Design and development of a lab scale bioreactor for heat inducible enzyme expression system

Bioreactors are very important for cell growth and mass production of intended product, for example enzyme. Considerations of the process such as the level of agitation, aeration without cell damage, temperature optimization, good control environment, cell concentration, optimized medium utilizat...

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Main Authors:	Adam, Daud, Ismail, Ahmad Faris, Mohd. Salleh, Hamzah
Format:	Book Chapter
Language:	English
Published:	IIUM Press 2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/20975/ http://irep.iium.edu.my/20975/ http://irep.iium.edu.my/20975/1/design_and_development_-_C27.pdf

id	iium-20975
recordtype	eprints
spelling	iium-209752012-07-04T08:09:08Z http://irep.iium.edu.my/20975/ Design and development of a lab scale bioreactor for heat inducible enzyme expression system Adam, Daud Ismail, Ahmad Faris Mohd. Salleh, Hamzah TP248.13 Biotechnology Bioreactors are very important for cell growth and mass production of intended product, for example enzyme. Considerations of the process such as the level of agitation, aeration without cell damage, temperature optimization, good control environment, cell concentration, optimized medium utilization must be taken into consideration. Design and selection for this design project depends on cell specific demand, regulatory and economics. In order to find the suitable bioreactor for instant temperature change, heat transfer and thermodynamics concept are implemented. Thus this project aspires to design an automated bioreactor system whereby its major function is both instantaneous heating and cooling for the production of a recombinant enzyme as the induction temperature is shifted from 37°C to 55°C and returning it back to 37°C. An extra controller which has similarities to the concept of heat exchanger is applied to the design of a lab scale bioreactor. The equipment is known to be twin thermo circulator. The higher the mass flow rate of the fluid from the twin thermo circulator contributes to high heat transfer, higher efficiency, and greater temperature change. The disadvantage is the higher the mass flow rate, the greater the pressure loss and a bigger pump is needed. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/20975/1/design_and_development_-_C27.pdf Adam, Daud and Ismail, Ahmad Faris and Mohd. Salleh, Hamzah (2011) Design and development of a lab scale bioreactor for heat inducible enzyme expression system. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 211-218. ISBN 9789674181444 http://rms.research.iium.edu.my/bookstore/default.aspx
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Adam, Daud Ismail, Ahmad Faris Mohd. Salleh, Hamzah Design and development of a lab scale bioreactor for heat inducible enzyme expression system
description	Bioreactors are very important for cell growth and mass production of intended product, for example enzyme. Considerations of the process such as the level of agitation, aeration without cell damage, temperature optimization, good control environment, cell concentration, optimized medium utilization must be taken into consideration. Design and selection for this design project depends on cell specific demand, regulatory and economics. In order to find the suitable bioreactor for instant temperature change, heat transfer and thermodynamics concept are implemented. Thus this project aspires to design an automated bioreactor system whereby its major function is both instantaneous heating and cooling for the production of a recombinant enzyme as the induction temperature is shifted from 37°C to 55°C and returning it back to 37°C. An extra controller which has similarities to the concept of heat exchanger is applied to the design of a lab scale bioreactor. The equipment is known to be twin thermo circulator. The higher the mass flow rate of the fluid from the twin thermo circulator contributes to high heat transfer, higher efficiency, and greater temperature change. The disadvantage is the higher the mass flow rate, the greater the pressure loss and a bigger pump is needed.
format	Book Chapter
author	Adam, Daud Ismail, Ahmad Faris Mohd. Salleh, Hamzah
author_facet	Adam, Daud Ismail, Ahmad Faris Mohd. Salleh, Hamzah
author_sort	Adam, Daud
title	Design and development of a lab scale bioreactor for heat inducible enzyme expression system
title_short	Design and development of a lab scale bioreactor for heat inducible enzyme expression system
title_full	Design and development of a lab scale bioreactor for heat inducible enzyme expression system
title_fullStr	Design and development of a lab scale bioreactor for heat inducible enzyme expression system
title_full_unstemmed	Design and development of a lab scale bioreactor for heat inducible enzyme expression system
title_sort	design and development of a lab scale bioreactor for heat inducible enzyme expression system
publisher	IIUM Press
publishDate	2011
url	http://irep.iium.edu.my/20975/ http://irep.iium.edu.my/20975/ http://irep.iium.edu.my/20975/1/design_and_development_-_C27.pdf
first_indexed	2023-09-18T20:31:43Z
last_indexed	2023-09-18T20:31:43Z
_version_	1777408779850088448

Design and development of a lab scale bioreactor for heat inducible enzyme expression system

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