Simulated mutagenesis to predict the mutational effect on the thermostability of Bacillus circulans xylanase
Due to the environmental concerns, the use of hemicellulolytic enzymes has recently attracted considerable interest as a substitute for chlorine chemicals in pulp bleaching. The challenges in incorporating enzyme to the bleaching system are to have a stable and active xylanase at high temperatur...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Research Journal of Chemistry and Environment
2010
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/2247/ http://irep.iium.edu.my/2247/ http://irep.iium.edu.my/2247/1/Simulated_mutagenesis452_paper_195-199.pdf |
| Summary: | Due to the environmental concerns, the use of
hemicellulolytic enzymes has recently attracted
considerable interest as a substitute for chlorine
chemicals in pulp bleaching. The challenges in incorporating enzyme to the bleaching system are to
have a stable and active xylanase at high temperatures. To date, numerous factors have been considered for putative determinants of protein thermostability. Yet, there is no universal theory or rule to explain the principles underlying protein thermostability, as thermostability appears to be interplayed by many factors. In this work we have used computational methods to analyze the structural
factors responsible for the themostability of Bacillus
circulans xylanase which have been identified as one
of the promising xylanase source to treat the pulp
before bleaching it through the conventional bleaching sequences. Simulated point mutation shows that arginine substitution potentially increased the number of hydrogen bond; correlate with the xylanase activity and some changes in amino acid sequence specifically to the α-helix and β-sheet appeared to be promising in improving thermostability of xylanase together with some changes in salt bridges and
accessible surface. This in silico method results can
be used to develop new efficient xylanase for pulp
bleaching industry.
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