Friedelin and lanosterol from Garcinia prainiana stimulated glucose uptake and adipocytes differentiation in 3T3-L1 adipocytes

Friedelin and lanosterol have been isolated from twigs of Garcinia prainiana.Their structures were elucidated by spectroscopic methods. The compounds were examined for their effects on 3T3-L1 adipocytes. In the MTT assay, it was found that the compounds had no cytotoxic effects up to 25 mM. Adipocy...

Full description

Bibliographic Details
Main Authors:	Darnis, Deny Susanti, Mohamed Amiroudine, Mohamed Zaffar Ali, Rezali, Mohd Fazlin, Bakhtiar, M. Taher
Format:	Article
Language:	English
Published:	Taylor & Francis 2012
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/25776/ http://irep.iium.edu.my/25776/ http://irep.iium.edu.my/25776/ http://irep.iium.edu.my/25776/1/Natural_product_Research.pdf

id	iium-25776
recordtype	eprints
spelling	iium-257762014-12-09T00:24:07Z http://irep.iium.edu.my/25776/ Friedelin and lanosterol from Garcinia prainiana stimulated glucose uptake and adipocytes differentiation in 3T3-L1 adipocytes Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Rezali, Mohd Fazlin Bakhtiar, M. Taher Q Science (General) Friedelin and lanosterol have been isolated from twigs of Garcinia prainiana.Their structures were elucidated by spectroscopic methods. The compounds were examined for their effects on 3T3-L1 adipocytes. In the MTT assay, it was found that the compounds had no cytotoxic effects up to 25 mM. Adipocyte differentiation analysis was carried out by Oil Red O staining method. In the presence of adipogenic cocktail (MDI), it was found that friedelin and lanosterol enhanced intracellular fat accumulation by 2.02 and 2.18-fold, respectively, compared with the vehicle-treated cells. Deoxyglucose uptake assay was used to examine the insulin sensitivity of adipocytes in the presence of the compounds. It was found that friedelin was able to stimulate glucose uptake up to 1.8-fold compared with insulin-treated cells. It was suggested that friedelin and lanosterol may be beneficial to mimic insulin action that would be useful in the treatment of diabetes type 2 patients. Taylor & Francis 2012-09-19 Article PeerReviewed application/pdf en http://irep.iium.edu.my/25776/1/Natural_product_Research.pdf Darnis, Deny Susanti and Mohamed Amiroudine, Mohamed Zaffar Ali and Rezali, Mohd Fazlin and Bakhtiar, M. Taher (2012) Friedelin and lanosterol from Garcinia prainiana stimulated glucose uptake and adipocytes differentiation in 3T3-L1 adipocytes. Natural Product Research, iFirst. pp. 1-8. ISSN 1478-6419 http://www.tandfonline.com/doi/abs/10.1080/14786419.2012.725399?journalCode=gnpl20#preview 10.1080/14786419.2012.725399
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Rezali, Mohd Fazlin Bakhtiar, M. Taher Friedelin and lanosterol from Garcinia prainiana stimulated glucose uptake and adipocytes differentiation in 3T3-L1 adipocytes
description	Friedelin and lanosterol have been isolated from twigs of Garcinia prainiana.Their structures were elucidated by spectroscopic methods. The compounds were examined for their effects on 3T3-L1 adipocytes. In the MTT assay, it was found that the compounds had no cytotoxic effects up to 25 mM. Adipocyte differentiation analysis was carried out by Oil Red O staining method. In the presence of adipogenic cocktail (MDI), it was found that friedelin and lanosterol enhanced intracellular fat accumulation by 2.02 and 2.18-fold, respectively, compared with the vehicle-treated cells. Deoxyglucose uptake assay was used to examine the insulin sensitivity of adipocytes in the presence of the compounds. It was found that friedelin was able to stimulate glucose uptake up to 1.8-fold compared with insulin-treated cells. It was suggested that friedelin and lanosterol may be beneficial to mimic insulin action that would be useful in the treatment of diabetes type 2 patients.
format	Article
author	Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Rezali, Mohd Fazlin Bakhtiar, M. Taher
author_facet	Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Rezali, Mohd Fazlin Bakhtiar, M. Taher
author_sort	Darnis, Deny Susanti
title	Friedelin and lanosterol from Garcinia prainiana stimulated glucose uptake and adipocytes differentiation in 3T3-L1 adipocytes
title_short	Friedelin and lanosterol from Garcinia prainiana stimulated glucose uptake and adipocytes differentiation in 3T3-L1 adipocytes
title_full	Friedelin and lanosterol from Garcinia prainiana stimulated glucose uptake and adipocytes differentiation in 3T3-L1 adipocytes
title_fullStr	Friedelin and lanosterol from Garcinia prainiana stimulated glucose uptake and adipocytes differentiation in 3T3-L1 adipocytes
title_full_unstemmed	Friedelin and lanosterol from Garcinia prainiana stimulated glucose uptake and adipocytes differentiation in 3T3-L1 adipocytes
title_sort	friedelin and lanosterol from garcinia prainiana stimulated glucose uptake and adipocytes differentiation in 3t3-l1 adipocytes
publisher	Taylor & Francis
publishDate	2012
url	http://irep.iium.edu.my/25776/ http://irep.iium.edu.my/25776/ http://irep.iium.edu.my/25776/ http://irep.iium.edu.my/25776/1/Natural_product_Research.pdf
first_indexed	2023-09-18T20:38:23Z
last_indexed	2023-09-18T20:38:23Z
_version_	1777409199864545280

Friedelin and lanosterol from Garcinia prainiana stimulated glucose uptake and adipocytes differentiation in 3T3-L1 adipocytes

Similar Items