Cycloartane triterpenoid as an insulin mimicking agent

The incidence of type 2 diabetes and the resultant metabolic syndrome are rapidly increasing. In this study, we investigated the effects of cycloartane triterpenoids on the adipocyte differentiation and glucose uptake in 3T3-L1 adipocytes together with its mechanism. The cells were treated for lipid...

Full description

Bibliographic Details
Main Authors:	Darnis, Deny Susanti, Mohamed Amiroudine, Mohamed Zaffar Ali, Bakhtiar, M. Taher, Ichwan, Solachuddin J. A., Kaderi, Mohd Arifin
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/25744/ http://irep.iium.edu.my/25744/ http://irep.iium.edu.my/25744/1/Deny_Susanti.pdf

id	iium-25744
recordtype	eprints
spelling	iium-257442014-12-09T01:04:41Z http://irep.iium.edu.my/25744/ Cycloartane triterpenoid as an insulin mimicking agent Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin RS Pharmacy and materia medica The incidence of type 2 diabetes and the resultant metabolic syndrome are rapidly increasing. In this study, we investigated the effects of cycloartane triterpenoids on the adipocyte differentiation and glucose uptake in 3T3-L1 adipocytes together with its mechanism. The cells were treated for lipid accumulation with different concentration of cycloartane by using Oil Red O staining. After day-8 of treatment, morphological changes and high lipid accumulation effects were observed in these cells (p<0.05). In addition, the intracellular fat accumulation increased by 1.9 fold relative to MDI-treated control cells at concentration of 50 µM. The insulin-induced 2-deoxy-D-[3H] glucose uptake also was significantly increased (p<0.01) in cycloartane treated cells as compared to the basal. Moreover, the adipocytes differentiated by cycloartane exhibited insulin sensitivity as demonstrated by a significant increase in glucose uptake. Further evaluation of the mechanism of related gene expression by using the quantitative RT-PCR shows that mature adipocytes treated with cycloartane increased the expression levels of PPARγ which regulate the adipogenesis process and its target gene GLUT4 that involve in glucose uptake. In conclusion, these findings suggest that cycloartane triterpenoids from adipocyte differentiation and glucose uptake by stimulation of PPARγ and GLUT4 expression. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/25744/1/Deny_Susanti.pdf Darnis, Deny Susanti and Mohamed Amiroudine, Mohamed Zaffar Ali and Bakhtiar, M. Taher and Ichwan, Solachuddin J. A. and Kaderi, Mohd Arifin (2012) Cycloartane triterpenoid as an insulin mimicking agent. In: The 24th FAPA Congress 2012, 13-16 September 2012, Bali. http://www.fapa2012.com
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin Cycloartane triterpenoid as an insulin mimicking agent
description	The incidence of type 2 diabetes and the resultant metabolic syndrome are rapidly increasing. In this study, we investigated the effects of cycloartane triterpenoids on the adipocyte differentiation and glucose uptake in 3T3-L1 adipocytes together with its mechanism. The cells were treated for lipid accumulation with different concentration of cycloartane by using Oil Red O staining. After day-8 of treatment, morphological changes and high lipid accumulation effects were observed in these cells (p<0.05). In addition, the intracellular fat accumulation increased by 1.9 fold relative to MDI-treated control cells at concentration of 50 µM. The insulin-induced 2-deoxy-D-[3H] glucose uptake also was significantly increased (p<0.01) in cycloartane treated cells as compared to the basal. Moreover, the adipocytes differentiated by cycloartane exhibited insulin sensitivity as demonstrated by a significant increase in glucose uptake. Further evaluation of the mechanism of related gene expression by using the quantitative RT-PCR shows that mature adipocytes treated with cycloartane increased the expression levels of PPARγ which regulate the adipogenesis process and its target gene GLUT4 that involve in glucose uptake. In conclusion, these findings suggest that cycloartane triterpenoids from adipocyte differentiation and glucose uptake by stimulation of PPARγ and GLUT4 expression.
format	Conference or Workshop Item
author	Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin
author_facet	Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin
author_sort	Darnis, Deny Susanti
title	Cycloartane triterpenoid as an insulin mimicking agent
title_short	Cycloartane triterpenoid as an insulin mimicking agent
title_full	Cycloartane triterpenoid as an insulin mimicking agent
title_fullStr	Cycloartane triterpenoid as an insulin mimicking agent
title_full_unstemmed	Cycloartane triterpenoid as an insulin mimicking agent
title_sort	cycloartane triterpenoid as an insulin mimicking agent
publishDate	2012
url	http://irep.iium.edu.my/25744/ http://irep.iium.edu.my/25744/ http://irep.iium.edu.my/25744/1/Deny_Susanti.pdf
first_indexed	2023-09-18T20:38:20Z
last_indexed	2023-09-18T20:38:20Z
_version_	1777409196196626432

Cycloartane triterpenoid as an insulin mimicking agent

Similar Items