Isolation of 5,7-dihydroxy-8-methoxyflavone as an anti-hyperglycemic agent from the leaves of Tetracera indica MERR. (Mempelas)

The present study was carried out to scientifically prove the traditional claim of Tetracera indica Merr. which is used extensively in folk medicine as a remedy for diabetes in Malaysia. Aqueous (AQ) and methanolic (MEOH) extracts of the leaves of T. indica were prepared and experiments were conduct...

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Main Authors:	Ahmed, Qamar Uddin, Dogarai, Bashar Bello S., Latip, Jalifah, Mat So'ad, Siti Zaiton, Umar, Abdulrashid
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/22176/ http://irep.iium.edu.my/22176/ http://irep.iium.edu.my/22176/1/ICNP_2011_Conf...Abstract.jpg

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recordtype	eprints
spelling	iium-221762013-07-15T01:29:13Z http://irep.iium.edu.my/22176/ Isolation of 5,7-dihydroxy-8-methoxyflavone as an anti-hyperglycemic agent from the leaves of Tetracera indica MERR. (Mempelas) Ahmed, Qamar Uddin Dogarai, Bashar Bello S. Latip, Jalifah Mat So'ad, Siti Zaiton Umar, Abdulrashid QD Chemistry The present study was carried out to scientifically prove the traditional claim of Tetracera indica Merr. which is used extensively in folk medicine as a remedy for diabetes in Malaysia. Aqueous (AQ) and methanolic (MEOH) extracts of the leaves of T. indica were prepared and experiments were conducted in vivo using animal model. Glucose levels in male albino rats (SD strain) with hyperglycemia induced by alloxan monohydrate (160 mg/kg b.w.) were determined after the oral administration of the aqueous AQ and MEOH extracts. Two doses of each extract (250 and 500 mg/kg b.w.) were evaluated. Both extracts exhibited significant anti-hyperglycemic activity in alloxan induced diabetic rats, however in normal rats no hypoglycemic activity was observed, when compared with both +ve and –ve controlled groups. The antidiabetic activity was also found to be comparable to that of the effect produced by glibenclamide (GLBC) (0.25 mg/kg b.w.). The LD50 of both extracts was found to be more than 5000 mg/kg body weight and no lethal toxicity was observed within this range. MEOH extract of the leaves of T. indica upon repeated coloumn choromatography and recrystallization techniques afforded a major phenolic compound viz., 5,7-dihydroxy-8-methoxyflavone (1.2 gm) (Fig. 1) which revealed significant anti-hyperglycemic activity comparable to GLBC in vivo. Structure of biologically active compound was thoroughly elucidated through extensive spectroscopic (NMR, IR, UV and MASS) analysis. This study justifies the use of T. indica by the local herbalists to treat diabetes in Malaysia. 2011-11-14 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/22176/1/ICNP_2011_Conf...Abstract.jpg Ahmed, Qamar Uddin and Dogarai, Bashar Bello S. and Latip, Jalifah and Mat So'ad, Siti Zaiton and Umar, Abdulrashid (2011) Isolation of 5,7-dihydroxy-8-methoxyflavone as an anti-hyperglycemic agent from the leaves of Tetracera indica MERR. (Mempelas). In: International Conference on Natural Products (ICNP 2011): Metabolomics - A New Frontier in Natural Products Science, 14-16 November 2011, Putrajaya, Malaysia. http://www.icnp2011.upm.edu.my/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Ahmed, Qamar Uddin Dogarai, Bashar Bello S. Latip, Jalifah Mat So'ad, Siti Zaiton Umar, Abdulrashid Isolation of 5,7-dihydroxy-8-methoxyflavone as an anti-hyperglycemic agent from the leaves of Tetracera indica MERR. (Mempelas)
description	The present study was carried out to scientifically prove the traditional claim of Tetracera indica Merr. which is used extensively in folk medicine as a remedy for diabetes in Malaysia. Aqueous (AQ) and methanolic (MEOH) extracts of the leaves of T. indica were prepared and experiments were conducted in vivo using animal model. Glucose levels in male albino rats (SD strain) with hyperglycemia induced by alloxan monohydrate (160 mg/kg b.w.) were determined after the oral administration of the aqueous AQ and MEOH extracts. Two doses of each extract (250 and 500 mg/kg b.w.) were evaluated. Both extracts exhibited significant anti-hyperglycemic activity in alloxan induced diabetic rats, however in normal rats no hypoglycemic activity was observed, when compared with both +ve and –ve controlled groups. The antidiabetic activity was also found to be comparable to that of the effect produced by glibenclamide (GLBC) (0.25 mg/kg b.w.). The LD50 of both extracts was found to be more than 5000 mg/kg body weight and no lethal toxicity was observed within this range. MEOH extract of the leaves of T. indica upon repeated coloumn choromatography and recrystallization techniques afforded a major phenolic compound viz., 5,7-dihydroxy-8-methoxyflavone (1.2 gm) (Fig. 1) which revealed significant anti-hyperglycemic activity comparable to GLBC in vivo. Structure of biologically active compound was thoroughly elucidated through extensive spectroscopic (NMR, IR, UV and MASS) analysis. This study justifies the use of T. indica by the local herbalists to treat diabetes in Malaysia.
format	Conference or Workshop Item
author	Ahmed, Qamar Uddin Dogarai, Bashar Bello S. Latip, Jalifah Mat So'ad, Siti Zaiton Umar, Abdulrashid
author_facet	Ahmed, Qamar Uddin Dogarai, Bashar Bello S. Latip, Jalifah Mat So'ad, Siti Zaiton Umar, Abdulrashid
author_sort	Ahmed, Qamar Uddin
title	Isolation of 5,7-dihydroxy-8-methoxyflavone as an anti-hyperglycemic agent from the leaves of Tetracera indica MERR. (Mempelas)
title_short	Isolation of 5,7-dihydroxy-8-methoxyflavone as an anti-hyperglycemic agent from the leaves of Tetracera indica MERR. (Mempelas)
title_full	Isolation of 5,7-dihydroxy-8-methoxyflavone as an anti-hyperglycemic agent from the leaves of Tetracera indica MERR. (Mempelas)
title_fullStr	Isolation of 5,7-dihydroxy-8-methoxyflavone as an anti-hyperglycemic agent from the leaves of Tetracera indica MERR. (Mempelas)
title_full_unstemmed	Isolation of 5,7-dihydroxy-8-methoxyflavone as an anti-hyperglycemic agent from the leaves of Tetracera indica MERR. (Mempelas)
title_sort	isolation of 5,7-dihydroxy-8-methoxyflavone as an anti-hyperglycemic agent from the leaves of tetracera indica merr. (mempelas)
publishDate	2011
url	http://irep.iium.edu.my/22176/ http://irep.iium.edu.my/22176/ http://irep.iium.edu.my/22176/1/ICNP_2011_Conf...Abstract.jpg
first_indexed	2023-09-18T20:33:54Z
last_indexed	2023-09-18T20:33:54Z
_version_	1777408917798649856

Isolation of 5,7-dihydroxy-8-methoxyflavone as an anti-hyperglycemic agent from the leaves of Tetracera indica MERR. (Mempelas)

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