Screening of fungi on solid state bioconversion of oil palm empty fruit bunch for production of cellulase

Five potential strain of fungi (Trichoderma reesei RUT C-30 (ATCC 56765, USA; Trichoderma harzanarium (SCahmT 105); Trichoderma harzianum (Isolated by UPM); Penicillium 1 (isolated from sewage treatment plant);Peniczllium 2 (isolated from empty fruit bunch) were screened for production of cellula...

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Main Authors:	Abdul Karim, Mohamed Ismail, A. Hamid, Manisya Zauri, Yusof, Faridah, Alam, Md. Zahangir
Format:	Book Chapter
Language:	English
Published:	IIUM Press 2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/20983/ http://irep.iium.edu.my/20983/ http://irep.iium.edu.my/20983/1/screening_of_fungi_-_C32.pdf

id	iium-20983
recordtype	eprints
spelling	iium-209832012-07-05T01:05:26Z http://irep.iium.edu.my/20983/ Screening of fungi on solid state bioconversion of oil palm empty fruit bunch for production of cellulase Abdul Karim, Mohamed Ismail A. Hamid, Manisya Zauri Yusof, Faridah Alam, Md. Zahangir TP248.13 Biotechnology Five potential strain of fungi (Trichoderma reesei RUT C-30 (ATCC 56765, USA; Trichoderma harzanarium (SCahmT 105); Trichoderma harzianum (Isolated by UPM); Penicillium 1 (isolated from sewage treatment plant);Peniczllium 2 (isolated from empty fruit bunch) were screened for production of cellulase using solid state bioconversIOn of oil palm empty fruit bunch. Hydrolytic activities of strains were tested on carboxymethylcellulose (CMC) agar. Strain Penicillzum 2 (P 2) showed the highest radius of hydrolytic activity of 1.2 cm. This was followed by T. reesez RUT C-30 (RUT C-30) and isolated Peniczllium 1 (P 1) with radius clearance of 1.0 and 0.9 cm, respectively. These strains of culture (Ix 107 spores/ml ) were seeded in oil palm empty fruit bunch (70% moisture) supplemented with 1N of potassium dihydrogen phosphate and 1N of potassium hydrogen phosphate in shake flask experiment which was fermented for 8 days at 30°C for production of cellulase. Result obtained indicate T. reesei RUT C-30 produce the highest CMCase (1.831 U/gds) at day 6 of fermentation. For FPase Trichoderma harzianum gave high production (0.156 U/gds at 4 days fermentation. Penicillium 1 produced the highest ~-glucosidase of 2.361 U/gds at day 2fermentation. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/20983/1/screening_of_fungi_-_C32.pdf Abdul Karim, Mohamed Ismail and A. Hamid, Manisya Zauri and Yusof, Faridah and Alam, Md. Zahangir (2011) Screening of fungi on solid state bioconversion of oil palm empty fruit bunch for production of cellulase. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 251-257. ISBN 9789674181444 http://rms.research.iium.edu.my/bookstore/default.aspx
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Abdul Karim, Mohamed Ismail A. Hamid, Manisya Zauri Yusof, Faridah Alam, Md. Zahangir Screening of fungi on solid state bioconversion of oil palm empty fruit bunch for production of cellulase
description	Five potential strain of fungi (Trichoderma reesei RUT C-30 (ATCC 56765, USA; Trichoderma harzanarium (SCahmT 105); Trichoderma harzianum (Isolated by UPM); Penicillium 1 (isolated from sewage treatment plant);Peniczllium 2 (isolated from empty fruit bunch) were screened for production of cellulase using solid state bioconversIOn of oil palm empty fruit bunch. Hydrolytic activities of strains were tested on carboxymethylcellulose (CMC) agar. Strain Penicillzum 2 (P 2) showed the highest radius of hydrolytic activity of 1.2 cm. This was followed by T. reesez RUT C-30 (RUT C-30) and isolated Peniczllium 1 (P 1) with radius clearance of 1.0 and 0.9 cm, respectively. These strains of culture (Ix 107 spores/ml ) were seeded in oil palm empty fruit bunch (70% moisture) supplemented with 1N of potassium dihydrogen phosphate and 1N of potassium hydrogen phosphate in shake flask experiment which was fermented for 8 days at 30°C for production of cellulase. Result obtained indicate T. reesei RUT C-30 produce the highest CMCase (1.831 U/gds) at day 6 of fermentation. For FPase Trichoderma harzianum gave high production (0.156 U/gds at 4 days fermentation. Penicillium 1 produced the highest ~-glucosidase of 2.361 U/gds at day 2fermentation.
format	Book Chapter
author	Abdul Karim, Mohamed Ismail A. Hamid, Manisya Zauri Yusof, Faridah Alam, Md. Zahangir
author_facet	Abdul Karim, Mohamed Ismail A. Hamid, Manisya Zauri Yusof, Faridah Alam, Md. Zahangir
author_sort	Abdul Karim, Mohamed Ismail
title	Screening of fungi on solid state bioconversion of oil palm empty fruit bunch for production of cellulase
title_short	Screening of fungi on solid state bioconversion of oil palm empty fruit bunch for production of cellulase
title_full	Screening of fungi on solid state bioconversion of oil palm empty fruit bunch for production of cellulase
title_fullStr	Screening of fungi on solid state bioconversion of oil palm empty fruit bunch for production of cellulase
title_full_unstemmed	Screening of fungi on solid state bioconversion of oil palm empty fruit bunch for production of cellulase
title_sort	screening of fungi on solid state bioconversion of oil palm empty fruit bunch for production of cellulase
publisher	IIUM Press
publishDate	2011
url	http://irep.iium.edu.my/20983/ http://irep.iium.edu.my/20983/ http://irep.iium.edu.my/20983/1/screening_of_fungi_-_C32.pdf
first_indexed	2023-09-18T20:31:44Z
last_indexed	2023-09-18T20:31:44Z
_version_	1777408781018202112

Screening of fungi on solid state bioconversion of oil palm empty fruit bunch for production of cellulase

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