Simulated mutagenesis to predict the mutational effect on the thermostability of Bacillus circulans xylanase
Due to the environmental concerns, the use of hemicellulolytic enzymes has recently attracted considerable interest as a substitute for chlorine chemicals in pulp bleaching. The challenges in incorporating enzyme to the bleaching system are to have a stable and active xylanase at high temperatur...
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Research Journal of Chemistry and Environment
2010
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iium-22472011-11-16T02:56:40Z http://irep.iium.edu.my/2247/ Simulated mutagenesis to predict the mutational effect on the thermostability of Bacillus circulans xylanase Abdul Hadi, Muaz Noorbatcha, Ibrahim Ali Ismail, Ahmad Faris Mohd. Salleh, Hamzah QD Chemistry TP248.13 Biotechnology Due to the environmental concerns, the use of hemicellulolytic enzymes has recently attracted considerable interest as a substitute for chlorine chemicals in pulp bleaching. The challenges in incorporating enzyme to the bleaching system are to have a stable and active xylanase at high temperatures. To date, numerous factors have been considered for putative determinants of protein thermostability. Yet, there is no universal theory or rule to explain the principles underlying protein thermostability, as thermostability appears to be interplayed by many factors. In this work we have used computational methods to analyze the structural factors responsible for the themostability of Bacillus circulans xylanase which have been identified as one of the promising xylanase source to treat the pulp before bleaching it through the conventional bleaching sequences. Simulated point mutation shows that arginine substitution potentially increased the number of hydrogen bond; correlate with the xylanase activity and some changes in amino acid sequence specifically to the α-helix and β-sheet appeared to be promising in improving thermostability of xylanase together with some changes in salt bridges and accessible surface. This in silico method results can be used to develop new efficient xylanase for pulp bleaching industry. Research Journal of Chemistry and Environment 2010-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/2247/1/Simulated_mutagenesis452_paper_195-199.pdf Abdul Hadi, Muaz and Noorbatcha, Ibrahim Ali and Ismail, Ahmad Faris and Mohd. Salleh, Hamzah (2010) Simulated mutagenesis to predict the mutational effect on the thermostability of Bacillus circulans xylanase. Research Journal of Chemistry and Environment, Specil (issue). pp. 195-199. ISSN 0972-0626 http://www.chemenviron.net |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QD Chemistry TP248.13 Biotechnology |
| spellingShingle |
QD Chemistry TP248.13 Biotechnology Abdul Hadi, Muaz Noorbatcha, Ibrahim Ali Ismail, Ahmad Faris Mohd. Salleh, Hamzah Simulated mutagenesis to predict the mutational effect on the thermostability of Bacillus circulans xylanase |
| description |
Due to the environmental concerns, the use of
hemicellulolytic enzymes has recently attracted
considerable interest as a substitute for chlorine
chemicals in pulp bleaching. The challenges in incorporating enzyme to the bleaching system are to
have a stable and active xylanase at high temperatures. To date, numerous factors have been considered for putative determinants of protein thermostability. Yet, there is no universal theory or rule to explain the principles underlying protein thermostability, as thermostability appears to be interplayed by many factors. In this work we have used computational methods to analyze the structural
factors responsible for the themostability of Bacillus
circulans xylanase which have been identified as one
of the promising xylanase source to treat the pulp
before bleaching it through the conventional bleaching sequences. Simulated point mutation shows that arginine substitution potentially increased the number of hydrogen bond; correlate with the xylanase activity and some changes in amino acid sequence specifically to the α-helix and β-sheet appeared to be promising in improving thermostability of xylanase together with some changes in salt bridges and
accessible surface. This in silico method results can
be used to develop new efficient xylanase for pulp
bleaching industry.
|
| format |
Article |
| author |
Abdul Hadi, Muaz Noorbatcha, Ibrahim Ali Ismail, Ahmad Faris Mohd. Salleh, Hamzah |
| author_facet |
Abdul Hadi, Muaz Noorbatcha, Ibrahim Ali Ismail, Ahmad Faris Mohd. Salleh, Hamzah |
| author_sort |
Abdul Hadi, Muaz |
| title |
Simulated mutagenesis to predict the mutational effect on the thermostability of Bacillus circulans xylanase |
| title_short |
Simulated mutagenesis to predict the mutational effect on the thermostability of Bacillus circulans xylanase |
| title_full |
Simulated mutagenesis to predict the mutational effect on the thermostability of Bacillus circulans xylanase |
| title_fullStr |
Simulated mutagenesis to predict the mutational effect on the thermostability of Bacillus circulans xylanase |
| title_full_unstemmed |
Simulated mutagenesis to predict the mutational effect on the thermostability of Bacillus circulans xylanase |
| title_sort |
simulated mutagenesis to predict the mutational effect on the thermostability of bacillus circulans xylanase |
| publisher |
Research Journal of Chemistry and Environment |
| publishDate |
2010 |
| url |
http://irep.iium.edu.my/2247/ http://irep.iium.edu.my/2247/ http://irep.iium.edu.my/2247/1/Simulated_mutagenesis452_paper_195-199.pdf |
| first_indexed |
2023-09-18T20:09:47Z |
| last_indexed |
2023-09-18T20:09:47Z |
| _version_ |
1777407400223965184 |