TAL - a source of bacterial endotoxin detector in liquid biological samples

Endotoxins (chemically known as Lipopolysaccharide) from gram-negative microorganisms initiates clot formation in blood when it is accidentally encountered by horseshoe crab blood stream. This property was extensively studied by various researchers as a result Limulus Amebocyte Lysate (LAL) test was...

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Main Authors:	John, Akbar, Yunus, Kamaruzzaman, Khan Chowdhury, Ahmed Jalal, Kassim, Zaleha
Format:	Article
Language:	English
Published:	Faculty of Food Science and Technology, Universiti Putra Malaysia 2012
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/24964/ http://irep.iium.edu.my/24964/ http://irep.iium.edu.my/24964/1/paper_2.pdf

id	iium-24964
recordtype	eprints
spelling	iium-249642017-12-07T05:52:03Z http://irep.iium.edu.my/24964/ TAL - a source of bacterial endotoxin detector in liquid biological samples John, Akbar Yunus, Kamaruzzaman Khan Chowdhury, Ahmed Jalal Kassim, Zaleha Q Science (General) Endotoxins (chemically known as Lipopolysaccharide) from gram-negative microorganisms initiates clot formation in blood when it is accidentally encountered by horseshoe crab blood stream. This property was extensively studied by various researchers as a result Limulus Amebocyte Lysate (LAL) test was established. The LAL tests in general, 3 to 300 times more sensitive than the United States Pharmacopeial (USP) rabbit pyrogen test method. It is apparent that major differences among the LAL preparations lie in the area of sensitivity. Differences, up to 100-fold, exist in the sensitivity of the various LAL preparations to the same endotoxin. Based on the above perspective, a portable Kit (Endo sensor) was developed to detect the presence of bacterial endotoxin in liquid biological samples using Tachypleus Amebocyte Lysate (TAL) as a source. Sensitivity of the Kit was determined using various concentrations of prepared endotoxin standards and pyrogen free water samples. It was observed that Endo sensor could detect up to nano gram level of endotoxin in liquid biological samples which could be expressed in (EU/ml) and the labeled sensitivity of the lysated product was 0.125 EU/ ml. The gel clotting principle method was utilized for the detection of bacterial endotoxin in liquid biological samples. Faculty of Food Science and Technology, Universiti Putra Malaysia 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/24964/1/paper_2.pdf John, Akbar and Yunus, Kamaruzzaman and Khan Chowdhury, Ahmed Jalal and Kassim, Zaleha (2012) TAL - a source of bacterial endotoxin detector in liquid biological samples. International Food Research Journal, 19 (2). pp. 423-425. ISSN 19854668 (P), 22317546 (O) http://www.ifrj.upm.edu.my/19%20(02)%202012/(6)IFRJ-2012%20Akbar.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) John, Akbar Yunus, Kamaruzzaman Khan Chowdhury, Ahmed Jalal Kassim, Zaleha TAL - a source of bacterial endotoxin detector in liquid biological samples
description	Endotoxins (chemically known as Lipopolysaccharide) from gram-negative microorganisms initiates clot formation in blood when it is accidentally encountered by horseshoe crab blood stream. This property was extensively studied by various researchers as a result Limulus Amebocyte Lysate (LAL) test was established. The LAL tests in general, 3 to 300 times more sensitive than the United States Pharmacopeial (USP) rabbit pyrogen test method. It is apparent that major differences among the LAL preparations lie in the area of sensitivity. Differences, up to 100-fold, exist in the sensitivity of the various LAL preparations to the same endotoxin. Based on the above perspective, a portable Kit (Endo sensor) was developed to detect the presence of bacterial endotoxin in liquid biological samples using Tachypleus Amebocyte Lysate (TAL) as a source. Sensitivity of the Kit was determined using various concentrations of prepared endotoxin standards and pyrogen free water samples. It was observed that Endo sensor could detect up to nano gram level of endotoxin in liquid biological samples which could be expressed in (EU/ml) and the labeled sensitivity of the lysated product was 0.125 EU/ ml. The gel clotting principle method was utilized for the detection of bacterial endotoxin in liquid biological samples.
format	Article
author	John, Akbar Yunus, Kamaruzzaman Khan Chowdhury, Ahmed Jalal Kassim, Zaleha
author_facet	John, Akbar Yunus, Kamaruzzaman Khan Chowdhury, Ahmed Jalal Kassim, Zaleha
author_sort	John, Akbar
title	TAL - a source of bacterial endotoxin detector in liquid biological samples
title_short	TAL - a source of bacterial endotoxin detector in liquid biological samples
title_full	TAL - a source of bacterial endotoxin detector in liquid biological samples
title_fullStr	TAL - a source of bacterial endotoxin detector in liquid biological samples
title_full_unstemmed	TAL - a source of bacterial endotoxin detector in liquid biological samples
title_sort	tal - a source of bacterial endotoxin detector in liquid biological samples
publisher	Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate	2012
url	http://irep.iium.edu.my/24964/ http://irep.iium.edu.my/24964/ http://irep.iium.edu.my/24964/1/paper_2.pdf
first_indexed	2023-09-18T20:37:19Z
last_indexed	2023-09-18T20:37:19Z
_version_	1777409132680183808

TAL - a source of bacterial endotoxin detector in liquid biological samples

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