Hydrolysis of granular starch at sub-gelatinization temperature using a mixture of amylolytic enzymes

Native granular starches (corn, cassava,mung bean, and sago)were hydrolyzed using a mixture of alpha-amylase and glucoamylase at 35 ◦C for 24 h. Hydrolyzed starches were analyzed for the degree of hydrolysis and for physicochemical and functional properties. Corn starch showed the highest degree of...

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Main Authors: U., Uthumporn, Sarker, Md. Zaidul Islam, Al-Jashamy, Karim A.
Format: Article
Language:English
Published: Elsevier Inc. 2010
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Online Access:http://irep.iium.edu.my/24840/
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spelling iium-248402013-02-13T10:27:08Z http://irep.iium.edu.my/24840/ Hydrolysis of granular starch at sub-gelatinization temperature using a mixture of amylolytic enzymes U., Uthumporn Sarker, Md. Zaidul Islam Al-Jashamy, Karim A. QD Chemistry Native granular starches (corn, cassava,mung bean, and sago)were hydrolyzed using a mixture of alpha-amylase and glucoamylase at 35 ◦C for 24 h. Hydrolyzed starches were analyzed for the degree of hydrolysis and for physicochemical and functional properties. Corn starch showed the highest degree of hydrolysis, as evidenced by the presence of distinct pores penetrating deep into the granules. Enzymatic erosion occurred mainly at the surface for cassava, whereas isolated porous structures were observed in hydrolyzed mung bean and sago starch. The amylose content was significantly lower in all starches except for sago starch. The powder X-ray diffraction of all starches showed no significant changes after hydrolysis, but hydrolyzed starches showed a more crystalline nature. The action of enzymes caused significant changes in some pasting properties and in the swelling/solubility of starches. Evidently, enzymes were able to hydrolyze granular starches to a variable degree at sub-gelatinization temperature, and produced a relatively high degree of conversion. Elsevier Inc. 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/24840/1/S32IJTRX1291773482921.pdf U., Uthumporn and Sarker, Md. Zaidul Islam and Al-Jashamy, Karim A. (2010) Hydrolysis of granular starch at sub-gelatinization temperature using a mixture of amylolytic enzymes. Food and Bioproducts Processing, 88 (1). pp. 47-54. ISSN 09603085 http://dx.doi.org/10.1016/j.fbp.2009.10.001 doi:10.1016/j.fbp.2009.10.001
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
U., Uthumporn
Sarker, Md. Zaidul Islam
Al-Jashamy, Karim A.
Hydrolysis of granular starch at sub-gelatinization temperature using a mixture of amylolytic enzymes
description Native granular starches (corn, cassava,mung bean, and sago)were hydrolyzed using a mixture of alpha-amylase and glucoamylase at 35 ◦C for 24 h. Hydrolyzed starches were analyzed for the degree of hydrolysis and for physicochemical and functional properties. Corn starch showed the highest degree of hydrolysis, as evidenced by the presence of distinct pores penetrating deep into the granules. Enzymatic erosion occurred mainly at the surface for cassava, whereas isolated porous structures were observed in hydrolyzed mung bean and sago starch. The amylose content was significantly lower in all starches except for sago starch. The powder X-ray diffraction of all starches showed no significant changes after hydrolysis, but hydrolyzed starches showed a more crystalline nature. The action of enzymes caused significant changes in some pasting properties and in the swelling/solubility of starches. Evidently, enzymes were able to hydrolyze granular starches to a variable degree at sub-gelatinization temperature, and produced a relatively high degree of conversion.
format Article
author U., Uthumporn
Sarker, Md. Zaidul Islam
Al-Jashamy, Karim A.
author_facet U., Uthumporn
Sarker, Md. Zaidul Islam
Al-Jashamy, Karim A.
author_sort U., Uthumporn
title Hydrolysis of granular starch at sub-gelatinization temperature using a mixture of amylolytic enzymes
title_short Hydrolysis of granular starch at sub-gelatinization temperature using a mixture of amylolytic enzymes
title_full Hydrolysis of granular starch at sub-gelatinization temperature using a mixture of amylolytic enzymes
title_fullStr Hydrolysis of granular starch at sub-gelatinization temperature using a mixture of amylolytic enzymes
title_full_unstemmed Hydrolysis of granular starch at sub-gelatinization temperature using a mixture of amylolytic enzymes
title_sort hydrolysis of granular starch at sub-gelatinization temperature using a mixture of amylolytic enzymes
publisher Elsevier Inc.
publishDate 2010
url http://irep.iium.edu.my/24840/
http://irep.iium.edu.my/24840/
http://irep.iium.edu.my/24840/
http://irep.iium.edu.my/24840/1/S32IJTRX1291773482921.pdf
first_indexed 2023-09-18T20:37:08Z
last_indexed 2023-09-18T20:37:08Z
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