Anticancer effects of medical Malaysian leech saliva extract (LSE)

Leech saliva contains biologically active compounds that are mainly proteins & peptides. Small cell lung cancer (SCLC) is a form of cell lung carcinoma. In this study a modified and smooth extraction method of saliva was used without leech scarification. Trying to find out the biological activ...

Full description

Bibliographic Details
Main Authors:	Merzouk, Ahmed, Mohammad Ghawi, Abbas, M.Abdualkader, Abdualrahman, Abdullahi, Abubakar Danjuma, Alaama, Mohammed
Format:	Article
Language:	English
Published:	OMICS Publishing Group 2012
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/25003/ http://irep.iium.edu.my/25003/ http://irep.iium.edu.my/25003/ http://irep.iium.edu.my/25003/1/anticancer_effects_of_medical.pdf

id	iium-25003
recordtype	eprints
spelling	iium-250032012-08-02T03:39:09Z http://irep.iium.edu.my/25003/ Anticancer effects of medical Malaysian leech saliva extract (LSE) Merzouk, Ahmed Mohammad Ghawi, Abbas M.Abdualkader, Abdualrahman Abdullahi, Abubakar Danjuma Alaama, Mohammed RS Pharmacy and materia medica Leech saliva contains biologically active compounds that are mainly proteins & peptides. Small cell lung cancer (SCLC) is a form of cell lung carcinoma. In this study a modified and smooth extraction method of saliva was used without leech scarification. Trying to find out the biological activity of medical Malaysian Leech Saliva Extract as cytotoxic in vitro, the SW 1271 cell line was grown and maintained in Leibovitz’s L-15 medium supplemented with 10% foetal bovine serum at 37°C in normal atmospheric air. Serial dilutions of LSE were added to the cell line SW 1271 media for testing the cytotoxic activities.Result revealed that the LSE has a cytotoxic activity against small cell lung cancer(SW 1271 cell line) with IC50 of 119.844 μg/ml compared with IC50 values of two reference standard drugs Irinotecan (5.81 μg/ml) and Carboplatin(18.754 μg/ml). In a combination regimen, LSE reduced the IC50 of Carboplatin & Irinotecan by 65% & 11.5% respectively. Carboplatin reduced the IC50 of LSE by 4.6%, while Irinotecan reduced it by 57%. This results provides a promising novel agent for treatment of small cell lung cancer (SCLC) at least in vitro, more researches are needed OMICS Publishing Group 2012-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/25003/1/anticancer_effects_of_medical.pdf Merzouk, Ahmed and Mohammad Ghawi, Abbas and M.Abdualkader, Abdualrahman and Abdullahi, Abubakar Danjuma and Alaama, Mohammed (2012) Anticancer effects of medical Malaysian leech saliva extract (LSE). Pharmaceutica Analytica Acta, 815. ISSN 2153-2435 http://www.omicsonline.org doi:10.4172/2153-2435.S15-001
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Merzouk, Ahmed Mohammad Ghawi, Abbas M.Abdualkader, Abdualrahman Abdullahi, Abubakar Danjuma Alaama, Mohammed Anticancer effects of medical Malaysian leech saliva extract (LSE)
description	Leech saliva contains biologically active compounds that are mainly proteins & peptides. Small cell lung cancer (SCLC) is a form of cell lung carcinoma. In this study a modified and smooth extraction method of saliva was used without leech scarification. Trying to find out the biological activity of medical Malaysian Leech Saliva Extract as cytotoxic in vitro, the SW 1271 cell line was grown and maintained in Leibovitz’s L-15 medium supplemented with 10% foetal bovine serum at 37°C in normal atmospheric air. Serial dilutions of LSE were added to the cell line SW 1271 media for testing the cytotoxic activities.Result revealed that the LSE has a cytotoxic activity against small cell lung cancer(SW 1271 cell line) with IC50 of 119.844 μg/ml compared with IC50 values of two reference standard drugs Irinotecan (5.81 μg/ml) and Carboplatin(18.754 μg/ml). In a combination regimen, LSE reduced the IC50 of Carboplatin & Irinotecan by 65% & 11.5% respectively. Carboplatin reduced the IC50 of LSE by 4.6%, while Irinotecan reduced it by 57%. This results provides a promising novel agent for treatment of small cell lung cancer (SCLC) at least in vitro, more researches are needed
format	Article
author	Merzouk, Ahmed Mohammad Ghawi, Abbas M.Abdualkader, Abdualrahman Abdullahi, Abubakar Danjuma Alaama, Mohammed
author_facet	Merzouk, Ahmed Mohammad Ghawi, Abbas M.Abdualkader, Abdualrahman Abdullahi, Abubakar Danjuma Alaama, Mohammed
author_sort	Merzouk, Ahmed
title	Anticancer effects of medical Malaysian leech saliva extract (LSE)
title_short	Anticancer effects of medical Malaysian leech saliva extract (LSE)
title_full	Anticancer effects of medical Malaysian leech saliva extract (LSE)
title_fullStr	Anticancer effects of medical Malaysian leech saliva extract (LSE)
title_full_unstemmed	Anticancer effects of medical Malaysian leech saliva extract (LSE)
title_sort	anticancer effects of medical malaysian leech saliva extract (lse)
publisher	OMICS Publishing Group
publishDate	2012
url	http://irep.iium.edu.my/25003/ http://irep.iium.edu.my/25003/ http://irep.iium.edu.my/25003/ http://irep.iium.edu.my/25003/1/anticancer_effects_of_medical.pdf
first_indexed	2023-09-18T20:37:23Z
last_indexed	2023-09-18T20:37:23Z
_version_	1777409136718249984

Anticancer effects of medical Malaysian leech saliva extract (LSE)

Similar Items