Fowl cholera vaccine production: screening and optimization of media in shake flask culture

The screening and optimization for fowl cholera vaccine production were carried out in shakes flask experiments with composition 10% of inoculums and 90% of media. The experiments were designed and analyzed using Statsoft software "STATISTICA Version 6.0". Initially seven variables were...

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Main Authors:	Mel, Maizirwan, Abdul Karim, Mohamed Ismail, Yob, Nor Jannah, Samsury, Intan Zahrah, Syed Hassan, Sharifah, Ngah Hamid, Akma
Format:	Book Chapter
Language:	English
Published:	IIUM Press 2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/20966/ http://irep.iium.edu.my/20966/1/fowl_cholera_-_C18.pdf

id	iium-20966
recordtype	eprints
spelling	iium-209662012-07-04T01:25:54Z http://irep.iium.edu.my/20966/ Fowl cholera vaccine production: screening and optimization of media in shake flask culture Mel, Maizirwan Abdul Karim, Mohamed Ismail Yob, Nor Jannah Samsury, Intan Zahrah Syed Hassan, Sharifah Ngah Hamid, Akma TP248.13 Biotechnology The screening and optimization for fowl cholera vaccine production were carried out in shakes flask experiments with composition 10% of inoculums and 90% of media. The experiments were designed and analyzed using Statsoft software "STATISTICA Version 6.0". Initially seven variables were screened using Placket Burman Design. The result of screening in shake flasks indicated that glucose, sodmm hydrogen carbonate and sodium chloride were the main variables that affected the P. multocida fermentation. These three variables were then optimized and analyzed by using Box Behnken design. The optimization and critical values of glucose, sodium hydrogen carbonate and sodium chloride were obtained which correspond to 2.651g, 5.052 g, and 5.437 g, respectively. The correlation between these three variables on protein concentration and total cell number were investigated using Response Surface Methodology. These critical concentrations of the glucose, sodium hydrogen carbonate and sodium chloride were transferred to second part of the project for scale-up fermentation in 2 liter laboratory scale fermenter. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/20966/1/fowl_cholera_-_C18.pdf Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Yob, Nor Jannah and Samsury, Intan Zahrah and Syed Hassan, Sharifah and Ngah Hamid, Akma (2011) Fowl cholera vaccine production: screening and optimization of media in shake flask culture. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 155-161. ISBN 9789674181444
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Mel, Maizirwan Abdul Karim, Mohamed Ismail Yob, Nor Jannah Samsury, Intan Zahrah Syed Hassan, Sharifah Ngah Hamid, Akma Fowl cholera vaccine production: screening and optimization of media in shake flask culture
description	The screening and optimization for fowl cholera vaccine production were carried out in shakes flask experiments with composition 10% of inoculums and 90% of media. The experiments were designed and analyzed using Statsoft software "STATISTICA Version 6.0". Initially seven variables were screened using Placket Burman Design. The result of screening in shake flasks indicated that glucose, sodmm hydrogen carbonate and sodium chloride were the main variables that affected the P. multocida fermentation. These three variables were then optimized and analyzed by using Box Behnken design. The optimization and critical values of glucose, sodium hydrogen carbonate and sodium chloride were obtained which correspond to 2.651g, 5.052 g, and 5.437 g, respectively. The correlation between these three variables on protein concentration and total cell number were investigated using Response Surface Methodology. These critical concentrations of the glucose, sodium hydrogen carbonate and sodium chloride were transferred to second part of the project for scale-up fermentation in 2 liter laboratory scale fermenter.
format	Book Chapter
author	Mel, Maizirwan Abdul Karim, Mohamed Ismail Yob, Nor Jannah Samsury, Intan Zahrah Syed Hassan, Sharifah Ngah Hamid, Akma
author_facet	Mel, Maizirwan Abdul Karim, Mohamed Ismail Yob, Nor Jannah Samsury, Intan Zahrah Syed Hassan, Sharifah Ngah Hamid, Akma
author_sort	Mel, Maizirwan
title	Fowl cholera vaccine production: screening and optimization of media in shake flask culture
title_short	Fowl cholera vaccine production: screening and optimization of media in shake flask culture
title_full	Fowl cholera vaccine production: screening and optimization of media in shake flask culture
title_fullStr	Fowl cholera vaccine production: screening and optimization of media in shake flask culture
title_full_unstemmed	Fowl cholera vaccine production: screening and optimization of media in shake flask culture
title_sort	fowl cholera vaccine production: screening and optimization of media in shake flask culture
publisher	IIUM Press
publishDate	2011
url	http://irep.iium.edu.my/20966/ http://irep.iium.edu.my/20966/1/fowl_cholera_-_C18.pdf
first_indexed	2023-09-18T20:31:41Z
last_indexed	2023-09-18T20:31:41Z
_version_	1777408778561388544

Fowl cholera vaccine production: screening and optimization of media in shake flask culture

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