Mangostins stimulate glucose uptake and inhibit adipocyte differentiation in 3T3-L1 adipocytes

Garcinia mangostana (Guttiferae) has interesting biological activities with potential medicinal application. α-mangostin and β-mangostin are the most abundant xanthones isolated from the species. The paper reported the inhibitory effect of the compounds on triglyceride formation, glucose uptake stim...

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Main Authors:	Bakhtiar, M. Taher, Mohamed Amiroudine, Mohamed Zaffar Ali, Darnis, Deny Susanti, Ichwan, Solachuddin J. A., Kaderi, Mohd Arifin
Format:	Conference or Workshop Item
Language:	English English
Published:	2012
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/25743/ http://irep.iium.edu.my/25743/ http://irep.iium.edu.my/25743/3/104Acceptance_ORT1-034_Muhammad_Taher_TB356T.pdf http://irep.iium.edu.my/25743/7/Mangostins_stimulate.pdf

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recordtype	eprints
spelling	iium-257432014-12-09T01:08:11Z http://irep.iium.edu.my/25743/ Mangostins stimulate glucose uptake and inhibit adipocyte differentiation in 3T3-L1 adipocytes Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Darnis, Deny Susanti Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin RS Pharmacy and materia medica Garcinia mangostana (Guttiferae) has interesting biological activities with potential medicinal application. α-mangostin and β-mangostin are the most abundant xanthones isolated from the species. The paper reported the inhibitory effect of the compounds on triglyceride formation, glucose uptake stimulation and gene expression effects on 3T3-L1 adipocytes. Evaluation of the effect of the compounds on triglyceride accumulation was examined by Oil red O staining. The result showed that all compounds inhibited lipid accumulation on 3T3-L1 adipocytes at concentration of 50 μM (P < 0.05) compared to MDI treated cells in a dose-dependent manner. Effect of the cells on uptake of 2-deoxy-D-[3H]glucose was significantly improved by increasing the concentration of the compounds. Analysis of gene expressions by quantitative real-time PCR demonstrated that the compounds inhibited the expression of early adipogenic transcription factor (PPARγ). In addition, the compounds enhanced the expression and plasma membrane translocation of GLUT4 in mature adipocytes. Analysis by using the adipolysis kit showed that α-mangostin particularly increases the free fatty acid release by stimulating the lipolysis pathway. Therefore, these results suggested that α-mangostin and β-mangostin have been found to have a beneficial action in diabetic complications (antiobesity effect) via stimulation of GLUT4 expression and inhibition of PPARγ expression. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/25743/3/104Acceptance_ORT1-034_Muhammad_Taher_TB356T.pdf application/pdf en http://irep.iium.edu.my/25743/7/Mangostins_stimulate.pdf Bakhtiar, M. Taher and Mohamed Amiroudine, Mohamed Zaffar Ali and Darnis, Deny Susanti and Ichwan, Solachuddin J. A. and Kaderi, Mohd Arifin (2012) Mangostins stimulate glucose uptake and inhibit adipocyte differentiation in 3T3-L1 adipocytes. In: The 24th FAPA Congress 2012, 13-16 September 2012, Bali. http://www.fapa2012.com
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Darnis, Deny Susanti Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin Mangostins stimulate glucose uptake and inhibit adipocyte differentiation in 3T3-L1 adipocytes
description	Garcinia mangostana (Guttiferae) has interesting biological activities with potential medicinal application. α-mangostin and β-mangostin are the most abundant xanthones isolated from the species. The paper reported the inhibitory effect of the compounds on triglyceride formation, glucose uptake stimulation and gene expression effects on 3T3-L1 adipocytes. Evaluation of the effect of the compounds on triglyceride accumulation was examined by Oil red O staining. The result showed that all compounds inhibited lipid accumulation on 3T3-L1 adipocytes at concentration of 50 μM (P < 0.05) compared to MDI treated cells in a dose-dependent manner. Effect of the cells on uptake of 2-deoxy-D-[3H]glucose was significantly improved by increasing the concentration of the compounds. Analysis of gene expressions by quantitative real-time PCR demonstrated that the compounds inhibited the expression of early adipogenic transcription factor (PPARγ). In addition, the compounds enhanced the expression and plasma membrane translocation of GLUT4 in mature adipocytes. Analysis by using the adipolysis kit showed that α-mangostin particularly increases the free fatty acid release by stimulating the lipolysis pathway. Therefore, these results suggested that α-mangostin and β-mangostin have been found to have a beneficial action in diabetic complications (antiobesity effect) via stimulation of GLUT4 expression and inhibition of PPARγ expression.
format	Conference or Workshop Item
author	Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Darnis, Deny Susanti Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin
author_facet	Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Darnis, Deny Susanti Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin
author_sort	Bakhtiar, M. Taher
title	Mangostins stimulate glucose uptake and inhibit adipocyte differentiation in 3T3-L1 adipocytes
title_short	Mangostins stimulate glucose uptake and inhibit adipocyte differentiation in 3T3-L1 adipocytes
title_full	Mangostins stimulate glucose uptake and inhibit adipocyte differentiation in 3T3-L1 adipocytes
title_fullStr	Mangostins stimulate glucose uptake and inhibit adipocyte differentiation in 3T3-L1 adipocytes
title_full_unstemmed	Mangostins stimulate glucose uptake and inhibit adipocyte differentiation in 3T3-L1 adipocytes
title_sort	mangostins stimulate glucose uptake and inhibit adipocyte differentiation in 3t3-l1 adipocytes
publishDate	2012
url	http://irep.iium.edu.my/25743/ http://irep.iium.edu.my/25743/ http://irep.iium.edu.my/25743/3/104Acceptance_ORT1-034_Muhammad_Taher_TB356T.pdf http://irep.iium.edu.my/25743/7/Mangostins_stimulate.pdf
first_indexed	2023-09-18T20:38:20Z
last_indexed	2023-09-18T20:38:20Z
_version_	1777409196050874368

Mangostins stimulate glucose uptake and inhibit adipocyte differentiation in 3T3-L1 adipocytes

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