Bioassay guided isolation of antibacterial compounds from Andrographis paniculata

Problem statement: Chronic disease-causing bacteria of medical importance have developed resistance to antibiotics, hence, necessitating distinct and constant need for safe and efficient therapeutic agents. Plants are considered potent candidate for this aim. A way out of reducing antibiotic resista...

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Main Authors:	Sule, Abubakar, Ahmed, Qamar Uddin, Abd. Samah, Othman, Omar, Muhammad Nor, Mohd Hassan, Norazian, Mohd Kamal, Laina Zarisa, Yarmo, Mohd Ambar
Format:	Article
Language:	English
Published:	Science Publications 2011
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/18644/ http://irep.iium.edu.my/18644/ http://irep.iium.edu.my/18644/ http://irep.iium.edu.my/18644/1/ajassp.2011.525.534.pdf

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spelling	iium-186442012-02-16T04:15:45Z http://irep.iium.edu.my/18644/ Bioassay guided isolation of antibacterial compounds from Andrographis paniculata Sule, Abubakar Ahmed, Qamar Uddin Abd. Samah, Othman Omar, Muhammad Nor Mohd Hassan, Norazian Mohd Kamal, Laina Zarisa Yarmo, Mohd Ambar Q Science (General) Problem statement: Chronic disease-causing bacteria of medical importance have developed resistance to antibiotics, hence, necessitating distinct and constant need for safe and efficient therapeutic agents. Plants are considered potent candidate for this aim. A way out of reducing antibiotic resistance and adverse effects on host is the employment of antibiotic resistance inhibitors of plant origin. Approach: About 5 kg pulverized Andrographis paniculata whole plant was macerated with MeOH at room temperature to get 305 g freeze dried MeOH extract. The bioautography of MeOH extract using Staphylococcus aureus and Proteus mirabilis as indicator organisms revealed the presence of two potent antibacterial compounds. MeOH extract was further fractionated and purified by silica gel column chromatography which led to the isolation of a diterpene lactone and an entlabdane diterpene glycoside upon crystallization with absolute ethanol. Results: Two antibacterial compounds viz., 3-O-â-D-glucosyl-14-deoxyandrographolide and 14-deoxyandrographolide were successfully isolated and characterized. Their structures were exclusively elucidated through spectroscopic methods (UV, IR, 1H- and 13C NMR). Conclusion: A. paniculata possesses antibacterial activity and could be potential source of a new class of antibiotics that might be useful for infectious disease chemotherapy and contro Science Publications 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/18644/1/ajassp.2011.525.534.pdf Sule, Abubakar and Ahmed, Qamar Uddin and Abd. Samah, Othman and Omar, Muhammad Nor and Mohd Hassan, Norazian and Mohd Kamal, Laina Zarisa and Yarmo, Mohd Ambar (2011) Bioassay guided isolation of antibacterial compounds from Andrographis paniculata. American Journal of Applied Sciences , 8 (6). pp. 525-534. ISSN 1546-9239 http://thescipub.com/abstract/10.3844/ajassp.2011.525.534 DOI: 10.1021/jf903629j
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Sule, Abubakar Ahmed, Qamar Uddin Abd. Samah, Othman Omar, Muhammad Nor Mohd Hassan, Norazian Mohd Kamal, Laina Zarisa Yarmo, Mohd Ambar Bioassay guided isolation of antibacterial compounds from Andrographis paniculata
description	Problem statement: Chronic disease-causing bacteria of medical importance have developed resistance to antibiotics, hence, necessitating distinct and constant need for safe and efficient therapeutic agents. Plants are considered potent candidate for this aim. A way out of reducing antibiotic resistance and adverse effects on host is the employment of antibiotic resistance inhibitors of plant origin. Approach: About 5 kg pulverized Andrographis paniculata whole plant was macerated with MeOH at room temperature to get 305 g freeze dried MeOH extract. The bioautography of MeOH extract using Staphylococcus aureus and Proteus mirabilis as indicator organisms revealed the presence of two potent antibacterial compounds. MeOH extract was further fractionated and purified by silica gel column chromatography which led to the isolation of a diterpene lactone and an entlabdane diterpene glycoside upon crystallization with absolute ethanol. Results: Two antibacterial compounds viz., 3-O-â-D-glucosyl-14-deoxyandrographolide and 14-deoxyandrographolide were successfully isolated and characterized. Their structures were exclusively elucidated through spectroscopic methods (UV, IR, 1H- and 13C NMR). Conclusion: A. paniculata possesses antibacterial activity and could be potential source of a new class of antibiotics that might be useful for infectious disease chemotherapy and contro
format	Article
author	Sule, Abubakar Ahmed, Qamar Uddin Abd. Samah, Othman Omar, Muhammad Nor Mohd Hassan, Norazian Mohd Kamal, Laina Zarisa Yarmo, Mohd Ambar
author_facet	Sule, Abubakar Ahmed, Qamar Uddin Abd. Samah, Othman Omar, Muhammad Nor Mohd Hassan, Norazian Mohd Kamal, Laina Zarisa Yarmo, Mohd Ambar
author_sort	Sule, Abubakar
title	Bioassay guided isolation of antibacterial compounds from Andrographis paniculata
title_short	Bioassay guided isolation of antibacterial compounds from Andrographis paniculata
title_full	Bioassay guided isolation of antibacterial compounds from Andrographis paniculata
title_fullStr	Bioassay guided isolation of antibacterial compounds from Andrographis paniculata
title_full_unstemmed	Bioassay guided isolation of antibacterial compounds from Andrographis paniculata
title_sort	bioassay guided isolation of antibacterial compounds from andrographis paniculata
publisher	Science Publications
publishDate	2011
url	http://irep.iium.edu.my/18644/ http://irep.iium.edu.my/18644/ http://irep.iium.edu.my/18644/ http://irep.iium.edu.my/18644/1/ajassp.2011.525.534.pdf
first_indexed	2023-09-18T20:27:49Z
last_indexed	2023-09-18T20:27:49Z
_version_	1777408534832480256

Bioassay guided isolation of antibacterial compounds from Andrographis paniculata

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