Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa

Study on biotransformation of �-fatty acid from fish oil was carried out. Pseudomonas aeruginosa has been chosen to be the biocatalyst for biotransformation of w fatty acids extracted from Malaysian catfish, Clarias gariepinus. The lipid from freeze-dried catfish flesh was extracted using a modifi...

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Main Authors:	Omar, Muhammad Nor, Shaban, Norhayati, Bakar, Latifah Munirah, Zuberdi, Ahmad Muzammil
Format:	Article
Language:	English
Published:	Academic Information Press 2012
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/24742/ http://irep.iium.edu.my/24742/ http://irep.iium.edu.my/24742/1/Biotransformation_of_Malaysian_Catfish_Innova_Ceincia.pdf

id	iium-24742
recordtype	eprints
spelling	iium-247422012-08-13T01:04:37Z http://irep.iium.edu.my/24742/ Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa Omar, Muhammad Nor Shaban, Norhayati Bakar, Latifah Munirah Zuberdi, Ahmad Muzammil QD Chemistry Study on biotransformation of �-fatty acid from fish oil was carried out. Pseudomonas aeruginosa has been chosen to be the biocatalyst for biotransformation of w fatty acids extracted from Malaysian catfish, Clarias gariepinus. The lipid from freeze-dried catfish flesh was extracted using a modified Folch method and the mixture of chloroform and methanol was used as a solvent. The crude lipid substrate was added to the bacterial culture and incubated for 4 days. After conversion, the products were analyzed by using gas chromatography and mass spectrometer (GC-MS). The analytical result showed that several fatty acids and cholesterol were found in the product. However, the fatty acids and cholesterol contents before and after biotransformation were different. The cholesterol content increased while �-fatty acid contents decreased after biotransformation process. It can be concluded that the bacterial cells had oxidized the �-fatty acids to yield precursors which can be utilized as starting materials for the bioconversion to cholesterol. Academic Information Press 2012-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/24742/1/Biotransformation_of_Malaysian_Catfish_Innova_Ceincia.pdf Omar, Muhammad Nor and Shaban, Norhayati and Bakar, Latifah Munirah and Zuberdi, Ahmad Muzammil (2012) Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa. Innova Ciencia, 4 (6). pp. 82-88. ISSN 1333-114X http://www.ic.sciencerecord.com/archive/index.php/?volume=4&issue=6
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Omar, Muhammad Nor Shaban, Norhayati Bakar, Latifah Munirah Zuberdi, Ahmad Muzammil Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa
description	Study on biotransformation of �-fatty acid from fish oil was carried out. Pseudomonas aeruginosa has been chosen to be the biocatalyst for biotransformation of w fatty acids extracted from Malaysian catfish, Clarias gariepinus. The lipid from freeze-dried catfish flesh was extracted using a modified Folch method and the mixture of chloroform and methanol was used as a solvent. The crude lipid substrate was added to the bacterial culture and incubated for 4 days. After conversion, the products were analyzed by using gas chromatography and mass spectrometer (GC-MS). The analytical result showed that several fatty acids and cholesterol were found in the product. However, the fatty acids and cholesterol contents before and after biotransformation were different. The cholesterol content increased while �-fatty acid contents decreased after biotransformation process. It can be concluded that the bacterial cells had oxidized the �-fatty acids to yield precursors which can be utilized as starting materials for the bioconversion to cholesterol.
format	Article
author	Omar, Muhammad Nor Shaban, Norhayati Bakar, Latifah Munirah Zuberdi, Ahmad Muzammil
author_facet	Omar, Muhammad Nor Shaban, Norhayati Bakar, Latifah Munirah Zuberdi, Ahmad Muzammil
author_sort	Omar, Muhammad Nor
title	Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa
title_short	Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa
title_full	Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa
title_fullStr	Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa
title_full_unstemmed	Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa
title_sort	biotransformation of ω-fatty acid from clarias gariepinus using psuedomonas aeruginosa
publisher	Academic Information Press
publishDate	2012
url	http://irep.iium.edu.my/24742/ http://irep.iium.edu.my/24742/ http://irep.iium.edu.my/24742/1/Biotransformation_of_Malaysian_Catfish_Innova_Ceincia.pdf
first_indexed	2023-09-18T20:37:02Z
last_indexed	2023-09-18T20:37:02Z
_version_	1777409114656210944

Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa

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