Trapping single cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip

AC electrokinetics is one of many methods used to move particles in microfluidic channels. This paper presents single cell trapping efficacy using dielectrophoresis (DEP) force of two biochip designs; a planar biochip and the new sandwiched-insulation with back contact (SIBC) biochip. The new biochi...

Full description

Bibliographic Details
Main Authors:	Ibrahim, Siti Noorjannah, Murray, Lynn, Evans, John J., M.Alkaisi, Maan
Format:	Article
Language:	English
Published:	Trans Tech Publications 2012
Subjects:	T Technology (General)
Online Access:	http://irep.iium.edu.my/25738/ http://irep.iium.edu.my/25738/ http://irep.iium.edu.my/25738/ http://irep.iium.edu.my/25738/1/Trapping_Single_Cells.pdf

id	iium-25738
recordtype	eprints
spelling	iium-257382013-08-01T04:21:18Z http://irep.iium.edu.my/25738/ Trapping single cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip Ibrahim, Siti Noorjannah Murray, Lynn Evans, John J. M.Alkaisi, Maan T Technology (General) AC electrokinetics is one of many methods used to move particles in microfluidic channels. This paper presents single cell trapping efficacy using dielectrophoresis (DEP) force of two biochip designs; a planar biochip and the new sandwiched-insulation with back contact (SIBC) biochip. The new biochip, is structured on a glass slide, consists of microelectrode arrays patterned on top of Nickel-Chromium (NiCr) and Gold (Au) layers. Prior to the microelectrode patterning, a back contact layer of NiCr and Au was coated with SU-8 2005. Then, the SU-8 2005 or the insulation layer was patterned with arrays of microcavities. In contrast, the planar biochip consists of 2 layers; an SU-8 2005 insulation layer and NiCr and Au metal layers constructed on a Silicon Nitride (Si3N4) substrate. The electric field intensity results simulated using Comsol v3.5a software indicated that DEP force generated from the SIBC biochip are greater than the planar biochip design. Results from experiment with polystyrene microbeads and Ishikawa cancer cells also showed that the SIBC biochip has higher trapping efficiency than the planar biochip. These promising results indicate that the SIBC biochip is capable of trapping single cells and can be used to facilitate studies on intracellular activities using surface the replicating technique known as the Bioimprint technique. Trans Tech Publications 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/25738/1/Trapping_Single_Cells.pdf Ibrahim, Siti Noorjannah and Murray, Lynn and Evans, John J. and M.Alkaisi, Maan (2012) Trapping single cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip. Materials Science Forum, 700. pp. 188-194. ISSN 0255-5476 http://www.scientific.net/MSF.700.188 10.4028/www.scientific.net/MSF.700.188
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General)
spellingShingle	T Technology (General) Ibrahim, Siti Noorjannah Murray, Lynn Evans, John J. M.Alkaisi, Maan Trapping single cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip
description	AC electrokinetics is one of many methods used to move particles in microfluidic channels. This paper presents single cell trapping efficacy using dielectrophoresis (DEP) force of two biochip designs; a planar biochip and the new sandwiched-insulation with back contact (SIBC) biochip. The new biochip, is structured on a glass slide, consists of microelectrode arrays patterned on top of Nickel-Chromium (NiCr) and Gold (Au) layers. Prior to the microelectrode patterning, a back contact layer of NiCr and Au was coated with SU-8 2005. Then, the SU-8 2005 or the insulation layer was patterned with arrays of microcavities. In contrast, the planar biochip consists of 2 layers; an SU-8 2005 insulation layer and NiCr and Au metal layers constructed on a Silicon Nitride (Si3N4) substrate. The electric field intensity results simulated using Comsol v3.5a software indicated that DEP force generated from the SIBC biochip are greater than the planar biochip design. Results from experiment with polystyrene microbeads and Ishikawa cancer cells also showed that the SIBC biochip has higher trapping efficiency than the planar biochip. These promising results indicate that the SIBC biochip is capable of trapping single cells and can be used to facilitate studies on intracellular activities using surface the replicating technique known as the Bioimprint technique.
format	Article
author	Ibrahim, Siti Noorjannah Murray, Lynn Evans, John J. M.Alkaisi, Maan
author_facet	Ibrahim, Siti Noorjannah Murray, Lynn Evans, John J. M.Alkaisi, Maan
author_sort	Ibrahim, Siti Noorjannah
title	Trapping single cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip
title_short	Trapping single cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip
title_full	Trapping single cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip
title_fullStr	Trapping single cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip
title_full_unstemmed	Trapping single cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip
title_sort	trapping single cells: comparison between sandwiched insulation with back contact (sibc) and planar biochip
publisher	Trans Tech Publications
publishDate	2012
url	http://irep.iium.edu.my/25738/ http://irep.iium.edu.my/25738/ http://irep.iium.edu.my/25738/ http://irep.iium.edu.my/25738/1/Trapping_Single_Cells.pdf
first_indexed	2023-09-18T20:38:19Z
last_indexed	2023-09-18T20:38:19Z
_version_	1777409195760418816

Trapping single cells: Comparison between Sandwiched Insulation with Back Contact (SIBC) and Planar Biochip

Similar Items