Immobilization of cellulases enzyme on Carbon Nanotubes (CNTs) for cellulosic compounds degradation

The fast growing palm oil industry in Malaysia generates, amongst other wastes, Empty Fruit Bunch (EFB) which consists of cellulosic materials. It is one of the major sources of Greenhouse Gases (GHG). However, the bioconversion of cellulosic materials in EFB, a renewable biomass, to valuable pr...

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Main Authors:	Al-Khatib, Ma An Fahmi Rashid, Alam, Md. Zahangir, Mohammed Abd, Rasha
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	TA401 Materials of engineering and construction
Online Access:	http://irep.iium.edu.my/23101/ http://irep.iium.edu.my/23101/ http://irep.iium.edu.my/23101/1/pp41.pdf

id	iium-23101
recordtype	eprints
spelling	iium-231012013-06-19T01:39:46Z http://irep.iium.edu.my/23101/ Immobilization of cellulases enzyme on Carbon Nanotubes (CNTs) for cellulosic compounds degradation Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Mohammed Abd, Rasha TA401 Materials of engineering and construction The fast growing palm oil industry in Malaysia generates, amongst other wastes, Empty Fruit Bunch (EFB) which consists of cellulosic materials. It is one of the major sources of Greenhouse Gases (GHG). However, the bioconversion of cellulosic materials in EFB, a renewable biomass, to valuable products will be the solution to the disposal problem and hence minimize the pollution. The bioconversion of cellulosic materials is carried out by using cellulase enzyme, which itself was extracted from sludge, immobilized on functionalized carbon nanotubes (CNTs) in the presence of coupling reagent. The process parameters such as reaction temperature, reaction time, pH, and amount of enzyme, CNTs dosage and EDC were optimized by using design expert software. The morphology and the structure of CNTs were characterized by Field Emission Scanning Electron Microscopy (FSEM) and Fourier Transform Infrared Absorption Spectroscopic (FTIR). Firstly, carbon nanotubes were functionalized by acidic treatment. Then, the cellulase enzyme is immobilized on the functionalized (CNTs) in a solution of Nhydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylamino propyl)-carbodiimide hydrochloride (EDC). The amount of enzyme attached on (CNTs) will be measured through UV spectrometer to determine the cellulases catalytic activity after immobilization and compare it with the free enzyme. Finally, the immobilized enzyme will be tested in the degradation of cellulosic material of empty fruit bunch (EFB) from palm oil mill effluent. 2010 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/23101/1/pp41.pdf Al-Khatib, Ma An Fahmi Rashid and Alam, Md. Zahangir and Mohammed Abd, Rasha (2010) Immobilization of cellulases enzyme on Carbon Nanotubes (CNTs) for cellulosic compounds degradation. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. http://www.iium.edu.my/irie/10/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA401 Materials of engineering and construction
spellingShingle	TA401 Materials of engineering and construction Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Mohammed Abd, Rasha Immobilization of cellulases enzyme on Carbon Nanotubes (CNTs) for cellulosic compounds degradation
description	The fast growing palm oil industry in Malaysia generates, amongst other wastes, Empty Fruit Bunch (EFB) which consists of cellulosic materials. It is one of the major sources of Greenhouse Gases (GHG). However, the bioconversion of cellulosic materials in EFB, a renewable biomass, to valuable products will be the solution to the disposal problem and hence minimize the pollution. The bioconversion of cellulosic materials is carried out by using cellulase enzyme, which itself was extracted from sludge, immobilized on functionalized carbon nanotubes (CNTs) in the presence of coupling reagent. The process parameters such as reaction temperature, reaction time, pH, and amount of enzyme, CNTs dosage and EDC were optimized by using design expert software. The morphology and the structure of CNTs were characterized by Field Emission Scanning Electron Microscopy (FSEM) and Fourier Transform Infrared Absorption Spectroscopic (FTIR). Firstly, carbon nanotubes were functionalized by acidic treatment. Then, the cellulase enzyme is immobilized on the functionalized (CNTs) in a solution of Nhydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylamino propyl)-carbodiimide hydrochloride (EDC). The amount of enzyme attached on (CNTs) will be measured through UV spectrometer to determine the cellulases catalytic activity after immobilization and compare it with the free enzyme. Finally, the immobilized enzyme will be tested in the degradation of cellulosic material of empty fruit bunch (EFB) from palm oil mill effluent.
format	Conference or Workshop Item
author	Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Mohammed Abd, Rasha
author_facet	Al-Khatib, Ma An Fahmi Rashid Alam, Md. Zahangir Mohammed Abd, Rasha
author_sort	Al-Khatib, Ma An Fahmi Rashid
title	Immobilization of cellulases enzyme on Carbon Nanotubes (CNTs) for cellulosic compounds degradation
title_short	Immobilization of cellulases enzyme on Carbon Nanotubes (CNTs) for cellulosic compounds degradation
title_full	Immobilization of cellulases enzyme on Carbon Nanotubes (CNTs) for cellulosic compounds degradation
title_fullStr	Immobilization of cellulases enzyme on Carbon Nanotubes (CNTs) for cellulosic compounds degradation
title_full_unstemmed	Immobilization of cellulases enzyme on Carbon Nanotubes (CNTs) for cellulosic compounds degradation
title_sort	immobilization of cellulases enzyme on carbon nanotubes (cnts) for cellulosic compounds degradation
publishDate	2010
url	http://irep.iium.edu.my/23101/ http://irep.iium.edu.my/23101/ http://irep.iium.edu.my/23101/1/pp41.pdf
first_indexed	2023-09-18T20:35:01Z
last_indexed	2023-09-18T20:35:01Z
_version_	1777408987897004032

Immobilization of cellulases enzyme on Carbon Nanotubes (CNTs) for cellulosic compounds degradation

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