Batch process modelling and economic evaluation of glucuronidase enzyme production
β-glucuronidases enzyme is a valued product in the pharmaceutical industry. This work features the modeling and optimization of β-glucuronidases enzyme production from recombinant E. coli using SuperPro Designer v5, a commercial batch process simulator. The study focuses on designing an economical...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
OMICS Group
2012
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/25805/ http://irep.iium.edu.my/25805/ http://irep.iium.edu.my/25805/1/J_Bioprocess_and_Biotechniques.pdf |
| Summary: | β-glucuronidases enzyme is a valued product in the pharmaceutical industry. This work features the modeling
and optimization of β-glucuronidases enzyme production from recombinant E. coli using SuperPro Designer v5, a
commercial batch process simulator. The study focuses on designing an economically viable β- glucuronidases enzyme
production process. In the base case process, an annual production of 956 batches (corresponding to 8,523 kg) of
β- glucuronidases enzyme was made. Seven alternatives production schemes were further developed for increased
production using batch debottlenecking strategy. The best alternative scheme was reported to achieve a product yield
of 100% increment, with an annual production of 1912 batches of β- glucuronidases enzyme. Economic analysis
determined that the proposed alternative scheme has an annual revenue of USD 44M, with a 15.24% gross margin
and a 29.44% of return on investment. The payback period of this scheme was estimated to be less than four years. |
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