Fowl cholera vaccine production: screening and optimization of media in shake flask culture
The screening and optimization for fowl cholera vaccine production were carried out in shakes flask experiments with composition 10% of inoculums and 90% of media. The experiments were designed and analyzed using Statsoft software "STATISTICA Version 6.0". Initially seven variables were...
| Main Authors: | , , , , , |
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| Format: | Book Chapter |
| Language: | English |
| Published: |
IIUM Press
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/20966/ http://irep.iium.edu.my/20966/1/fowl_cholera_-_C18.pdf |
| Summary: | The screening and optimization for fowl cholera vaccine production were carried
out in shakes flask experiments with composition 10% of inoculums and 90% of media.
The experiments were designed and analyzed using Statsoft software "STATISTICA
Version 6.0". Initially seven variables were screened using Placket Burman Design. The
result of screening in shake flasks indicated that glucose, sodmm hydrogen carbonate and
sodium chloride were the main variables that affected the P. multocida fermentation.
These three variables were then optimized and analyzed by using Box Behnken design.
The optimization and critical values of glucose, sodium hydrogen carbonate and sodium
chloride were obtained which correspond to 2.651g, 5.052 g, and 5.437 g, respectively.
The correlation between these three variables on protein concentration and total cell
number were investigated using Response Surface Methodology. These critical
concentrations of the glucose, sodium hydrogen carbonate and sodium chloride were
transferred to second part of the project for scale-up fermentation in 2 liter laboratory
scale fermenter. |
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