Anti-cancer activities of Thymoquinone, constituents of Nigella sativa against oral cancer cell lines

Objectives: The aims of this study were to investigate and determine anti-cancer potential of Thymoquinone (TQ), a major component of Nigella Sativa seeds against cell lines derived from oral cavity carcinoma. Methods: Three human cancer cell lines derived from oral cavity, Ca922, HSC-2 and HSC-3...

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Main Authors:	Arief Ichwan, Solachuddin Jauhari, Taher, Muhammad, Masa-Aki , Ikeda, Z. L, Abdullah, M.T, Junaidi
Format:	Conference or Workshop Item
Language:	English English
Published:	2010
Subjects:	RK Dentistry RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/23822/ http://irep.iium.edu.my/23822/ http://irep.iium.edu.my/23822/1/2010-iadr-abstract-form.pdf http://irep.iium.edu.my/23822/4/%21ADR_9th.pdf

id	iium-23822
recordtype	eprints
spelling	iium-238222013-03-21T06:31:05Z http://irep.iium.edu.my/23822/ Anti-cancer activities of Thymoquinone, constituents of Nigella sativa against oral cancer cell lines Arief Ichwan, Solachuddin Jauhari Taher, Muhammad Masa-Aki , Ikeda Z. L, Abdullah M.T, Junaidi RK Dentistry RM300 Drugs and their action Objectives: The aims of this study were to investigate and determine anti-cancer potential of Thymoquinone (TQ), a major component of Nigella Sativa seeds against cell lines derived from oral cavity carcinoma. Methods: Three human cancer cell lines derived from oral cavity, Ca922, HSC-2 and HSC-3 were used. HCT116 (human colorectal carcinoma) cells were also used as the positive control. The cells were exposed to TQ solution at different concentrations and then analyzed after certain culture periods (12 and 24 hours). Cytotoxic activity was quantified by MTT assay. Apoptosis (programmed cell death) induction was detected by DNA Fragmentation assay. Wound-healing migration assay (Scratch assay) was performed to determine cells migration. Results: TQ dose- and time-dependently reduced the viability of Ca922, HSC-2 and HSC-3 cells. TQ treatment at 20 μM was sufficient to inhibit cancer cell migration after 12 hours. Moreover, DNA fragmentation assay shows that TQ treatment induced apoptosis in all of cell lines tested. Conclusions: TQ exhibits anti-cancer activities against oral cancer cell lines. This study suggests the potential benefit of TQ as a natural chemotherapeutic agent for oral cancer treatment. 2010 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/23822/1/2010-iadr-abstract-form.pdf application/pdf en http://irep.iium.edu.my/23822/4/%21ADR_9th.pdf Arief Ichwan, Solachuddin Jauhari and Taher, Muhammad and Masa-Aki , Ikeda and Z. L, Abdullah and M.T, Junaidi (2010) Anti-cancer activities of Thymoquinone, constituents of Nigella sativa against oral cancer cell lines. In: 9th Scientific Meeting of International Association for Dental Reesarch, 5-6 February 2010, UKM, Kuala Lumpur. (Unpublished) http://www.iadr.org/i4a/pages/index.cfm?pageid=3777
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RK Dentistry RM300 Drugs and their action
spellingShingle	RK Dentistry RM300 Drugs and their action Arief Ichwan, Solachuddin Jauhari Taher, Muhammad Masa-Aki , Ikeda Z. L, Abdullah M.T, Junaidi Anti-cancer activities of Thymoquinone, constituents of Nigella sativa against oral cancer cell lines
description	Objectives: The aims of this study were to investigate and determine anti-cancer potential of Thymoquinone (TQ), a major component of Nigella Sativa seeds against cell lines derived from oral cavity carcinoma. Methods: Three human cancer cell lines derived from oral cavity, Ca922, HSC-2 and HSC-3 were used. HCT116 (human colorectal carcinoma) cells were also used as the positive control. The cells were exposed to TQ solution at different concentrations and then analyzed after certain culture periods (12 and 24 hours). Cytotoxic activity was quantified by MTT assay. Apoptosis (programmed cell death) induction was detected by DNA Fragmentation assay. Wound-healing migration assay (Scratch assay) was performed to determine cells migration. Results: TQ dose- and time-dependently reduced the viability of Ca922, HSC-2 and HSC-3 cells. TQ treatment at 20 μM was sufficient to inhibit cancer cell migration after 12 hours. Moreover, DNA fragmentation assay shows that TQ treatment induced apoptosis in all of cell lines tested. Conclusions: TQ exhibits anti-cancer activities against oral cancer cell lines. This study suggests the potential benefit of TQ as a natural chemotherapeutic agent for oral cancer treatment.
format	Conference or Workshop Item
author	Arief Ichwan, Solachuddin Jauhari Taher, Muhammad Masa-Aki , Ikeda Z. L, Abdullah M.T, Junaidi
author_facet	Arief Ichwan, Solachuddin Jauhari Taher, Muhammad Masa-Aki , Ikeda Z. L, Abdullah M.T, Junaidi
author_sort	Arief Ichwan, Solachuddin Jauhari
title	Anti-cancer activities of Thymoquinone, constituents of Nigella sativa against oral cancer cell lines
title_short	Anti-cancer activities of Thymoquinone, constituents of Nigella sativa against oral cancer cell lines
title_full	Anti-cancer activities of Thymoquinone, constituents of Nigella sativa against oral cancer cell lines
title_fullStr	Anti-cancer activities of Thymoquinone, constituents of Nigella sativa against oral cancer cell lines
title_full_unstemmed	Anti-cancer activities of Thymoquinone, constituents of Nigella sativa against oral cancer cell lines
title_sort	anti-cancer activities of thymoquinone, constituents of nigella sativa against oral cancer cell lines
publishDate	2010
url	http://irep.iium.edu.my/23822/ http://irep.iium.edu.my/23822/ http://irep.iium.edu.my/23822/1/2010-iadr-abstract-form.pdf http://irep.iium.edu.my/23822/4/%21ADR_9th.pdf
first_indexed	2023-09-18T20:35:58Z
last_indexed	2023-09-18T20:35:58Z
_version_	1777409047651155968

Anti-cancer activities of Thymoquinone, constituents of Nigella sativa against oral cancer cell lines

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