Modeling the growth kinetics of Escherichia Coli fermentation in bioreactors
ABSTRACT The chapter focuses on the growth kinetics and modelling of recombinant Escherichia coli in fermentation processes. Experimental data for from four bioreactors were analyzed using Monod, Logistic and Contois models. A comparison between the models' predicted values of cell, substrat...
| Main Authors: | , , |
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| Format: | Book Chapter |
| Language: | English |
| Published: |
IIUM Press
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/24222/ http://irep.iium.edu.my/24222/ http://irep.iium.edu.my/24222/3/Modeling_the_Growth.pdf |
| Summary: | ABSTRACT
The chapter focuses on the growth kinetics and modelling of recombinant Escherichia coli in fermentation processes. Experimental data for from four bioreactors were analyzed using Monod, Logistic and Contois models. A comparison between the models' predicted values of cell, substrate and product (β-glucuronidase enzyme) was done with the kinetic parameters mmax, YX/S, Ks, k1, and k2 calculated from the experimental data. Substrate used for this modelling was glucose while the independent variables were peptone soy, sodium chloride and yeast extract. The Logistic model is found to describe the growth of E. coli in the fermentation process with the limited supply of glucose and the Monod and Contois models failed to represent the cell growth profiles.
Keywords: growth kinetics, modeling, recombinant E. coli, fermentation.
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