An In Vivo Implantation Study in New Zealand White Rabbits for Granular Hydroxyapatite

Introduction: We developed hydroxyapatite (HA) granules made from local raw materials (GranuMaS™), which have been fabricated using a novel method (Patent (Pending) No. PI 2004 0748). GranuMaS™ have been fully characterized using X-ray diffraction, scanning electron microscopy, energy dispersive x-r...

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Main Authors:	Zulkifly, Ahmad Hafiz, Khalid, Kamarul Ariffin, Aziz, Mohd Yusof, Mohamed Amin, Mohamed Azril, Awang, Mohd Shukrimi, Mohd Yusof, Nazri, Che Ahmad, Aminudin, Zakaria@Mohamad, Zamzuri, Fazan, Fazilah
Format:	Conference or Workshop Item
Language:	English
Published:	2008
Subjects:	RD701 Orthopedics TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/23964/ http://irep.iium.edu.my/23964/ http://irep.iium.edu.my/23964/1/Hafiz%2C_A%2C_Khalid%2C_K.A%2C_Yusof%2C_A%2C_Azril%2C_M.A%2C_Shukrimi%2C_A%2C_Nazri%2C_M.Y%2C_Aminudin%2C_C.A%2C_Zamzuri%2C_Z%2C_Fazan%2C_F._European_Cells_and_Materials._July_2008._Volume_16%2C_Suppl.2_pp55.pdf

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spelling	iium-239642015-03-11T06:30:21Z http://irep.iium.edu.my/23964/ An In Vivo Implantation Study in New Zealand White Rabbits for Granular Hydroxyapatite Zulkifly, Ahmad Hafiz Khalid, Kamarul Ariffin Aziz, Mohd Yusof Mohamed Amin, Mohamed Azril Awang, Mohd Shukrimi Mohd Yusof, Nazri Che Ahmad, Aminudin Zakaria@Mohamad, Zamzuri Fazan, Fazilah RD701 Orthopedics TA164 Bioengineering Introduction: We developed hydroxyapatite (HA) granules made from local raw materials (GranuMaS™), which have been fabricated using a novel method (Patent (Pending) No. PI 2004 0748). GranuMaS™ have been fully characterized using X-ray diffraction, scanning electron microscopy, energy dispersive x-ray, Fourier transform infra-red spectroscopy and inductively coupled plasma methods. It has also been shown to comply with the ASTM F1185 - 88 (1993) specification standards. Biocompatibility studies have been performed on the product, which include neutral red cytotoxicity assay using cultured fibroblast and CRL-1427 osteoblast cells; MTT (Tetrazolium Salt) assay; cytotoxicity studies for apoptosis using Acridine Orange / Propidium Iodide (AO/PI) dual staining of V79 cells in DMEM; and COMET assay genotoxicity studies using L-929 (normal mouse epithelial) cells. An in vivo implantation study involving New Zealand White rabbits were carried out in the course of this study. Materials and Methods: HA granules of between 250 to 500 μm in diameter were implanted into a 9mm by 4.5mm defect made in the proximal metaphyseal region of the rabbits’ left tibia. The tibia was harvested at 2, 3, 4, 6 and 12 weeks. The retrieved specimens were processed to produce undecalcified tissue sections using a hard tissue band cutting and microgrinding system (EXAKT Apparatebau, Germany). Prepared slides were analyzed under the light microscope using compound polarized light, Toulidine Blue, Masson Goldner’s Trichrome and von Kossa stains. Results and Discussion: New bone formation is seen even at 2 weeks post-implantation, which further consolidates at 3, 4 and 6 weeks. At 12 weeks the new bone between the granules are well-formed and the cortical defects made have closed off with well-formed lamellar bone seen between the granules. There is direct apposition of the new bone to the granules. The new bone seen with bridging of the medullary cavity and no major voids or defects found. No evidence of any fibrous or inflammatory tissue was seen. Conclusions: GranuMaS™ is osteoconductive and biocompatible when implanted in rabbits. It could be used as an alternative bone graft substitute, as there is profound new bone formation in-between and around the granules. 2008-07 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/23964/1/Hafiz%2C_A%2C_Khalid%2C_K.A%2C_Yusof%2C_A%2C_Azril%2C_M.A%2C_Shukrimi%2C_A%2C_Nazri%2C_M.Y%2C_Aminudin%2C_C.A%2C_Zamzuri%2C_Z%2C_Fazan%2C_F._European_Cells_and_Materials._July_2008._Volume_16%2C_Suppl.2_pp55.pdf Zulkifly, Ahmad Hafiz and Khalid, Kamarul Ariffin and Aziz, Mohd Yusof and Mohamed Amin, Mohamed Azril and Awang, Mohd Shukrimi and Mohd Yusof, Nazri and Che Ahmad, Aminudin and Zakaria@Mohamad, Zamzuri and Fazan, Fazilah (2008) An In Vivo Implantation Study in New Zealand White Rabbits for Granular Hydroxyapatite. In: eCM IX MUSCULOSKELETAL TRAUMA: 50 YEARS OF AO RESEARCH, June 15-18, 2008, Convention Centre, Davos, Switzerland. http://www.ecmjournal.org/journal/supplements/vol016supp02/vol016supp02.htm
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RD701 Orthopedics TA164 Bioengineering
spellingShingle	RD701 Orthopedics TA164 Bioengineering Zulkifly, Ahmad Hafiz Khalid, Kamarul Ariffin Aziz, Mohd Yusof Mohamed Amin, Mohamed Azril Awang, Mohd Shukrimi Mohd Yusof, Nazri Che Ahmad, Aminudin Zakaria@Mohamad, Zamzuri Fazan, Fazilah An In Vivo Implantation Study in New Zealand White Rabbits for Granular Hydroxyapatite
description	Introduction: We developed hydroxyapatite (HA) granules made from local raw materials (GranuMaS™), which have been fabricated using a novel method (Patent (Pending) No. PI 2004 0748). GranuMaS™ have been fully characterized using X-ray diffraction, scanning electron microscopy, energy dispersive x-ray, Fourier transform infra-red spectroscopy and inductively coupled plasma methods. It has also been shown to comply with the ASTM F1185 - 88 (1993) specification standards. Biocompatibility studies have been performed on the product, which include neutral red cytotoxicity assay using cultured fibroblast and CRL-1427 osteoblast cells; MTT (Tetrazolium Salt) assay; cytotoxicity studies for apoptosis using Acridine Orange / Propidium Iodide (AO/PI) dual staining of V79 cells in DMEM; and COMET assay genotoxicity studies using L-929 (normal mouse epithelial) cells. An in vivo implantation study involving New Zealand White rabbits were carried out in the course of this study. Materials and Methods: HA granules of between 250 to 500 μm in diameter were implanted into a 9mm by 4.5mm defect made in the proximal metaphyseal region of the rabbits’ left tibia. The tibia was harvested at 2, 3, 4, 6 and 12 weeks. The retrieved specimens were processed to produce undecalcified tissue sections using a hard tissue band cutting and microgrinding system (EXAKT Apparatebau, Germany). Prepared slides were analyzed under the light microscope using compound polarized light, Toulidine Blue, Masson Goldner’s Trichrome and von Kossa stains. Results and Discussion: New bone formation is seen even at 2 weeks post-implantation, which further consolidates at 3, 4 and 6 weeks. At 12 weeks the new bone between the granules are well-formed and the cortical defects made have closed off with well-formed lamellar bone seen between the granules. There is direct apposition of the new bone to the granules. The new bone seen with bridging of the medullary cavity and no major voids or defects found. No evidence of any fibrous or inflammatory tissue was seen. Conclusions: GranuMaS™ is osteoconductive and biocompatible when implanted in rabbits. It could be used as an alternative bone graft substitute, as there is profound new bone formation in-between and around the granules.
format	Conference or Workshop Item
author	Zulkifly, Ahmad Hafiz Khalid, Kamarul Ariffin Aziz, Mohd Yusof Mohamed Amin, Mohamed Azril Awang, Mohd Shukrimi Mohd Yusof, Nazri Che Ahmad, Aminudin Zakaria@Mohamad, Zamzuri Fazan, Fazilah
author_facet	Zulkifly, Ahmad Hafiz Khalid, Kamarul Ariffin Aziz, Mohd Yusof Mohamed Amin, Mohamed Azril Awang, Mohd Shukrimi Mohd Yusof, Nazri Che Ahmad, Aminudin Zakaria@Mohamad, Zamzuri Fazan, Fazilah
author_sort	Zulkifly, Ahmad Hafiz
title	An In Vivo Implantation Study in New Zealand White Rabbits for Granular Hydroxyapatite
title_short	An In Vivo Implantation Study in New Zealand White Rabbits for Granular Hydroxyapatite
title_full	An In Vivo Implantation Study in New Zealand White Rabbits for Granular Hydroxyapatite
title_fullStr	An In Vivo Implantation Study in New Zealand White Rabbits for Granular Hydroxyapatite
title_full_unstemmed	An In Vivo Implantation Study in New Zealand White Rabbits for Granular Hydroxyapatite
title_sort	in vivo implantation study in new zealand white rabbits for granular hydroxyapatite
publishDate	2008
url	http://irep.iium.edu.my/23964/ http://irep.iium.edu.my/23964/ http://irep.iium.edu.my/23964/1/Hafiz%2C_A%2C_Khalid%2C_K.A%2C_Yusof%2C_A%2C_Azril%2C_M.A%2C_Shukrimi%2C_A%2C_Nazri%2C_M.Y%2C_Aminudin%2C_C.A%2C_Zamzuri%2C_Z%2C_Fazan%2C_F._European_Cells_and_Materials._July_2008._Volume_16%2C_Suppl.2_pp55.pdf
first_indexed	2023-09-18T20:36:05Z
last_indexed	2023-09-18T20:36:05Z
_version_	1777409054984896512

An In Vivo Implantation Study in New Zealand White Rabbits for Granular Hydroxyapatite

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