Effects of Palm-tocotrienol Rich Fraction (TRF) on OATP2 protein expression in Bilirubin Transport in adult rats

Effects of Palm-tocotrienol Rich Fraction (TRF) on OATP2 protein expression in Bilirubin Transport in adult rats

OATP2 is a transport protein which is involved in bilirubin metabolism and excretion. Palm-tocotrienol rich fraction (TRF) has previously been shown to reduce total plasma bilirubin (TB). This study explored the effects of TRF pre-treatment on phenylhydrazine (PHZ) induced hyperbilirubinaemia in adu...

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Main Authors: Ismawi, Hidayatul Radziah, Mohd Saad, Qodriyah, Ahmad Yusof, Asmadi, Othman, Faizah, Yusuf, Kamisah
Format: Conference or Workshop Item
Language:English
Published: 2011
Subjects:
RM Therapeutics. Pharmacology
Online Access:http://irep.iium.edu.my/24949/
http://irep.iium.edu.my/24949/5/PINCcover.pdf
id iium-24949
recordtype eprints
spelling iium-249492013-09-17T03:28:37Z http://irep.iium.edu.my/24949/ Effects of Palm-tocotrienol Rich Fraction (TRF) on OATP2 protein expression in Bilirubin Transport in adult rats Ismawi, Hidayatul Radziah Mohd Saad, Qodriyah Ahmad Yusof, Asmadi Othman, Faizah Yusuf, Kamisah RM Therapeutics. Pharmacology OATP2 is a transport protein which is involved in bilirubin metabolism and excretion. Palm-tocotrienol rich fraction (TRF) has previously been shown to reduce total plasma bilirubin (TB). This study explored the effects of TRF pre-treatment on phenylhydrazine (PHZ) induced hyperbilirubinaemia in adult rats on bilirubin transport protein expression. Thirty-six adult male Wistar rats (200-250g) were divided into three groups; control (C), phenobarbital (PB) and TRF. TRF (100mg/kg/day, i.p.) and control (vitamin E free palm oil, i.p.) were given from the first day to the fourteenth day of study. For the PB group, normal saline (NS) was given for the first nine days and PB (80mg/kg/day, i.p.) was given for the next five days. On day 15, all the groups were further subdivided into two smaller groups. One group was given NS and the other group was given PHZ (C+NS, C+PHZ; PB+NS, PB+PHZ; TRF+NS, TRF+PHZ). After 24 hours, plasma TB was measured and liver tissue was taken for protein expression using Western Blot technique. TB was significantly higher in the C+PHZ group (0.19mg/dL +- 0.026) compared to C+NS group (0.06 mg/dL +- 0.008) TRF and PB did not reduce TB in PHZ-induced groups compared to the C+PHZ group. However, TB in the TRF+NS group was significantly lower compared to the C+NS and PB+NS groups. Treatment with TRF and phenobarbital increased the expressions of OATP2 in all groups compared to the control. In conclusion, TRF and PB increased the expressions of OATP2. However, TRF did not affect plasma TB in rats induced with PHZ. 2011 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/24949/5/PINCcover.pdf Ismawi, Hidayatul Radziah and Mohd Saad, Qodriyah and Ahmad Yusof, Asmadi and Othman, Faizah and Yusuf, Kamisah (2011) Effects of Palm-tocotrienol Rich Fraction (TRF) on OATP2 protein expression in Bilirubin Transport in adult rats. In: Palm International Nutra-Cosmeceutical Conference, 19-20 May 2011, Crown Plaza Mutiara Hotel, Kuala Lumpur.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic RM Therapeutics. Pharmacology
spellingShingle RM Therapeutics. Pharmacology
Ismawi, Hidayatul Radziah
Mohd Saad, Qodriyah
Ahmad Yusof, Asmadi
Othman, Faizah
Yusuf, Kamisah
Effects of Palm-tocotrienol Rich Fraction (TRF) on OATP2 protein expression in Bilirubin Transport in adult rats
description OATP2 is a transport protein which is involved in bilirubin metabolism and excretion. Palm-tocotrienol rich fraction (TRF) has previously been shown to reduce total plasma bilirubin (TB). This study explored the effects of TRF pre-treatment on phenylhydrazine (PHZ) induced hyperbilirubinaemia in adult rats on bilirubin transport protein expression. Thirty-six adult male Wistar rats (200-250g) were divided into three groups; control (C), phenobarbital (PB) and TRF. TRF (100mg/kg/day, i.p.) and control (vitamin E free palm oil, i.p.) were given from the first day to the fourteenth day of study. For the PB group, normal saline (NS) was given for the first nine days and PB (80mg/kg/day, i.p.) was given for the next five days. On day 15, all the groups were further subdivided into two smaller groups. One group was given NS and the other group was given PHZ (C+NS, C+PHZ; PB+NS, PB+PHZ; TRF+NS, TRF+PHZ). After 24 hours, plasma TB was measured and liver tissue was taken for protein expression using Western Blot technique. TB was significantly higher in the C+PHZ group (0.19mg/dL +- 0.026) compared to C+NS group (0.06 mg/dL +- 0.008) TRF and PB did not reduce TB in PHZ-induced groups compared to the C+PHZ group. However, TB in the TRF+NS group was significantly lower compared to the C+NS and PB+NS groups. Treatment with TRF and phenobarbital increased the expressions of OATP2 in all groups compared to the control. In conclusion, TRF and PB increased the expressions of OATP2. However, TRF did not affect plasma TB in rats induced with PHZ.
format Conference or Workshop Item
author Ismawi, Hidayatul Radziah
Mohd Saad, Qodriyah
Ahmad Yusof, Asmadi
Othman, Faizah
Yusuf, Kamisah
author_facet Ismawi, Hidayatul Radziah
Mohd Saad, Qodriyah
Ahmad Yusof, Asmadi
Othman, Faizah
Yusuf, Kamisah
author_sort Ismawi, Hidayatul Radziah
title Effects of Palm-tocotrienol Rich Fraction (TRF) on OATP2 protein expression in Bilirubin Transport in adult rats
title_short Effects of Palm-tocotrienol Rich Fraction (TRF) on OATP2 protein expression in Bilirubin Transport in adult rats
title_full Effects of Palm-tocotrienol Rich Fraction (TRF) on OATP2 protein expression in Bilirubin Transport in adult rats
title_fullStr Effects of Palm-tocotrienol Rich Fraction (TRF) on OATP2 protein expression in Bilirubin Transport in adult rats
title_full_unstemmed Effects of Palm-tocotrienol Rich Fraction (TRF) on OATP2 protein expression in Bilirubin Transport in adult rats
title_sort effects of palm-tocotrienol rich fraction (trf) on oatp2 protein expression in bilirubin transport in adult rats
publishDate 2011
url http://irep.iium.edu.my/24949/
http://irep.iium.edu.my/24949/5/PINCcover.pdf
first_indexed 2023-09-18T20:37:18Z
last_indexed 2023-09-18T20:37:18Z
_version_ 1777409131656773632

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