Biotransformation of ω-fatty acid from Clarias gariepinus using Psuedomonas aeruginosa
Study on biotransformation of �-fatty acid from fish oil was carried out. Pseudomonas aeruginosa has been chosen to be the biocatalyst for biotransformation of w fatty acids extracted from Malaysian catfish, Clarias gariepinus. The lipid from freeze-dried catfish flesh was extracted using a modifi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academic Information Press
2012
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/24742/ http://irep.iium.edu.my/24742/ http://irep.iium.edu.my/24742/1/Biotransformation_of_Malaysian_Catfish_Innova_Ceincia.pdf |
| Summary: | Study on biotransformation of �-fatty acid from fish oil was carried out. Pseudomonas aeruginosa has been
chosen to be the biocatalyst for biotransformation of w fatty acids extracted from Malaysian catfish, Clarias
gariepinus. The lipid from freeze-dried catfish flesh was extracted using a modified Folch method and the
mixture of chloroform and methanol was used as a solvent. The crude lipid substrate was added to the bacterial
culture and incubated for 4 days. After conversion, the products were analyzed by using gas chromatography
and mass spectrometer (GC-MS). The analytical result showed that several fatty acids and cholesterol were
found in the product. However, the fatty acids and cholesterol contents before and after biotransformation were
different. The cholesterol content increased while �-fatty acid contents decreased after biotransformation
process. It can be concluded that the bacterial cells had oxidized the �-fatty acids to yield precursors which can
be utilized as starting materials for the bioconversion to cholesterol. |
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