Effects of menstrual cycle phase on metabolomic profiles in premenopausal women

Background: Characterization of the normal degree of physiological variation in the metabolomic profiles of healthy humans is a necessary step in the development of metabolomics as both a clinical research and diagnostic tool. This study investigated the effects of the menstrual cycle on 1H nuclear...

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Main Authors:	Wallace, M., Hashim, Yumi Zuhanis Has-Yun, Wingfield, M., Culliton, M., McAuliffe, F., Gibney, M. J., Brennan, L.
Format:	Article
Language:	English
Published:	Oxford University Press /The European Society of Human Reproduction and Embryology 2010
Subjects:	QP Physiology RG Gynecology and obstetrics
Online Access:	http://irep.iium.edu.my/2276/ http://irep.iium.edu.my/2276/ http://irep.iium.edu.my/2276/ http://irep.iium.edu.my/2276/4/Yumi_Zuhanis_Effects_of_menstrual_949.full.pdf

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recordtype	eprints
spelling	iium-22762013-07-22T04:02:18Z http://irep.iium.edu.my/2276/ Effects of menstrual cycle phase on metabolomic profiles in premenopausal women Wallace, M. Hashim, Yumi Zuhanis Has-Yun Wingfield, M. Culliton, M. McAuliffe, F. Gibney, M. J. Brennan, L. QP Physiology RG Gynecology and obstetrics Background: Characterization of the normal degree of physiological variation in the metabolomic profiles of healthy humans is a necessary step in the development of metabolomics as both a clinical research and diagnostic tool. This study investigated the effects of the menstrual cycle on 1H nuclear magnetic resonance (NMR) derived metabolomic profiles of urine and plasma from healthy women. Methods: In this study, 34 healthy women were recruited and a first void urine and fasting blood sample were collected from each woman at four different time points during one menstrual cycle. Serum hormone levels were used in combination with the menstrual calendar to classify the urine and plasma samples into five different phases i.e. menstrual, follicular, periovulatory, luteal and premenstrual. The urine and plasma samples were analysed using 1H NMR spectroscopy and subsequent data were analysed using principal component analysis (PCA) and partial least squares discriminant analysis. Results: PCA of the urine spectra showed no separation of samples based on the phases of the menstrual cycle. Multivariate analysis of the plasma spectra showed a separation of the menstrual phase and the luteal phase samples (R2 ¼ 0.61, Q2 ¼ 0.41). Subsequent analysis revealed a significant decrease in levels of glutamine, glycine, alanine, lysine, serine and creatinine and a significant increase in levels of acetoacetate and very low density lipoprotein (VLDL CH2) during the luteal phase. conclusions: These results establish a need to control for metabolic changes that occur in plasma due to the menstrual cycle in the design of future metabolomic studies involving premenopausal women. Oxford University Press /The European Society of Human Reproduction and Embryology 2010-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/2276/4/Yumi_Zuhanis_Effects_of_menstrual_949.full.pdf Wallace, M. and Hashim, Yumi Zuhanis Has-Yun and Wingfield, M. and Culliton, M. and McAuliffe, F. and Gibney, M. J. and Brennan, L. (2010) Effects of menstrual cycle phase on metabolomic profiles in premenopausal women. Human Reproductive, 25 (4). pp. 949-956. ISSN 1460-2350 http://humrep.oxfordjournals.org/ 10.1093/humrep/deq011
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QP Physiology RG Gynecology and obstetrics
spellingShingle	QP Physiology RG Gynecology and obstetrics Wallace, M. Hashim, Yumi Zuhanis Has-Yun Wingfield, M. Culliton, M. McAuliffe, F. Gibney, M. J. Brennan, L. Effects of menstrual cycle phase on metabolomic profiles in premenopausal women
description	Background: Characterization of the normal degree of physiological variation in the metabolomic profiles of healthy humans is a necessary step in the development of metabolomics as both a clinical research and diagnostic tool. This study investigated the effects of the menstrual cycle on 1H nuclear magnetic resonance (NMR) derived metabolomic profiles of urine and plasma from healthy women. Methods: In this study, 34 healthy women were recruited and a first void urine and fasting blood sample were collected from each woman at four different time points during one menstrual cycle. Serum hormone levels were used in combination with the menstrual calendar to classify the urine and plasma samples into five different phases i.e. menstrual, follicular, periovulatory, luteal and premenstrual. The urine and plasma samples were analysed using 1H NMR spectroscopy and subsequent data were analysed using principal component analysis (PCA) and partial least squares discriminant analysis. Results: PCA of the urine spectra showed no separation of samples based on the phases of the menstrual cycle. Multivariate analysis of the plasma spectra showed a separation of the menstrual phase and the luteal phase samples (R2 ¼ 0.61, Q2 ¼ 0.41). Subsequent analysis revealed a significant decrease in levels of glutamine, glycine, alanine, lysine, serine and creatinine and a significant increase in levels of acetoacetate and very low density lipoprotein (VLDL CH2) during the luteal phase. conclusions: These results establish a need to control for metabolic changes that occur in plasma due to the menstrual cycle in the design of future metabolomic studies involving premenopausal women.
format	Article
author	Wallace, M. Hashim, Yumi Zuhanis Has-Yun Wingfield, M. Culliton, M. McAuliffe, F. Gibney, M. J. Brennan, L.
author_facet	Wallace, M. Hashim, Yumi Zuhanis Has-Yun Wingfield, M. Culliton, M. McAuliffe, F. Gibney, M. J. Brennan, L.
author_sort	Wallace, M.
title	Effects of menstrual cycle phase on metabolomic profiles in premenopausal women
title_short	Effects of menstrual cycle phase on metabolomic profiles in premenopausal women
title_full	Effects of menstrual cycle phase on metabolomic profiles in premenopausal women
title_fullStr	Effects of menstrual cycle phase on metabolomic profiles in premenopausal women
title_full_unstemmed	Effects of menstrual cycle phase on metabolomic profiles in premenopausal women
title_sort	effects of menstrual cycle phase on metabolomic profiles in premenopausal women
publisher	Oxford University Press /The European Society of Human Reproduction and Embryology
publishDate	2010
url	http://irep.iium.edu.my/2276/ http://irep.iium.edu.my/2276/ http://irep.iium.edu.my/2276/ http://irep.iium.edu.my/2276/4/Yumi_Zuhanis_Effects_of_menstrual_949.full.pdf
first_indexed	2023-09-18T20:09:49Z
last_indexed	2023-09-18T20:09:49Z
_version_	1777407402398711808

Effects of menstrual cycle phase on metabolomic profiles in premenopausal women

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