Assessment of biomolecular expression and possible physiological changes during different types of slaughtering

Traditional practices of slaughtering of chicken or other domestic animals involves knife. However, depending on the ease of handling and religious guidelines, technical differences in the slaughtering procedures exist. Such as if jugular vein will be cut or not. Besides, immediately after slaughter...

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Main Authors:	Zaman, Rahela, Rahman, Mohammad Tariqur, Syed Osman Idid, Syed Zahir Idid, Mohd. Salleh, Hamzah
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/18142/ http://irep.iium.edu.my/18142/ http://irep.iium.edu.my/18142/1/Assessment_of_biomolecular.pdf

id	iium-18142
recordtype	eprints
spelling	iium-181422012-02-09T13:45:35Z http://irep.iium.edu.my/18142/ Assessment of biomolecular expression and possible physiological changes during different types of slaughtering Zaman, Rahela Rahman, Mohammad Tariqur Syed Osman Idid, Syed Zahir Idid Mohd. Salleh, Hamzah Q Science (General) Traditional practices of slaughtering of chicken or other domestic animals involves knife. However, depending on the ease of handling and religious guidelines, technical differences in the slaughtering procedures exist. Such as if jugular vein will be cut or not. Besides, immediately after slaughtering, the animals are either released (R+) or held tightly (R-) that allows or restricts the movement of the slaughtered animals respectively. To identify possible physiological and biomolecular changes during slaughtering, chicken were slaughtered using a sharp knife. Neck of the chicken were either severed (S+) or not (S-) during slaughtering and bodied were either released immediately after slaughtering (R+) or held tightly allowing restricted movement till the animals were dead (R-). Muscles and liver from slaughtered animals were analyzed to identify possible physiological and biomolecular changes during slaughtering which varies in the mentioned parameters. The expression of acetylcholinesterase (AChE) mRNA, AChe activity, and the content of Zn and Fe were analyzed. Notably acetylcholine (Ach) is a major neurotransmiter (NT) in neuromuscular junction (NMJ) of vertebrate, release of which is related to muscle movement and blood removal; but due to its very short lifespan, it is actually measured indirectly by measuring the enzyme AChe. AChe mRNA expression was analyzed using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and the enzyme activity of AChe was measured spectrophotometrically. The expression of AChe mRNA found to be low in chickens that were slaughtered without severing neck and released immediately after slaughtering, compared to those which were slaughtered by severing the neck and/or held tightly after slaughtering. However, AChe activity was found similar in all samples. As we measured using Atomic Absorption Spectrophotometry (AAS), content of Zn and Fe in muscle and liver in different samples were also found similar range. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/18142/1/Assessment_of_biomolecular.pdf Zaman, Rahela and Rahman, Mohammad Tariqur and Syed Osman Idid, Syed Zahir Idid and Mohd. Salleh, Hamzah (2010) Assessment of biomolecular expression and possible physiological changes during different types of slaughtering. In: 3rd International Conference on Advancement in Science and Technology (iCAST) 2010, 27-29 November 2010, Vistana Hotel, Kuantan, Pahang. http://www.iium.edu.my/icast/2010/index.php?option=com_content&view=article&id=14&Itemid=17
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Zaman, Rahela Rahman, Mohammad Tariqur Syed Osman Idid, Syed Zahir Idid Mohd. Salleh, Hamzah Assessment of biomolecular expression and possible physiological changes during different types of slaughtering
description	Traditional practices of slaughtering of chicken or other domestic animals involves knife. However, depending on the ease of handling and religious guidelines, technical differences in the slaughtering procedures exist. Such as if jugular vein will be cut or not. Besides, immediately after slaughtering, the animals are either released (R+) or held tightly (R-) that allows or restricts the movement of the slaughtered animals respectively. To identify possible physiological and biomolecular changes during slaughtering, chicken were slaughtered using a sharp knife. Neck of the chicken were either severed (S+) or not (S-) during slaughtering and bodied were either released immediately after slaughtering (R+) or held tightly allowing restricted movement till the animals were dead (R-). Muscles and liver from slaughtered animals were analyzed to identify possible physiological and biomolecular changes during slaughtering which varies in the mentioned parameters. The expression of acetylcholinesterase (AChE) mRNA, AChe activity, and the content of Zn and Fe were analyzed. Notably acetylcholine (Ach) is a major neurotransmiter (NT) in neuromuscular junction (NMJ) of vertebrate, release of which is related to muscle movement and blood removal; but due to its very short lifespan, it is actually measured indirectly by measuring the enzyme AChe. AChe mRNA expression was analyzed using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and the enzyme activity of AChe was measured spectrophotometrically. The expression of AChe mRNA found to be low in chickens that were slaughtered without severing neck and released immediately after slaughtering, compared to those which were slaughtered by severing the neck and/or held tightly after slaughtering. However, AChe activity was found similar in all samples. As we measured using Atomic Absorption Spectrophotometry (AAS), content of Zn and Fe in muscle and liver in different samples were also found similar range.
format	Conference or Workshop Item
author	Zaman, Rahela Rahman, Mohammad Tariqur Syed Osman Idid, Syed Zahir Idid Mohd. Salleh, Hamzah
author_facet	Zaman, Rahela Rahman, Mohammad Tariqur Syed Osman Idid, Syed Zahir Idid Mohd. Salleh, Hamzah
author_sort	Zaman, Rahela
title	Assessment of biomolecular expression and possible physiological changes during different types of slaughtering
title_short	Assessment of biomolecular expression and possible physiological changes during different types of slaughtering
title_full	Assessment of biomolecular expression and possible physiological changes during different types of slaughtering
title_fullStr	Assessment of biomolecular expression and possible physiological changes during different types of slaughtering
title_full_unstemmed	Assessment of biomolecular expression and possible physiological changes during different types of slaughtering
title_sort	assessment of biomolecular expression and possible physiological changes during different types of slaughtering
publishDate	2010
url	http://irep.iium.edu.my/18142/ http://irep.iium.edu.my/18142/ http://irep.iium.edu.my/18142/1/Assessment_of_biomolecular.pdf
first_indexed	2023-09-18T20:27:10Z
last_indexed	2023-09-18T20:27:10Z
_version_	1777408493548994560

Assessment of biomolecular expression and possible physiological changes during different types of slaughtering

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