Development of patatin-like protein purification method from natural rubber industrial waste

Skim latex is the byproduct of natural rubber processing. It is treated as effluent and lavishly left in acid pond treatment which creates a lot of environmental problems. The serum extracted from skim latex was found to contain patatin-like protein which is homologous to patatin storage protein...

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Main Authors:	Abdullah, Nurul Ain Harmiza, Yusof, Faridah, Mohd Nor, Zairossani
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/22564/ http://irep.iium.edu.my/22564/1/Development_of_Patatin-like_Protein_Purification_Method.pdf

id	iium-22564
recordtype	eprints
spelling	iium-225642012-07-06T00:55:35Z http://irep.iium.edu.my/22564/ Development of patatin-like protein purification method from natural rubber industrial waste Abdullah, Nurul Ain Harmiza Yusof, Faridah Mohd Nor, Zairossani TP248.13 Biotechnology Skim latex is the byproduct of natural rubber processing. It is treated as effluent and lavishly left in acid pond treatment which creates a lot of environmental problems. The serum extracted from skim latex was found to contain patatin-like protein which is homologous to patatin storage proteins in potato and tomato. Patatin belongs to a group of plant storage glycoproteins that show lipid acyl hydrolase activity which may be a means of defense against plant parasites. A definite method to purify patatin-like protein from rubber waste with minimal loss of target protein is therefore desirable. Three different methods of column chromatography were employed in the purification process to obtain the purified target protein. The success of this protein purification technique was assayed by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, lipid acyl hydrolase activity analysis and Bradford determination of protein concentration to quantify the protein yield. Through this method, the patatin-like protein from rubber waste was successfully purified. By utilizing this skim latex, most ofthe environmental problems associated with rubber industry could be ruled out and at the same time may turn it into a value-added product. Purification step is crucial in order to minimize time, energy, and cost. 2009 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/22564/1/Development_of_Patatin-like_Protein_Purification_Method.pdf Abdullah, Nurul Ain Harmiza and Yusof, Faridah and Mohd Nor, Zairossani (2009) Development of patatin-like protein purification method from natural rubber industrial waste. In: Second International Conference and Workshops on Basic and Applied Sciences (2nd ICOWOBAS) & Regional Annual Fundamental Science Seminar 2009, 2 - 4 June 2009, The ZON Regency Hotel, Johor Bahru.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Abdullah, Nurul Ain Harmiza Yusof, Faridah Mohd Nor, Zairossani Development of patatin-like protein purification method from natural rubber industrial waste
description	Skim latex is the byproduct of natural rubber processing. It is treated as effluent and lavishly left in acid pond treatment which creates a lot of environmental problems. The serum extracted from skim latex was found to contain patatin-like protein which is homologous to patatin storage proteins in potato and tomato. Patatin belongs to a group of plant storage glycoproteins that show lipid acyl hydrolase activity which may be a means of defense against plant parasites. A definite method to purify patatin-like protein from rubber waste with minimal loss of target protein is therefore desirable. Three different methods of column chromatography were employed in the purification process to obtain the purified target protein. The success of this protein purification technique was assayed by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, lipid acyl hydrolase activity analysis and Bradford determination of protein concentration to quantify the protein yield. Through this method, the patatin-like protein from rubber waste was successfully purified. By utilizing this skim latex, most ofthe environmental problems associated with rubber industry could be ruled out and at the same time may turn it into a value-added product. Purification step is crucial in order to minimize time, energy, and cost.
format	Conference or Workshop Item
author	Abdullah, Nurul Ain Harmiza Yusof, Faridah Mohd Nor, Zairossani
author_facet	Abdullah, Nurul Ain Harmiza Yusof, Faridah Mohd Nor, Zairossani
author_sort	Abdullah, Nurul Ain Harmiza
title	Development of patatin-like protein purification method from natural rubber industrial waste
title_short	Development of patatin-like protein purification method from natural rubber industrial waste
title_full	Development of patatin-like protein purification method from natural rubber industrial waste
title_fullStr	Development of patatin-like protein purification method from natural rubber industrial waste
title_full_unstemmed	Development of patatin-like protein purification method from natural rubber industrial waste
title_sort	development of patatin-like protein purification method from natural rubber industrial waste
publishDate	2009
url	http://irep.iium.edu.my/22564/ http://irep.iium.edu.my/22564/1/Development_of_Patatin-like_Protein_Purification_Method.pdf
first_indexed	2023-09-18T20:34:20Z
last_indexed	2023-09-18T20:34:20Z
_version_	1777408944485957632

Development of patatin-like protein purification method from natural rubber industrial waste

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