Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture

In aquaculture probiotic feeding could play a crucial role in developing microbial control strategies, since disease outbreaks are recognized as important constraints to aquaculture production and the fear of antibiotic resistance. In this study, lactic acid bacteria (LAB) strains from the intestina...

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Main Authors:	Tengku Abdul Hamid, Tengku Haziyamin, Khan Chowdhury, Ahmed Jalal, Jalil, Muhammad Fauzi, Azhar, Nur Shazana
Format:	Article
Language:	English
Published:	Academic Journals 2012
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/23417/ http://irep.iium.edu.my/23417/ http://irep.iium.edu.my/23417/ http://irep.iium.edu.my/23417/1/African-catfish_Hamid_et_al.pdf

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recordtype	eprints
spelling	iium-234172017-12-08T02:27:41Z http://irep.iium.edu.my/23417/ Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture Tengku Abdul Hamid, Tengku Haziyamin Khan Chowdhury, Ahmed Jalal Jalil, Muhammad Fauzi Azhar, Nur Shazana Q Science (General) In aquaculture probiotic feeding could play a crucial role in developing microbial control strategies, since disease outbreaks are recognized as important constraints to aquaculture production and the fear of antibiotic resistance. In this study, lactic acid bacteria (LAB) strains from the intestinal tissue of African catfish Clarias griepinus were successfully screened, characterized and identified. The isolates (S1#8, S1#9, S1#10, S1#18, S1#19 S1#20 and S1#21) shared common morphological characteristics of LAB such as non-sporulating, Gram positive cocci or cocco-bacilli shape. These were the dominating morphology found in the catfish, as compared to other types of LABs reported to be found in freshwater fishes. All of the 35 isolates have the ability to utilize lactose as part of their metabolism process and showed negative reactions towards catalase test. These isolates were also tested for antimicrobial activities using disc diffusion assay against indicator Salmonella typhimurium and Escherichia coli. Based on the partial 16S rRNA sequences, the selected LAB isolates belonged to a member of Lactococcus lactis with 98% DNA similarity. This strain can be used as probiotic in aquaculture feeding. Academic Journals 2012-04-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/23417/1/African-catfish_Hamid_et_al.pdf Tengku Abdul Hamid, Tengku Haziyamin and Khan Chowdhury, Ahmed Jalal and Jalil, Muhammad Fauzi and Azhar, Nur Shazana (2012) Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture. African Journal of Biotechnology, 11 (29). pp. 7494-7499. ISSN 1684–5315 http://academicjournals.org/ajb/abstracts/abs2012/10Apr/Hamid%20et%20al.htm 10.5897/AJB11.3864
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Tengku Abdul Hamid, Tengku Haziyamin Khan Chowdhury, Ahmed Jalal Jalil, Muhammad Fauzi Azhar, Nur Shazana Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture
description	In aquaculture probiotic feeding could play a crucial role in developing microbial control strategies, since disease outbreaks are recognized as important constraints to aquaculture production and the fear of antibiotic resistance. In this study, lactic acid bacteria (LAB) strains from the intestinal tissue of African catfish Clarias griepinus were successfully screened, characterized and identified. The isolates (S1#8, S1#9, S1#10, S1#18, S1#19 S1#20 and S1#21) shared common morphological characteristics of LAB such as non-sporulating, Gram positive cocci or cocco-bacilli shape. These were the dominating morphology found in the catfish, as compared to other types of LABs reported to be found in freshwater fishes. All of the 35 isolates have the ability to utilize lactose as part of their metabolism process and showed negative reactions towards catalase test. These isolates were also tested for antimicrobial activities using disc diffusion assay against indicator Salmonella typhimurium and Escherichia coli. Based on the partial 16S rRNA sequences, the selected LAB isolates belonged to a member of Lactococcus lactis with 98% DNA similarity. This strain can be used as probiotic in aquaculture feeding.
format	Article
author	Tengku Abdul Hamid, Tengku Haziyamin Khan Chowdhury, Ahmed Jalal Jalil, Muhammad Fauzi Azhar, Nur Shazana
author_facet	Tengku Abdul Hamid, Tengku Haziyamin Khan Chowdhury, Ahmed Jalal Jalil, Muhammad Fauzi Azhar, Nur Shazana
author_sort	Tengku Abdul Hamid, Tengku Haziyamin
title	Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture
title_short	Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture
title_full	Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture
title_fullStr	Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture
title_full_unstemmed	Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture
title_sort	isolation and screening of lactic acid bacteria, lactococcus lactis from clarias gariepinus (african catfish) with potential use as probiotic in aquaculture
publisher	Academic Journals
publishDate	2012
url	http://irep.iium.edu.my/23417/ http://irep.iium.edu.my/23417/ http://irep.iium.edu.my/23417/ http://irep.iium.edu.my/23417/1/African-catfish_Hamid_et_al.pdf
first_indexed	2023-09-18T20:35:26Z
last_indexed	2023-09-18T20:35:26Z
_version_	1777409013983477760

Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture

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