Gas chromatography - mass spectrometry (GC-MS) based metabolic fingerprinting of three Malaysia ginger (zingiber officinale rosc.) cultivars

Gas chromatography - mass spectrometry (GC-MS) based metabolic fingerprinting of three Malaysia ginger (zingiber officinale rosc.) cultivars

A comprehensive metabolic fingerprinting of three micro propagated ginger explants, Bukit Tinggi, Tanjung Sepat and Sabah cultivars, was carried out using Gas chromatography coupled with mass spectrometry (GC-MS). The ginger leave tissues were fractionated in a polar (MeOH) and non polar (CHCl...

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Main Authors: Budi Muljono, Julia Retno Andayani, Ishak, Mahdi
Format: Conference or Workshop Item
Language:English
Published: 2010
Subjects:
TP Chemical technology
Online Access:http://irep.iium.edu.my/23115/
http://irep.iium.edu.my/23115/
http://irep.iium.edu.my/23115/1/p59.pdf
id iium-23115
recordtype eprints
spelling iium-231152013-07-18T00:59:59Z http://irep.iium.edu.my/23115/ Gas chromatography - mass spectrometry (GC-MS) based metabolic fingerprinting of three Malaysia ginger (zingiber officinale rosc.) cultivars Budi Muljono, Julia Retno Andayani Ishak, Mahdi TP Chemical technology A comprehensive metabolic fingerprinting of three micro propagated ginger explants, Bukit Tinggi, Tanjung Sepat and Sabah cultivars, was carried out using Gas chromatography coupled with mass spectrometry (GC-MS). The ginger leave tissues were fractionated in a polar (MeOH) and non polar (CHCl3) solvents, subsequently methoximated and silylated prior to GC-MS analysis. By applying this technique, over 300 metabolites (polar and non-polar) in total were detected in each ginger cultivar. However, only about 25% of these compounds can be definitely characterised by using the Wiley7n.1 and the NIST Mass spectra libraries for the best hit of the molecular ion peaks and the fragmentation patterns. Fatty acids and sugars (mono− and disaccharides) as the main constituents of the ginger leaf tissues besides a small amount of essential amino acids as well as some organic acids. In addition, a distinct GCMS metabolic fingerprinting in each of the ginger cultivar can be used as “unequivocal pattern recognition” among the ginger phenotype derived from Bukit Tinggi, Tanjung Sepat and Sabah. 2010 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/23115/1/p59.pdf Budi Muljono, Julia Retno Andayani and Ishak, Mahdi (2010) Gas chromatography - mass spectrometry (GC-MS) based metabolic fingerprinting of three Malaysia ginger (zingiber officinale rosc.) cultivars. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. http://www.iium.edu.my/irie/10/sub10/author/list_p.php
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Budi Muljono, Julia Retno Andayani
Ishak, Mahdi
Gas chromatography - mass spectrometry (GC-MS) based metabolic fingerprinting of three Malaysia ginger (zingiber officinale rosc.) cultivars
description A comprehensive metabolic fingerprinting of three micro propagated ginger explants, Bukit Tinggi, Tanjung Sepat and Sabah cultivars, was carried out using Gas chromatography coupled with mass spectrometry (GC-MS). The ginger leave tissues were fractionated in a polar (MeOH) and non polar (CHCl3) solvents, subsequently methoximated and silylated prior to GC-MS analysis. By applying this technique, over 300 metabolites (polar and non-polar) in total were detected in each ginger cultivar. However, only about 25% of these compounds can be definitely characterised by using the Wiley7n.1 and the NIST Mass spectra libraries for the best hit of the molecular ion peaks and the fragmentation patterns. Fatty acids and sugars (mono− and disaccharides) as the main constituents of the ginger leaf tissues besides a small amount of essential amino acids as well as some organic acids. In addition, a distinct GCMS metabolic fingerprinting in each of the ginger cultivar can be used as “unequivocal pattern recognition” among the ginger phenotype derived from Bukit Tinggi, Tanjung Sepat and Sabah.
format Conference or Workshop Item
author Budi Muljono, Julia Retno Andayani
Ishak, Mahdi
author_facet Budi Muljono, Julia Retno Andayani
Ishak, Mahdi
author_sort Budi Muljono, Julia Retno Andayani
title Gas chromatography - mass spectrometry (GC-MS) based metabolic fingerprinting of three Malaysia ginger (zingiber officinale rosc.) cultivars
title_short Gas chromatography - mass spectrometry (GC-MS) based metabolic fingerprinting of three Malaysia ginger (zingiber officinale rosc.) cultivars
title_full Gas chromatography - mass spectrometry (GC-MS) based metabolic fingerprinting of three Malaysia ginger (zingiber officinale rosc.) cultivars
title_fullStr Gas chromatography - mass spectrometry (GC-MS) based metabolic fingerprinting of three Malaysia ginger (zingiber officinale rosc.) cultivars
title_full_unstemmed Gas chromatography - mass spectrometry (GC-MS) based metabolic fingerprinting of three Malaysia ginger (zingiber officinale rosc.) cultivars
title_sort gas chromatography - mass spectrometry (gc-ms) based metabolic fingerprinting of three malaysia ginger (zingiber officinale rosc.) cultivars
publishDate 2010
url http://irep.iium.edu.my/23115/
http://irep.iium.edu.my/23115/
http://irep.iium.edu.my/23115/1/p59.pdf
first_indexed 2023-09-18T20:35:03Z
last_indexed 2023-09-18T20:35:03Z
_version_ 1777408989781295104

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