Study on methanolic extracts of Nuclea subdita (Korth) steud. Heartwood parts for the total phenolic contents and free radical scavenging activities
The present study investigated the Total Phenolic Content (TPC) and free radical scavenging activity of Nauclea subdita (Korth) Steud. which might reflect the potency of the plant extract as antioxidant. Young and matured trees of N. subdita were harvested and cut into three heartwood parts (top,...
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Maxwell Scientific Organization
2012
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| Online Access: | http://irep.iium.edu.my/26058/ http://irep.iium.edu.my/26058/ http://irep.iium.edu.my/26058/1/Paper_Antioxidant_Fatin.pdf |
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iium-260582017-06-20T07:52:40Z http://irep.iium.edu.my/26058/ Study on methanolic extracts of Nuclea subdita (Korth) steud. Heartwood parts for the total phenolic contents and free radical scavenging activities Fatin, R. J. Wahab, Razak Mohd. Daud, Jamaludin Sudin, Mahmud Mat Rasat, Mohd Sukhairi Sulaiman, Othman Q Science (General) The present study investigated the Total Phenolic Content (TPC) and free radical scavenging activity of Nauclea subdita (Korth) Steud. which might reflect the potency of the plant extract as antioxidant. Young and matured trees of N. subdita were harvested and cut into three heartwood parts (top, middle and bottom) and grounded into powdered forms. Soxhlet extraction of the heartwood samples using polar (methanol) and non-polar (hexane) solvents and phytochemical screening tests were carried out. The total phenolic contents of the methanolic crude extracts from heartwood parts were determined by colorimetry at 760 nm using Folin-Ciocalteau’s reagent and gallic acid was used as standard. The methanolic extracts were also checked for primary antioxidant activities using an in vitro method, measured by free radical scavenging activity of 2, 2’-Diphenyl-1- Picrylhydrazyl (DPPH) radical. The absorbances were measured at 517 nm by UV/VIS spectrophotometer and compared with standards Trolox and Butylated Hydroxyanisole (BHA). The results from this study suggest that N. subdita heartwood extract may serve as a potential source of natural antioxidant for future development in food and nutraceutical applications. Maxwell Scientific Organization 2012-09-20 Article PeerReviewed application/pdf en http://irep.iium.edu.my/26058/1/Paper_Antioxidant_Fatin.pdf Fatin, R. J. and Wahab, Razak and Mohd. Daud, Jamaludin and Sudin, Mahmud and Mat Rasat, Mohd Sukhairi and Sulaiman, Othman (2012) Study on methanolic extracts of Nuclea subdita (Korth) steud. Heartwood parts for the total phenolic contents and free radical scavenging activities. Current Research Journal of Biological Science, 4 (5). pp. 600-607. ISSN 2041-076X E-ISSN 2041-0778 http://www.maxwellsci.com/print/crjbs/v4-600-607.pdf |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
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IIUM Repository |
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Online Access |
| language |
English |
| topic |
Q Science (General) |
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Q Science (General) Fatin, R. J. Wahab, Razak Mohd. Daud, Jamaludin Sudin, Mahmud Mat Rasat, Mohd Sukhairi Sulaiman, Othman Study on methanolic extracts of Nuclea subdita (Korth) steud. Heartwood parts for the total phenolic contents and free radical scavenging activities |
| description |
The present study investigated the Total Phenolic Content (TPC) and free radical scavenging activity of
Nauclea subdita (Korth) Steud. which might reflect the potency of the plant extract as antioxidant. Young and
matured trees of N. subdita were harvested and cut into three heartwood parts (top, middle and bottom) and
grounded into powdered forms. Soxhlet extraction of the heartwood samples using polar (methanol) and non-polar
(hexane) solvents and phytochemical screening tests were carried out. The total phenolic contents of the methanolic
crude extracts from heartwood parts were determined by colorimetry at 760 nm using Folin-Ciocalteau’s reagent and
gallic acid was used as standard. The methanolic extracts were also checked for primary antioxidant activities using
an in vitro method, measured by free radical scavenging activity of 2, 2’-Diphenyl-1- Picrylhydrazyl (DPPH)
radical. The absorbances were measured at 517 nm by UV/VIS spectrophotometer and compared with standards
Trolox and Butylated Hydroxyanisole (BHA). The results from this study suggest that N. subdita heartwood extract
may serve as a potential source of natural antioxidant for future development in food and nutraceutical applications. |
| format |
Article |
| author |
Fatin, R. J. Wahab, Razak Mohd. Daud, Jamaludin Sudin, Mahmud Mat Rasat, Mohd Sukhairi Sulaiman, Othman |
| author_facet |
Fatin, R. J. Wahab, Razak Mohd. Daud, Jamaludin Sudin, Mahmud Mat Rasat, Mohd Sukhairi Sulaiman, Othman |
| author_sort |
Fatin, R. J. |
| title |
Study on methanolic extracts of Nuclea subdita (Korth) steud. Heartwood parts for the total phenolic contents and free radical scavenging activities |
| title_short |
Study on methanolic extracts of Nuclea subdita (Korth) steud. Heartwood parts for the total phenolic contents and free radical scavenging activities |
| title_full |
Study on methanolic extracts of Nuclea subdita (Korth) steud. Heartwood parts for the total phenolic contents and free radical scavenging activities |
| title_fullStr |
Study on methanolic extracts of Nuclea subdita (Korth) steud. Heartwood parts for the total phenolic contents and free radical scavenging activities |
| title_full_unstemmed |
Study on methanolic extracts of Nuclea subdita (Korth) steud. Heartwood parts for the total phenolic contents and free radical scavenging activities |
| title_sort |
study on methanolic extracts of nuclea subdita (korth) steud. heartwood parts for the total phenolic contents and free radical scavenging activities |
| publisher |
Maxwell Scientific Organization |
| publishDate |
2012 |
| url |
http://irep.iium.edu.my/26058/ http://irep.iium.edu.my/26058/ http://irep.iium.edu.my/26058/1/Paper_Antioxidant_Fatin.pdf |
| first_indexed |
2023-09-18T20:38:51Z |
| last_indexed |
2023-09-18T20:38:51Z |
| _version_ |
1777409229480525824 |