Optimization of extraction process parameters for anti-cancer agent from Solanum Lycopersicum
In the fight against cancer, novel chemotherapeutic agents are continually being sought to complement existing drugs. The optimize extraction process is required to ensure that the maximum yield of anti-cancer agents will be extracted. In this study, three extraction parameters were investigated...
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| Format: | Book Chapter |
| Language: | English |
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IIUM Press
2011
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| Online Access: | http://irep.iium.edu.my/24484/ http://irep.iium.edu.my/24484/1/optimization_of_extraction_-_C1.pdf |
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iium-244842012-07-03T06:13:06Z http://irep.iium.edu.my/24484/ Optimization of extraction process parameters for anti-cancer agent from Solanum Lycopersicum Amid, Azura Ahmad, Abdul Aziz Ahmad Raus, Raha TP248.13 Biotechnology In the fight against cancer, novel chemotherapeutic agents are continually being sought to complement existing drugs. The optimize extraction process is required to ensure that the maximum yield of anti-cancer agents will be extracted. In this study, three extraction parameters were investigated on their effects toward the anti-cancer activities when applied to MCF-7 breast cancer cell line. A three-factor and three levels Box Behnken design consisting of seventeen experiments was employed including five replicates at the center point. The response variable will be the absorbance reading at 490 nm in terms of percentage cell viability. The design variables were the extraction time (min), extraction agitation speed (rpm) and solvent composition (%, v/v water/methanol). The optimum extraction was found to be 82% methanol, 24 hours and 110 rpm. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/24484/1/optimization_of_extraction_-_C1.pdf Amid, Azura and Ahmad, Abdul Aziz and Ahmad Raus, Raha (2011) Optimization of extraction process parameters for anti-cancer agent from Solanum Lycopersicum. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 1-9. ISBN 9789674181444 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
TP248.13 Biotechnology |
| spellingShingle |
TP248.13 Biotechnology Amid, Azura Ahmad, Abdul Aziz Ahmad Raus, Raha Optimization of extraction process parameters for anti-cancer agent from Solanum Lycopersicum |
| description |
In the fight against cancer, novel chemotherapeutic agents are continually being
sought to complement existing drugs. The optimize extraction process is required to
ensure that the maximum yield of anti-cancer agents will be extracted. In this study, three
extraction parameters were investigated on their effects toward the anti-cancer activities
when applied to MCF-7 breast cancer cell line. A three-factor and three levels Box
Behnken design consisting of seventeen experiments was employed including five
replicates at the center point. The response variable will be the absorbance reading at 490
nm in terms of percentage cell viability. The design variables were the extraction time
(min), extraction agitation speed (rpm) and solvent composition (%, v/v water/methanol).
The optimum extraction was found to be 82% methanol, 24 hours and 110 rpm. |
| format |
Book Chapter |
| author |
Amid, Azura Ahmad, Abdul Aziz Ahmad Raus, Raha |
| author_facet |
Amid, Azura Ahmad, Abdul Aziz Ahmad Raus, Raha |
| author_sort |
Amid, Azura |
| title |
Optimization of extraction process parameters for anti-cancer agent from Solanum Lycopersicum |
| title_short |
Optimization of extraction process parameters for anti-cancer agent from Solanum Lycopersicum |
| title_full |
Optimization of extraction process parameters for anti-cancer agent from Solanum Lycopersicum |
| title_fullStr |
Optimization of extraction process parameters for anti-cancer agent from Solanum Lycopersicum |
| title_full_unstemmed |
Optimization of extraction process parameters for anti-cancer agent from Solanum Lycopersicum |
| title_sort |
optimization of extraction process parameters for anti-cancer agent from solanum lycopersicum |
| publisher |
IIUM Press |
| publishDate |
2011 |
| url |
http://irep.iium.edu.my/24484/ http://irep.iium.edu.my/24484/1/optimization_of_extraction_-_C1.pdf |
| first_indexed |
2023-09-18T20:36:42Z |
| last_indexed |
2023-09-18T20:36:42Z |
| _version_ |
1777409093911183360 |