Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds

Kenaf (Hibiscus cannabinus) is composed of various active components including tannins, polyphenolics, alkaloids, essential oils and steroids. In this study, kenaf seeds oil was used as a material in observing the anticancer activity on MCF-7 cancer cell line and finding the optimum parameter to...

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Main Authors:	Amid, Azura, Jamal, Parveen, Mohamad, Nurul Elyani, Engku Abdullah, Engku Hasmah
Format:	Book Chapter
Language:	English
Published:	IIUM Press 2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/20912/ http://irep.iium.edu.my/20912/ http://irep.iium.edu.my/20912/1/optimization_of_the_extraction_-_C3.pdf

id	iium-20912
recordtype	eprints
spelling	iium-209122012-07-03T06:38:29Z http://irep.iium.edu.my/20912/ Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds Amid, Azura Jamal, Parveen Mohamad, Nurul Elyani Engku Abdullah, Engku Hasmah TP248.13 Biotechnology Kenaf (Hibiscus cannabinus) is composed of various active components including tannins, polyphenolics, alkaloids, essential oils and steroids. In this study, kenaf seeds oil was used as a material in observing the anticancer activity on MCF-7 cancer cell line and finding the optimum parameter to extract the oil that give the highest anticancer activity. Kenaf variety Khon kaen seeds were extracted using Supercritical Fluid Extractor (SFE). The extraction was done at the different level of temperature and pressure which range from 40 to 80°C and 200 to 600 bars respectively. Among these two parameters, temperature shows the highest impact on the percentage viability ofMCF-7 breast cancer cell line. All the oils were cytotoxic towards MCF-7 breast cancer cell line in dose dependent manner as detected using the SRB assay. Kenaf seeds oil extracted from SFE at temperature of 40°C and pressure of 400 bars was the most cytotoxic towards MCF-7 breast cancer cell line with the IC50 value of 321.43 ug/ml. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/20912/1/optimization_of_the_extraction_-_C3.pdf Amid, Azura and Jamal, Parveen and Mohamad, Nurul Elyani and Engku Abdullah, Engku Hasmah (2011) Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds. In: Current research and development in biotechnology engineering at IIUM. IIUM Press, Kuala Lumpur, pp. 17-23. ISBN 9789674181444 http://rms.research.iium.edu.my/bookstore/default.aspx
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Amid, Azura Jamal, Parveen Mohamad, Nurul Elyani Engku Abdullah, Engku Hasmah Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds
description	Kenaf (Hibiscus cannabinus) is composed of various active components including tannins, polyphenolics, alkaloids, essential oils and steroids. In this study, kenaf seeds oil was used as a material in observing the anticancer activity on MCF-7 cancer cell line and finding the optimum parameter to extract the oil that give the highest anticancer activity. Kenaf variety Khon kaen seeds were extracted using Supercritical Fluid Extractor (SFE). The extraction was done at the different level of temperature and pressure which range from 40 to 80°C and 200 to 600 bars respectively. Among these two parameters, temperature shows the highest impact on the percentage viability ofMCF-7 breast cancer cell line. All the oils were cytotoxic towards MCF-7 breast cancer cell line in dose dependent manner as detected using the SRB assay. Kenaf seeds oil extracted from SFE at temperature of 40°C and pressure of 400 bars was the most cytotoxic towards MCF-7 breast cancer cell line with the IC50 value of 321.43 ug/ml.
format	Book Chapter
author	Amid, Azura Jamal, Parveen Mohamad, Nurul Elyani Engku Abdullah, Engku Hasmah
author_facet	Amid, Azura Jamal, Parveen Mohamad, Nurul Elyani Engku Abdullah, Engku Hasmah
author_sort	Amid, Azura
title	Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds
title_short	Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds
title_full	Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds
title_fullStr	Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds
title_full_unstemmed	Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds
title_sort	optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds
publisher	IIUM Press
publishDate	2011
url	http://irep.iium.edu.my/20912/ http://irep.iium.edu.my/20912/ http://irep.iium.edu.my/20912/1/optimization_of_the_extraction_-_C3.pdf
first_indexed	2023-09-18T20:31:36Z
last_indexed	2023-09-18T20:31:36Z
_version_	1777408772873912320

Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds

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