Investigations on spider house for anti microbial activity
The purpose of this new investigation was to determine if spider webs exhibit antibacterial properties. In order to determine antibacterial properties, the spider webs were extracted with different solvents such as methanol, ethanol, acetone, and water in different conditions (extraction time, an...
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Online Access: | http://irep.iium.edu.my/20959/ http://irep.iium.edu.my/20959/ http://irep.iium.edu.my/20959/1/investigations_on_spider_-_C14.pdf |
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iium-209592012-07-04T01:03:50Z http://irep.iium.edu.my/20959/ Investigations on spider house for anti microbial activity Mirghani, Mohamed Elwathig Saeed Zulkifli, Mohamad Zul Fahmi TP368 Food processing and manufacture The purpose of this new investigation was to determine if spider webs exhibit antibacterial properties. In order to determine antibacterial properties, the spider webs were extracted with different solvents such as methanol, ethanol, acetone, and water in different conditions (extraction time, and concentration used for optimization). These extracts were screened for antibacterial activity using disc diffusion assay. Two bacteria were used in the antibacterial assay namely Bacillus subrilis, and Escherichia coli The determination of spider webs exhibiting antibacterial properties was based, at least in this study, solely upon the definite appearance of an inhibition zone around the well of plates. In screening, acetone solvent was shown the best antibacterial activity compare to other solvents with 10 mm of diameter of inhibition zone for Bacillus subtilis and 9 mm of diameter of inhibition zone for Escherichia coli. In optimization, the maximum inhibition zone on the Bacillus subtilis was 15 rom at a time of 48 hours and concentration of 0.035 g/ml. Meanwhile, the maximum diameter of inhibition zone on the Escherichia coli was 12 mm at a time of 48 hours and concentration of 0.035 g/ml. Therefore, this study showed that spider webs could be potential source of new antibacterial agents. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/20959/1/investigations_on_spider_-_C14.pdf Mirghani, Mohamed Elwathig Saeed and Zulkifli, Mohamad Zul Fahmi (2011) Investigations on spider house for anti microbial activity. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 117-128. ISBN 9789674181444 http://rms.research.iium.edu.my/bookstore/default.aspx |
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TP368 Food processing and manufacture |
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TP368 Food processing and manufacture Mirghani, Mohamed Elwathig Saeed Zulkifli, Mohamad Zul Fahmi Investigations on spider house for anti microbial activity |
description |
The purpose of this new investigation was to determine if spider webs exhibit
antibacterial properties. In order to determine antibacterial properties, the spider webs
were extracted with different solvents such as methanol, ethanol, acetone, and water in
different conditions (extraction time, and concentration used for optimization). These
extracts were screened for antibacterial activity using disc diffusion assay. Two bacteria
were used in the antibacterial assay namely Bacillus subrilis, and Escherichia coli The
determination of spider webs exhibiting antibacterial properties was based, at least in this
study, solely upon the definite appearance of an inhibition zone around the well of plates.
In screening, acetone solvent was shown the best antibacterial activity compare to other
solvents with 10 mm of diameter of inhibition zone for Bacillus subtilis and 9 mm of
diameter of inhibition zone for Escherichia coli. In optimization, the maximum inhibition
zone on the Bacillus subtilis was 15 rom at a time of 48 hours and concentration of 0.035
g/ml. Meanwhile, the maximum diameter of inhibition zone on the Escherichia coli was
12 mm at a time of 48 hours and concentration of 0.035 g/ml. Therefore, this study
showed that spider webs could be potential source of new antibacterial agents. |
format |
Book Chapter |
author |
Mirghani, Mohamed Elwathig Saeed Zulkifli, Mohamad Zul Fahmi |
author_facet |
Mirghani, Mohamed Elwathig Saeed Zulkifli, Mohamad Zul Fahmi |
author_sort |
Mirghani, Mohamed Elwathig Saeed |
title |
Investigations on spider house for anti microbial activity |
title_short |
Investigations on spider house for anti microbial activity |
title_full |
Investigations on spider house for anti microbial activity |
title_fullStr |
Investigations on spider house for anti microbial activity |
title_full_unstemmed |
Investigations on spider house for anti microbial activity |
title_sort |
investigations on spider house for anti microbial activity |
publisher |
IIUM Press |
publishDate |
2011 |
url |
http://irep.iium.edu.my/20959/ http://irep.iium.edu.my/20959/ http://irep.iium.edu.my/20959/1/investigations_on_spider_-_C14.pdf |
first_indexed |
2023-09-18T20:31:41Z |
last_indexed |
2023-09-18T20:31:41Z |
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1777408777705750528 |