The quadrupole microelectrode design on a multilayer biochip for dielectrophoretic trapping of single cells

This paper introduces a new quadrupolemicroelectrodedesign for trapping mass loading of singlecells using dielectrophoretic (DEP) force. The DEP force profiles generated by the pattern which represent trapping regions on the biochip platform, were studied using finite element software Comsol Multiph...

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Main Author:	Ibrahim, Siti Noorjannah
Format:	Article
Language:	English
Published:	Elsevier 2011
Subjects:	QA75 Electronic computers. Computer science R Medicine (General) TK7885 Computer engineering
Online Access:	http://irep.iium.edu.my/25742/ http://irep.iium.edu.my/25742/ http://irep.iium.edu.my/25742/ http://irep.iium.edu.my/25742/1/The_quadrupole_microelectrode_design_on_a_multilayer_biochip_for_dielectrophoretic_trapping_of_single_cells.pdf

id	iium-25742
recordtype	eprints
spelling	iium-257422013-07-31T04:52:59Z http://irep.iium.edu.my/25742/ The quadrupole microelectrode design on a multilayer biochip for dielectrophoretic trapping of single cells Ibrahim, Siti Noorjannah QA75 Electronic computers. Computer science R Medicine (General) TK7885 Computer engineering This paper introduces a new quadrupolemicroelectrodedesign for trapping mass loading of singlecells using dielectrophoretic (DEP) force. The DEP force profiles generated by the pattern which represent trapping regions on the biochip platform, were studied using finite element software Comsol Multiphysics v3.5a. Arrays of the quadrupolemicroelectrode were patterned on amultilayer structure called sandwiched insulator with back contact (SIBC) biochip platform, fabricated using photolithography technique. This platform consists of a 10 nm Nickel–Chromium (NiCr) and 100 nm Gold (Au) top electrode, 5 μm thick SU-8 2005 insulation layer and a bottom layer of 10 nm NiCr and 100 nm Au called the back contact. Access to the back contact is achieved via a microcavity located at the center of the quadrupolemicroelectrode arrangement. Together, the DEP forces generated from the quadrupolemicroelectrode and the microcavities are used to anchor singlecells and maintain cell positions. The quadrupoledesign was tested with polystyrene latex microbeads, and Ishikawa cancer cells suspended in media and flowed through microfluidic channels made from PDMS. Singlecelltrapping becomes evident when both quadrupolemicroelectrode and back contact are biased using AC potentials of different phases. Experiment results show that the quadrupolemicroelectrode trapped 22 percent of Ishikawa cancer cells and 17 percent of the polystyrene microbeads. Elsevier 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/25742/1/The_quadrupole_microelectrode_design_on_a_multilayer_biochip_for_dielectrophoretic_trapping_of_single_cells.pdf Ibrahim, Siti Noorjannah (2011) The quadrupole microelectrode design on a multilayer biochip for dielectrophoretic trapping of single cells. MIcroelectronic Engineering, 97. pp. 369-374. ISSN 0167-9317 http://www.sciencedirect.com/science/article/pii/S0167931712002006 10.1016/j.mee.2012.04.018
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QA75 Electronic computers. Computer science R Medicine (General) TK7885 Computer engineering
spellingShingle	QA75 Electronic computers. Computer science R Medicine (General) TK7885 Computer engineering Ibrahim, Siti Noorjannah The quadrupole microelectrode design on a multilayer biochip for dielectrophoretic trapping of single cells
description	This paper introduces a new quadrupolemicroelectrodedesign for trapping mass loading of singlecells using dielectrophoretic (DEP) force. The DEP force profiles generated by the pattern which represent trapping regions on the biochip platform, were studied using finite element software Comsol Multiphysics v3.5a. Arrays of the quadrupolemicroelectrode were patterned on amultilayer structure called sandwiched insulator with back contact (SIBC) biochip platform, fabricated using photolithography technique. This platform consists of a 10 nm Nickel–Chromium (NiCr) and 100 nm Gold (Au) top electrode, 5 μm thick SU-8 2005 insulation layer and a bottom layer of 10 nm NiCr and 100 nm Au called the back contact. Access to the back contact is achieved via a microcavity located at the center of the quadrupolemicroelectrode arrangement. Together, the DEP forces generated from the quadrupolemicroelectrode and the microcavities are used to anchor singlecells and maintain cell positions. The quadrupoledesign was tested with polystyrene latex microbeads, and Ishikawa cancer cells suspended in media and flowed through microfluidic channels made from PDMS. Singlecelltrapping becomes evident when both quadrupolemicroelectrode and back contact are biased using AC potentials of different phases. Experiment results show that the quadrupolemicroelectrode trapped 22 percent of Ishikawa cancer cells and 17 percent of the polystyrene microbeads.
format	Article
author	Ibrahim, Siti Noorjannah
author_facet	Ibrahim, Siti Noorjannah
author_sort	Ibrahim, Siti Noorjannah
title	The quadrupole microelectrode design on a multilayer biochip for dielectrophoretic trapping of single cells
title_short	The quadrupole microelectrode design on a multilayer biochip for dielectrophoretic trapping of single cells
title_full	The quadrupole microelectrode design on a multilayer biochip for dielectrophoretic trapping of single cells
title_fullStr	The quadrupole microelectrode design on a multilayer biochip for dielectrophoretic trapping of single cells
title_full_unstemmed	The quadrupole microelectrode design on a multilayer biochip for dielectrophoretic trapping of single cells
title_sort	quadrupole microelectrode design on a multilayer biochip for dielectrophoretic trapping of single cells
publisher	Elsevier
publishDate	2011
url	http://irep.iium.edu.my/25742/ http://irep.iium.edu.my/25742/ http://irep.iium.edu.my/25742/ http://irep.iium.edu.my/25742/1/The_quadrupole_microelectrode_design_on_a_multilayer_biochip_for_dielectrophoretic_trapping_of_single_cells.pdf
first_indexed	2023-09-18T20:38:19Z
last_indexed	2023-09-18T20:38:19Z
_version_	1777409195899879424

The quadrupole microelectrode design on a multilayer biochip for dielectrophoretic trapping of single cells

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