu.my/26085/4/Isolation_of_lactic_acid_bacteria_strain_Staphylococcus_piscifermentans_from_Malaysian_traditional_fermented_shrimp_cincaluk.pdf

id	iium-26085
recordtype	eprints
spelling	iium-260852013-02-13T22:44:09Z http://irep.iium.edu.my/26085/ Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk Tengku Abdul Hamid, Tengku Haziyamin Azhari, Siti Hajar Q Science (General) Lactic acid bacteria is well known for it uses as starter culture in various fermented food, and it functions as a good natural antimicrobial agent. Cincaluk, a Malaysian fermented shrimp product commonly found in traditional dishes is commonly enriched with LAB. Out of 50 colonies from a local cincaluk, 7 strains were successfully isolated and shown to be positive in lactose utilization and catalase tests. The majority of the isolates from cincaluk showed Gram-positive cocci morphology and belonged to the group Staphyloccoccus spp. By using agar disc diffusion method, the anti-bacterial properties of these isolates (namely isolate 1, 2, 3, 4, 5, 6, and 7) moderately inhibited the growth of several pathogenic strains, i.e., Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Bacillus subtilis which were used as indicator bacteria. Other than isolates 1, 2, 3 and 5; the 16S rRNA gene for isolate 6 and 7 were successfully amplified. The 16S rRNA gene fragment from isolate 7 was successfully cloned and sequenced. Based on rRNA sequences, both isolates 6 and 7 belonged to the group Staphylococcus piscifermentans, a rare strain previously reported to be specifically isolated exclusive from fish sources. Faculty of Food Science and Technology, Universiti Putra Malaysia 2013-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/26085/4/Isolation_of_lactic_acid_bacteria_strain_Staphylococcus_piscifermentans_from_Malaysian_traditional_fermented_shrimp_cincaluk.pdf Tengku Abdul Hamid, Tengku Haziyamin and Azhari, Siti Hajar (2013) Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk. International Food Research Journal, 20 (1). pp. 125-129. ISSN 19854668 (P), 22317546 (O) http://www.ifrj.upm.edu.my/20%20(01)%202013/18%20IFRJ%2020%20(01)%202013%20Hamid%20(371).pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Tengku Abdul Hamid, Tengku Haziyamin Azhari, Siti Hajar Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk
description	Lactic acid bacteria is well known for it uses as starter culture in various fermented food, and it functions as a good natural antimicrobial agent. Cincaluk, a Malaysian fermented shrimp product commonly found in traditional dishes is commonly enriched with LAB. Out of 50 colonies from a local cincaluk, 7 strains were successfully isolated and shown to be positive in lactose utilization and catalase tests. The majority of the isolates from cincaluk showed Gram-positive cocci morphology and belonged to the group Staphyloccoccus spp. By using agar disc diffusion method, the anti-bacterial properties of these isolates (namely isolate 1, 2, 3, 4, 5, 6, and 7) moderately inhibited the growth of several pathogenic strains, i.e., Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Bacillus subtilis which were used as indicator bacteria. Other than isolates 1, 2, 3 and 5; the 16S rRNA gene for isolate 6 and 7 were successfully amplified. The 16S rRNA gene fragment from isolate 7 was successfully cloned and sequenced. Based on rRNA sequences, both isolates 6 and 7 belonged to the group Staphylococcus piscifermentans, a rare strain previously reported to be specifically isolated exclusive from fish sources.
format	Article
author	Tengku Abdul Hamid, Tengku Haziyamin Azhari, Siti Hajar
author_facet	Tengku Abdul Hamid, Tengku Haziyamin Azhari, Siti Hajar
author_sort	Tengku Abdul Hamid, Tengku Haziyamin
title	Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk
title_short	Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk
title_full	Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk
title_fullStr	Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk
title_full_unstemmed	Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk
title_sort	isolation of lactic acid bacteria strain staphylococcus piscifermentans from malaysian traditional fermented shrimp cincaluk
publisher	Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate	2013
url	http://irep.iium.edu.my/26085/ http://irep.iium.edu.my/26085/ http://irep.iium.edu.my/26085/4/Isolation_of_lactic_acid_bacteria_strain_Staphylococcus_piscifermentans_from_Malaysian_traditional_fermented_shrimp_cincaluk.pdf
first_indexed	2023-09-18T20:38:53Z
last_indexed	2023-09-18T20:38:53Z
_version_	1777409231369011200

Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk

Similar Items