Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare

Serum; a clear portion of blood obtained after removing cells, platelets and clotting factors is a universal supplement commonly used in media for mammalian cell culture system. Serum contains amino acids, proteins, growth factors, hormones, vitamins, inorganic substances, nutrients and metabolite...

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Main Authors:	Hashim, Yumi Zuhanis Has-Yun, Mel, Maizirwan, Mohd. Salleh, Hamzah, Ahmad Nor, Yusilawati, Othman, Siti Hajar, Wan Abdul Razak, Wan Yusra Hannanah
Format:	Article
Language:	English English
Published:	American-Eurasian Network for Scientific Information (AENSI) 2012
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/24535/ http://irep.iium.edu.my/24535/ http://irep.iium.edu.my/24535/1/Serum_in_mammalian_cell_culture-_weighing_the_challenges_of.pdf http://irep.iium.edu.my/24535/4/24535_Serum%20in%20mammalian%20cell%20culture_SCOPUS.pdf

id	iium-24535
recordtype	eprints
spelling	iium-245352019-01-14T01:19:20Z http://irep.iium.edu.my/24535/ Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare Hashim, Yumi Zuhanis Has-Yun Mel, Maizirwan Mohd. Salleh, Hamzah Ahmad Nor, Yusilawati Othman, Siti Hajar Wan Abdul Razak, Wan Yusra Hannanah Q Science (General) Serum; a clear portion of blood obtained after removing cells, platelets and clotting factors is a universal supplement commonly used in media for mammalian cell culture system. Serum contains amino acids, proteins, growth factors, hormones, vitamins, inorganic substances, nutrients and metabolites; which promote and sustain cell growth as well as provide buffering condition and protection to cells. Serum, from various animal sources such as bovine and porcine can be obtained commercially from manufacturers. Although serum has long been used successfully in mammalian cell culture system, recent trend is moving towards serum alternatives and serum-free media. This is due to many factors including the ethical and animal welfare issues in serum processing and production. Further, large scale production of bio-products using mammalian cell culture system prefers defined media which offers reproducibility and ease of downstream purification. Nevertheless, development of serum-free media is tedious and somewhat involve high cost with inherent problem of low growth rate as compared to serum supplemented media. This review is set to explore the many facets of serum and serum-free media in mammalian cell culture bioprocessing. American-Eurasian Network for Scientific Information (AENSI) 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/24535/1/Serum_in_mammalian_cell_culture-_weighing_the_challenges_of.pdf application/pdf en http://irep.iium.edu.my/24535/4/24535_Serum%20in%20mammalian%20cell%20culture_SCOPUS.pdf Hashim, Yumi Zuhanis Has-Yun and Mel, Maizirwan and Mohd. Salleh, Hamzah and Ahmad Nor, Yusilawati and Othman, Siti Hajar and Wan Abdul Razak, Wan Yusra Hannanah (2012) Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare. Advances in Natural and Applied Sciences, 6 (5). pp. 596-600. ISSN 1995-0772 http://www.aensiweb.com/anas/2012/596-600.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Hashim, Yumi Zuhanis Has-Yun Mel, Maizirwan Mohd. Salleh, Hamzah Ahmad Nor, Yusilawati Othman, Siti Hajar Wan Abdul Razak, Wan Yusra Hannanah Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare
description	Serum; a clear portion of blood obtained after removing cells, platelets and clotting factors is a universal supplement commonly used in media for mammalian cell culture system. Serum contains amino acids, proteins, growth factors, hormones, vitamins, inorganic substances, nutrients and metabolites; which promote and sustain cell growth as well as provide buffering condition and protection to cells. Serum, from various animal sources such as bovine and porcine can be obtained commercially from manufacturers. Although serum has long been used successfully in mammalian cell culture system, recent trend is moving towards serum alternatives and serum-free media. This is due to many factors including the ethical and animal welfare issues in serum processing and production. Further, large scale production of bio-products using mammalian cell culture system prefers defined media which offers reproducibility and ease of downstream purification. Nevertheless, development of serum-free media is tedious and somewhat involve high cost with inherent problem of low growth rate as compared to serum supplemented media. This review is set to explore the many facets of serum and serum-free media in mammalian cell culture bioprocessing.
format	Article
author	Hashim, Yumi Zuhanis Has-Yun Mel, Maizirwan Mohd. Salleh, Hamzah Ahmad Nor, Yusilawati Othman, Siti Hajar Wan Abdul Razak, Wan Yusra Hannanah
author_facet	Hashim, Yumi Zuhanis Has-Yun Mel, Maizirwan Mohd. Salleh, Hamzah Ahmad Nor, Yusilawati Othman, Siti Hajar Wan Abdul Razak, Wan Yusra Hannanah
author_sort	Hashim, Yumi Zuhanis Has-Yun
title	Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare
title_short	Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare
title_full	Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare
title_fullStr	Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare
title_full_unstemmed	Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare
title_sort	serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare
publisher	American-Eurasian Network for Scientific Information (AENSI)
publishDate	2012
url	http://irep.iium.edu.my/24535/ http://irep.iium.edu.my/24535/ http://irep.iium.edu.my/24535/1/Serum_in_mammalian_cell_culture-_weighing_the_challenges_of.pdf http://irep.iium.edu.my/24535/4/24535_Serum%20in%20mammalian%20cell%20culture_SCOPUS.pdf
first_indexed	2023-09-18T20:36:47Z
last_indexed	2023-09-18T20:36:47Z
_version_	1777409098751410176

Serum in mammalian cell culture: weighing the challenges of bioprocessing, ethics and animal welfare

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