Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil

Laboratory study was carried out to isolate hydrocarbon utilizing bacteria from crude-oil contaminated soil (COCS) to test their potential for the bioremediation of COCS. Twenty strains were isolated by enrichment culture using minimal salt medium with crude oil as sole carbon source. The twenty iso...

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Main Authors:	Husain, Iman A. F., Al-Khatib, Ma An Fahmi Rashid, Alam, Md. Zahangir, Muyibi, Suleyman Aremu
Format:	Article
Language:	English
Published:	AJBAS Journal 2011
Subjects:	TD172 Environmental pollution TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/2478/ http://irep.iium.edu.my/2478/ http://irep.iium.edu.my/2478/1/AJBAS_2011_925-930.pdf

id	iium-2478
recordtype	eprints
spelling	iium-24782012-02-28T09:28:02Z http://irep.iium.edu.my/2478/ Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil Husain, Iman A. F. Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu TD172 Environmental pollution TP248.13 Biotechnology Laboratory study was carried out to isolate hydrocarbon utilizing bacteria from crude-oil contaminated soil (COCS) to test their potential for the bioremediation of COCS. Twenty strains were isolated by enrichment culture using minimal salt medium with crude oil as sole carbon source. The twenty isolated strains were purified and monitored for their growth by monitoring the optical density (OD600). The twelve best growing strains were selected to form four bacterial consortiums. Each consortium contains three strains combined with accordance to their growth. The four consortiums were tested for their growth and the best one (Cons.1) was selected for the kinetics study and the biodegradability test. From the first order kinetic study, the maximum growth rate μmax of the selected consortium was 0.02 hr-1 while the half saturation constant ks was 2.2%v/v total petroleum hydrocarbons (TPH). The selected strains showed a potential for biodegradation of crude oil in soil slurry with removal up to 95% within seven days which would further be used for the bioremediation of COCS. AJBAS Journal 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/2478/1/AJBAS_2011_925-930.pdf Husain, Iman A. F. and Al-Khatib, Ma An Fahmi Rashid and Alam, Md. Zahangir and Muyibi, Suleyman Aremu (2011) Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil. Australian Journal of Basic and Applied Sciences, 5 (6). pp. 925-930. ISSN 1991-8178 http://www.insipub.com/ajbas.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TD172 Environmental pollution TP248.13 Biotechnology
spellingShingle	TD172 Environmental pollution TP248.13 Biotechnology Husain, Iman A. F. Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil
description	Laboratory study was carried out to isolate hydrocarbon utilizing bacteria from crude-oil contaminated soil (COCS) to test their potential for the bioremediation of COCS. Twenty strains were isolated by enrichment culture using minimal salt medium with crude oil as sole carbon source. The twenty isolated strains were purified and monitored for their growth by monitoring the optical density (OD600). The twelve best growing strains were selected to form four bacterial consortiums. Each consortium contains three strains combined with accordance to their growth. The four consortiums were tested for their growth and the best one (Cons.1) was selected for the kinetics study and the biodegradability test. From the first order kinetic study, the maximum growth rate μmax of the selected consortium was 0.02 hr-1 while the half saturation constant ks was 2.2%v/v total petroleum hydrocarbons (TPH). The selected strains showed a potential for biodegradation of crude oil in soil slurry with removal up to 95% within seven days which would further be used for the bioremediation of COCS.
format	Article
author	Husain, Iman A. F. Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu
author_facet	Husain, Iman A. F. Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu
author_sort	Husain, Iman A. F.
title	Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil
title_short	Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil
title_full	Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil
title_fullStr	Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil
title_full_unstemmed	Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil
title_sort	kinetic study of a bacterial consortium isolated from soil contaminated with crude oil
publisher	AJBAS Journal
publishDate	2011
url	http://irep.iium.edu.my/2478/ http://irep.iium.edu.my/2478/ http://irep.iium.edu.my/2478/1/AJBAS_2011_925-930.pdf
first_indexed	2023-09-18T20:10:04Z
last_indexed	2023-09-18T20:10:04Z
_version_	1777407418459750400

Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil

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