Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement

Objectives Nerve growth factor (NGF) and its receptors, p75 and tyrosine receptor kinase A (Trk A), have been shown to increase following trauma. The aims of this study were to examine changes in the detection of NGF and its receptors during orthodontic tooth movement in the rat, and the effects of...

Full description

Bibliographic Details
Main Authors:	Hussin, Akbar Sham, Sampson, Wayne John, Dreyer, Craig William, Pierce, Angela Mary, Ferguson, Ian Andrew
Format:	Article
Language:	English
Published:	Pergamon 2009
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/22589/ http://irep.iium.edu.my/22589/ http://irep.iium.edu.my/22589/1/Dr_akbar.pdf

id	iium-22589
recordtype	eprints
spelling	iium-225892012-03-15T08:55:09Z http://irep.iium.edu.my/22589/ Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement Hussin, Akbar Sham Sampson, Wayne John Dreyer, Craig William Pierce, Angela Mary Ferguson, Ian Andrew RK Dentistry Objectives Nerve growth factor (NGF) and its receptors, p75 and tyrosine receptor kinase A (Trk A), have been shown to increase following trauma. The aims of this study were to examine changes in the detection of NGF and its receptors during orthodontic tooth movement in the rat, and the effects of anti-NGF on these changes. Design Orthodontic separators were placed between the right maxillary first and second molars of Sprague–Dawley rats which were equally divided into two groups. Animals from the second group were injected with anti-NGF. The left sides served as controls, and animals were sacrificed at 0, 3, 7 and 14 days. Results Results of immunohistochemical localisation for p75, Trk A, calcitonin gene-related peptide (CGRP) and NGF showed staining intensity increased at day 3, with a peak at day 7 and decreasing intensity at day 14. Anti-NGF injected animals showed reduced staining at all observation periods. Conclusion Data suggest that orthodontic injury induces NGF production, leading to sprouting and invasion by CGRP-positive nerve fibers and that injection of anti-NGF reduces NGF tissue levels and prevents innervation by CGRP-positive fibers. Pergamon 2009-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/22589/1/Dr_akbar.pdf Hussin, Akbar Sham and Sampson, Wayne John and Dreyer, Craig William and Pierce, Angela Mary and Ferguson, Ian Andrew (2009) Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement. Archives of Oral Biology, 54 (9). pp. 871-878. ISSN 0003-9969 http://www.elsevier.com/wps/find/journaldescription.cws_home/203/description#description
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Hussin, Akbar Sham Sampson, Wayne John Dreyer, Craig William Pierce, Angela Mary Ferguson, Ian Andrew Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement
description	Objectives Nerve growth factor (NGF) and its receptors, p75 and tyrosine receptor kinase A (Trk A), have been shown to increase following trauma. The aims of this study were to examine changes in the detection of NGF and its receptors during orthodontic tooth movement in the rat, and the effects of anti-NGF on these changes. Design Orthodontic separators were placed between the right maxillary first and second molars of Sprague–Dawley rats which were equally divided into two groups. Animals from the second group were injected with anti-NGF. The left sides served as controls, and animals were sacrificed at 0, 3, 7 and 14 days. Results Results of immunohistochemical localisation for p75, Trk A, calcitonin gene-related peptide (CGRP) and NGF showed staining intensity increased at day 3, with a peak at day 7 and decreasing intensity at day 14. Anti-NGF injected animals showed reduced staining at all observation periods. Conclusion Data suggest that orthodontic injury induces NGF production, leading to sprouting and invasion by CGRP-positive nerve fibers and that injection of anti-NGF reduces NGF tissue levels and prevents innervation by CGRP-positive fibers.
format	Article
author	Hussin, Akbar Sham Sampson, Wayne John Dreyer, Craig William Pierce, Angela Mary Ferguson, Ian Andrew
author_facet	Hussin, Akbar Sham Sampson, Wayne John Dreyer, Craig William Pierce, Angela Mary Ferguson, Ian Andrew
author_sort	Hussin, Akbar Sham
title	Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement
title_short	Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement
title_full	Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement
title_fullStr	Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement
title_full_unstemmed	Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement
title_sort	immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement
publisher	Pergamon
publishDate	2009
url	http://irep.iium.edu.my/22589/ http://irep.iium.edu.my/22589/ http://irep.iium.edu.my/22589/1/Dr_akbar.pdf
first_indexed	2023-09-18T20:34:23Z
last_indexed	2023-09-18T20:34:23Z
_version_	1777408947725008896

Immunohistochemical detection of nerve growth factor and its receptors in the rat periodontal ligament during tooth movement

Similar Items