Effects of cell immobilization to the phytate-degrading enzyme activity
The use of immobilized whole microhial cells and/or organelles eliminates lhc often tedious. lime consuming. and expensi\'e sleps invohed in isolation and purificatioll of imraccllular enzymes. II al>o lends 10 enhance the stability of lhe enzyme by relaining its nall.....l catalytic surroun...
| Main Authors: | , , |
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| Format: | Book Chapter |
| Language: | English |
| Published: |
IIUM Press
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/20776/ http://irep.iium.edu.my/20776/ http://irep.iium.edu.my/20776/1/EFFECTS_OF_CELL_IMMOBILIZATION_TO_THE_PHYTATE-_DEGRADING_ENZYME_ACTNITY.pdf |
| Summary: | The use of immobilized whole microhial cells and/or organelles eliminates lhc often tedious. lime consuming. and expensi\'e sleps invohed in isolation and purificatioll of imraccllular enzymes. II al>o lends 10 enhance the stability of lhe enzyme by relaining its nall.....l catalytic surroundings during immobilizalion and subsequent cominuous operalion. A baclerium isolated from
Malaysian waslewatn pnxlucing a 1l0,-e1 phyrate-degrading enzyme namely PhyFAUIAI was immobilized in alginate gel beads. PhyFAUIA 1 is a periplasmatic enzyme and highly substralespecific 10 phytale. The enzyme aClivity wa~ invesligaled upon cell immobilization. No shift in
pH oplima of phytase of immobilized cells was obsen·ed. 110wever. the optimum lcmperaturcbwas shifted 10 50°C, which was 15°C lower lhan free PhyFAUIAI enzyme. Immobilized cells were found 10 be more stable in wider range of pll and tcmpe-ramr.: compared to free cells. The
results presenled in this work show lhe potential for using immobilized cells to produce ditrerent "~nJ-inosilOl phosphates intennediales |
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