Study of antihyperglycaemic properties of selected Malaysian antidiabetic plants in cultured 3T3-L1 cells

Diabetes Mellitus is a metabolic disease occuring worldwide caused by defects in insulin secretion, insulin action, or most commonly both. Diabetes mellitus is probably the fastest growing metabolic disease in the world and as knowledge of the heterogeneous nature of the disease increases so does t...

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Main Authors:	Azahari, Nuraniza, Khattak, Muhammad Muzaffar Ali Khan, Bakhtiar, M. Taher, Ichwan, Solachuddin Jauhari Arief
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	R Medicine (General) RB Pathology
Online Access:	http://irep.iium.edu.my/19175/ http://irep.iium.edu.my/19175/ http://irep.iium.edu.my/19175/1/Study_of_antihyperglycaemic.pdf

id	iium-19175
recordtype	eprints
spelling	iium-191752019-09-10T01:08:20Z http://irep.iium.edu.my/19175/ Study of antihyperglycaemic properties of selected Malaysian antidiabetic plants in cultured 3T3-L1 cells Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Ichwan, Solachuddin Jauhari Arief R Medicine (General) RB Pathology Diabetes Mellitus is a metabolic disease occuring worldwide caused by defects in insulin secretion, insulin action, or most commonly both. Diabetes mellitus is probably the fastest growing metabolic disease in the world and as knowledge of the heterogeneous nature of the disease increases so does the need for more challenging and appropriate therapies. The aim of the research is to evaluate the antidiabetic properties of selected antidiabetic plants used in the treatment of diabetes mellitus. The selection of plants was based on traditional reputation of usefulness in treating diabetes. Water extracts of Syzgium Polyanthum, Peronema canescens, Orthosiphon Stamineus, Lagerostroemia Speciosa, Momordica Charantia, Tinospara Crispa, Archidendrau Jiringa, Cinnamomum Zeylanicum, and Andrographis Paniculata were selected. Insulin was used as a positive control. The plants were studied on the bioactive peptides (adipokines) using an in-vitro model. 3T3-L1 adipocyte cell line is selected for this study because it plays an important role in lipid storage and glucose homeostasis. The first test is to know the ability of the plants to induce preadipocyte to adipocyte cell by using the mixture of dexamethasone, 1-isobutyl-3-methylxantine and the plant extracts. Then, continue with the MTT assay to study the toxicity level in order to get appropriate dose of the extracts. After that, protein analysis is conduct to demonstrate the plant activity that mimics insulin action. Adipogenesis, adipolysis, adiponectin and leptin protein were analyzed to assess the effect of the extract on lipid synthesis and degradation in the cultured 3T3-L1 cells by using ELISA kit. The result confirms that a preadipocyte cell was differentiated to adipocyte. Preliminary result shows that A. Paniculata and L. Speciosa extracts have strong activity in inducing lipid formation. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/19175/1/Study_of_antihyperglycaemic.pdf Azahari, Nuraniza and Khattak, Muhammad Muzaffar Ali Khan and Bakhtiar, M. Taher and Ichwan, Solachuddin Jauhari Arief (2010) Study of antihyperglycaemic properties of selected Malaysian antidiabetic plants in cultured 3T3-L1 cells. In: 3rd International Conference on Advancement in Science and Technology (iCAST) 2010, 27-29 November 2010, Vistana Hotel, Kuantan, Pahang. http://www.iium.edu.my/icast/2010/index.php?option=com_content&view=article&id=14&Itemid=17
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General) RB Pathology
spellingShingle	R Medicine (General) RB Pathology Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Ichwan, Solachuddin Jauhari Arief Study of antihyperglycaemic properties of selected Malaysian antidiabetic plants in cultured 3T3-L1 cells
description	Diabetes Mellitus is a metabolic disease occuring worldwide caused by defects in insulin secretion, insulin action, or most commonly both. Diabetes mellitus is probably the fastest growing metabolic disease in the world and as knowledge of the heterogeneous nature of the disease increases so does the need for more challenging and appropriate therapies. The aim of the research is to evaluate the antidiabetic properties of selected antidiabetic plants used in the treatment of diabetes mellitus. The selection of plants was based on traditional reputation of usefulness in treating diabetes. Water extracts of Syzgium Polyanthum, Peronema canescens, Orthosiphon Stamineus, Lagerostroemia Speciosa, Momordica Charantia, Tinospara Crispa, Archidendrau Jiringa, Cinnamomum Zeylanicum, and Andrographis Paniculata were selected. Insulin was used as a positive control. The plants were studied on the bioactive peptides (adipokines) using an in-vitro model. 3T3-L1 adipocyte cell line is selected for this study because it plays an important role in lipid storage and glucose homeostasis. The first test is to know the ability of the plants to induce preadipocyte to adipocyte cell by using the mixture of dexamethasone, 1-isobutyl-3-methylxantine and the plant extracts. Then, continue with the MTT assay to study the toxicity level in order to get appropriate dose of the extracts. After that, protein analysis is conduct to demonstrate the plant activity that mimics insulin action. Adipogenesis, adipolysis, adiponectin and leptin protein were analyzed to assess the effect of the extract on lipid synthesis and degradation in the cultured 3T3-L1 cells by using ELISA kit. The result confirms that a preadipocyte cell was differentiated to adipocyte. Preliminary result shows that A. Paniculata and L. Speciosa extracts have strong activity in inducing lipid formation.
format	Conference or Workshop Item
author	Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Ichwan, Solachuddin Jauhari Arief
author_facet	Azahari, Nuraniza Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Ichwan, Solachuddin Jauhari Arief
author_sort	Azahari, Nuraniza
title	Study of antihyperglycaemic properties of selected Malaysian antidiabetic plants in cultured 3T3-L1 cells
title_short	Study of antihyperglycaemic properties of selected Malaysian antidiabetic plants in cultured 3T3-L1 cells
title_full	Study of antihyperglycaemic properties of selected Malaysian antidiabetic plants in cultured 3T3-L1 cells
title_fullStr	Study of antihyperglycaemic properties of selected Malaysian antidiabetic plants in cultured 3T3-L1 cells
title_full_unstemmed	Study of antihyperglycaemic properties of selected Malaysian antidiabetic plants in cultured 3T3-L1 cells
title_sort	study of antihyperglycaemic properties of selected malaysian antidiabetic plants in cultured 3t3-l1 cells
publishDate	2010
url	http://irep.iium.edu.my/19175/ http://irep.iium.edu.my/19175/ http://irep.iium.edu.my/19175/1/Study_of_antihyperglycaemic.pdf
first_indexed	2023-09-18T20:28:36Z
last_indexed	2023-09-18T20:28:36Z
_version_	1777408584182661120

Study of antihyperglycaemic properties of selected Malaysian antidiabetic plants in cultured 3T3-L1 cells

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