Transmission electron microscopical assessment of black cumin fixed oil treatment on rat's hippocampus with surgically induced neurodegeneration

A growing body of evidence now supports the hypothesis that critically attained threshold cerebral hypoperfusion (CATCH) accelerates age related neurodegeneration. Such threshold was achieved in rats by permanent bilateral common carotid artery occlusion (2VO). The hippocampal CA1 region pyramidal c...

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Main Authors:	Azzubaidi, Marwan Saad, Talib, Norlelawati A., Saxena, Anil Kumar, Alattraqchi, Ahmed G., Maideen, Mohd Maizam
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Subjects:	R Medicine (General) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/24797/ http://irep.iium.edu.my/24797/1/Our_conference_participation.pdf

id	iium-24797
recordtype	eprints
spelling	iium-247972012-12-17T09:53:27Z http://irep.iium.edu.my/24797/ Transmission electron microscopical assessment of black cumin fixed oil treatment on rat's hippocampus with surgically induced neurodegeneration Azzubaidi, Marwan Saad Talib, Norlelawati A. Saxena, Anil Kumar Alattraqchi, Ahmed G. Maideen, Mohd Maizam R Medicine (General) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action A growing body of evidence now supports the hypothesis that critically attained threshold cerebral hypoperfusion (CATCH) accelerates age related neurodegeneration. Such threshold was achieved in rats by permanent bilateral common carotid artery occlusion (2VO). The hippocampal CA1 region pyramidal cells are the earliest neurons to degenerate following 2VO intervention. Nigella sativa oil extract (NSO) was found to preserve viability of cerebellar neuron cells in vitro putting forward its potential neuroprotective effect. The study objective was to assess the effect of NSO treatment on hippocampal pyramidal and other neighboring cells within CA1 subfield of rats with CATCH that was achieved through 2VO procedure. Nine rats were equally divided into three groups: sham control, untreated 2VO and NSO treated group (2VO with daily oral NSO treatment). After the 10th postoperative week coronal sections of the hippocampus were collected and processed for transmission electron microscopy (TEM). The neurodegenerative changes observed in pyramidal cells, astrocytes, endothelial basement membrane and axoplasms within CA1 hippocampal region of 2VO group were not observed in sham control group. Meanwhile, NSO treated groups hardly showed ultrastructural neurodegenerative changes similar to those observed in untreated 2VO group. This indicates a promising prospective neuroprotective activity provided by NSO treatment to prevent age related neurodegenerative diseases especially Alzheimer’s disease. Keywords: Neurodegeneration, Alzheimer, hippocampus, 2VO, Nigella sativa, Transmission electron microscopy, rat. 2012 Conference or Workshop Item PeerReviewed application/pdf en cc_by http://irep.iium.edu.my/24797/1/Our_conference_participation.pdf Azzubaidi, Marwan Saad and Talib, Norlelawati A. and Saxena, Anil Kumar and Alattraqchi, Ahmed G. and Maideen, Mohd Maizam (2012) Transmission electron microscopical assessment of black cumin fixed oil treatment on rat's hippocampus with surgically induced neurodegeneration. In: 26th Scientific meeting of Malaysian Society of Pharmacology and Physiology, 18 - 20 May 2012, Penang.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action
spellingShingle	R Medicine (General) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action Azzubaidi, Marwan Saad Talib, Norlelawati A. Saxena, Anil Kumar Alattraqchi, Ahmed G. Maideen, Mohd Maizam Transmission electron microscopical assessment of black cumin fixed oil treatment on rat's hippocampus with surgically induced neurodegeneration
description	A growing body of evidence now supports the hypothesis that critically attained threshold cerebral hypoperfusion (CATCH) accelerates age related neurodegeneration. Such threshold was achieved in rats by permanent bilateral common carotid artery occlusion (2VO). The hippocampal CA1 region pyramidal cells are the earliest neurons to degenerate following 2VO intervention. Nigella sativa oil extract (NSO) was found to preserve viability of cerebellar neuron cells in vitro putting forward its potential neuroprotective effect. The study objective was to assess the effect of NSO treatment on hippocampal pyramidal and other neighboring cells within CA1 subfield of rats with CATCH that was achieved through 2VO procedure. Nine rats were equally divided into three groups: sham control, untreated 2VO and NSO treated group (2VO with daily oral NSO treatment). After the 10th postoperative week coronal sections of the hippocampus were collected and processed for transmission electron microscopy (TEM). The neurodegenerative changes observed in pyramidal cells, astrocytes, endothelial basement membrane and axoplasms within CA1 hippocampal region of 2VO group were not observed in sham control group. Meanwhile, NSO treated groups hardly showed ultrastructural neurodegenerative changes similar to those observed in untreated 2VO group. This indicates a promising prospective neuroprotective activity provided by NSO treatment to prevent age related neurodegenerative diseases especially Alzheimer’s disease. Keywords: Neurodegeneration, Alzheimer, hippocampus, 2VO, Nigella sativa, Transmission electron microscopy, rat.
format	Conference or Workshop Item
author	Azzubaidi, Marwan Saad Talib, Norlelawati A. Saxena, Anil Kumar Alattraqchi, Ahmed G. Maideen, Mohd Maizam
author_facet	Azzubaidi, Marwan Saad Talib, Norlelawati A. Saxena, Anil Kumar Alattraqchi, Ahmed G. Maideen, Mohd Maizam
author_sort	Azzubaidi, Marwan Saad
title	Transmission electron microscopical assessment of black cumin fixed oil treatment on rat's hippocampus with surgically induced neurodegeneration
title_short	Transmission electron microscopical assessment of black cumin fixed oil treatment on rat's hippocampus with surgically induced neurodegeneration
title_full	Transmission electron microscopical assessment of black cumin fixed oil treatment on rat's hippocampus with surgically induced neurodegeneration
title_fullStr	Transmission electron microscopical assessment of black cumin fixed oil treatment on rat's hippocampus with surgically induced neurodegeneration
title_full_unstemmed	Transmission electron microscopical assessment of black cumin fixed oil treatment on rat's hippocampus with surgically induced neurodegeneration
title_sort	transmission electron microscopical assessment of black cumin fixed oil treatment on rat's hippocampus with surgically induced neurodegeneration
publishDate	2012
url	http://irep.iium.edu.my/24797/ http://irep.iium.edu.my/24797/1/Our_conference_participation.pdf
first_indexed	2023-09-18T20:37:06Z
last_indexed	2023-09-18T20:37:06Z
_version_	1777409118880923648

Transmission electron microscopical assessment of black cumin fixed oil treatment on rat's hippocampus with surgically induced neurodegeneration

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