Investigations on spider house for anti microbial activity

The purpose of this new investigation was to determine if spider webs exhibit antibacterial properties. In order to determine antibacterial properties, the spider webs were extracted with different solvents such as methanol, ethanol, acetone, and water in different conditions (extraction time, an...

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Main Authors: Mirghani, Mohamed Elwathig Saeed, Zulkifli, Mohamad Zul Fahmi
Format: Book Chapter
Language:English
Published: IIUM Press 2011
Subjects:
Online Access:http://irep.iium.edu.my/20959/
http://irep.iium.edu.my/20959/
http://irep.iium.edu.my/20959/1/investigations_on_spider_-_C14.pdf
id iium-20959
recordtype eprints
spelling iium-209592012-07-04T01:03:50Z http://irep.iium.edu.my/20959/ Investigations on spider house for anti microbial activity Mirghani, Mohamed Elwathig Saeed Zulkifli, Mohamad Zul Fahmi TP368 Food processing and manufacture The purpose of this new investigation was to determine if spider webs exhibit antibacterial properties. In order to determine antibacterial properties, the spider webs were extracted with different solvents such as methanol, ethanol, acetone, and water in different conditions (extraction time, and concentration used for optimization). These extracts were screened for antibacterial activity using disc diffusion assay. Two bacteria were used in the antibacterial assay namely Bacillus subrilis, and Escherichia coli The determination of spider webs exhibiting antibacterial properties was based, at least in this study, solely upon the definite appearance of an inhibition zone around the well of plates. In screening, acetone solvent was shown the best antibacterial activity compare to other solvents with 10 mm of diameter of inhibition zone for Bacillus subtilis and 9 mm of diameter of inhibition zone for Escherichia coli. In optimization, the maximum inhibition zone on the Bacillus subtilis was 15 rom at a time of 48 hours and concentration of 0.035 g/ml. Meanwhile, the maximum diameter of inhibition zone on the Escherichia coli was 12 mm at a time of 48 hours and concentration of 0.035 g/ml. Therefore, this study showed that spider webs could be potential source of new antibacterial agents. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/20959/1/investigations_on_spider_-_C14.pdf Mirghani, Mohamed Elwathig Saeed and Zulkifli, Mohamad Zul Fahmi (2011) Investigations on spider house for anti microbial activity. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 117-128. ISBN 9789674181444 http://rms.research.iium.edu.my/bookstore/default.aspx
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP368 Food processing and manufacture
spellingShingle TP368 Food processing and manufacture
Mirghani, Mohamed Elwathig Saeed
Zulkifli, Mohamad Zul Fahmi
Investigations on spider house for anti microbial activity
description The purpose of this new investigation was to determine if spider webs exhibit antibacterial properties. In order to determine antibacterial properties, the spider webs were extracted with different solvents such as methanol, ethanol, acetone, and water in different conditions (extraction time, and concentration used for optimization). These extracts were screened for antibacterial activity using disc diffusion assay. Two bacteria were used in the antibacterial assay namely Bacillus subrilis, and Escherichia coli The determination of spider webs exhibiting antibacterial properties was based, at least in this study, solely upon the definite appearance of an inhibition zone around the well of plates. In screening, acetone solvent was shown the best antibacterial activity compare to other solvents with 10 mm of diameter of inhibition zone for Bacillus subtilis and 9 mm of diameter of inhibition zone for Escherichia coli. In optimization, the maximum inhibition zone on the Bacillus subtilis was 15 rom at a time of 48 hours and concentration of 0.035 g/ml. Meanwhile, the maximum diameter of inhibition zone on the Escherichia coli was 12 mm at a time of 48 hours and concentration of 0.035 g/ml. Therefore, this study showed that spider webs could be potential source of new antibacterial agents.
format Book Chapter
author Mirghani, Mohamed Elwathig Saeed
Zulkifli, Mohamad Zul Fahmi
author_facet Mirghani, Mohamed Elwathig Saeed
Zulkifli, Mohamad Zul Fahmi
author_sort Mirghani, Mohamed Elwathig Saeed
title Investigations on spider house for anti microbial activity
title_short Investigations on spider house for anti microbial activity
title_full Investigations on spider house for anti microbial activity
title_fullStr Investigations on spider house for anti microbial activity
title_full_unstemmed Investigations on spider house for anti microbial activity
title_sort investigations on spider house for anti microbial activity
publisher IIUM Press
publishDate 2011
url http://irep.iium.edu.my/20959/
http://irep.iium.edu.my/20959/
http://irep.iium.edu.my/20959/1/investigations_on_spider_-_C14.pdf
first_indexed 2023-09-18T20:31:41Z
last_indexed 2023-09-18T20:31:41Z
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