Batch fermentation of recombinant Escherichia coli producing β-glucuronidase using different control condition

Expenments were carried out in batch process in bioreactor to study the growth kinetics of E coli at different control condition. The process condition has been designed using Taguchi's method with three factors and two levels. From four experiment, Run 1 which has the condition of p02=40%,...

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Bibliographic Details
Main Authors: Abdul Karim, Mohamed Ismail, Mohd. Salleh, Hamzah, Mel, Maizirwan
Format: Book Chapter
Language:English
Published: IIUM Press 2011
Subjects:
Online Access:http://irep.iium.edu.my/20932/
http://irep.iium.edu.my/20932/
http://irep.iium.edu.my/20932/1/batch_fermentation_-_C8.pdf
Description
Summary:Expenments were carried out in batch process in bioreactor to study the growth kinetics of E coli at different control condition. The process condition has been designed using Taguchi's method with three factors and two levels. From four experiment, Run 1 which has the condition of p02=40%, airflow rate=0.5 vvm and pH=7.2 produce the highest product yield, Yp/s of 1.03 (g/g) and the smallest specific growth rate, u of 0.0072 h-1.