Optimization of serine protease purification from mango (Mangifera indica Cv. Chokanan) peel in polyethylene glycol/ dextran aqueous two phase system

Optimization of serine protease purification from mango (Mangifera indica Cv. Chokanan) peel in polyethylene glycol/ dextran aqueous two phase system

Mango peel is a good source of protease but remains an industrial waste. This study focuses on the optimization of PEG/Dextran-based aqueous two-phase system ATPS) to purify serine protease from mango peel. The activity of serine protease in different phase systems was studied and then the possible...

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Main Authors: Mehrnoush, Amid, Mustafa, Shuhaimi, Sarker, Md. Zaidul Islam, Mohd Yazid, Abdul Manap
Format: Article
Language:English
English
Published: MDPI 2012
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/17978/
http://irep.iium.edu.my/17978/
http://irep.iium.edu.my/17978/
http://irep.iium.edu.my/17978/1/Intl_J_Mol_Sci.pdf
http://irep.iium.edu.my/17978/4/Optimization_of_Serine_Protease_Purification_from_Mango_%28Mangifera_indica_cv._Chokanan%29_Peel_in_Polyethylene_GlycolDextran_Aqueous_Two_Phase_System.pdf
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recordtype eprints
spelling iium-179782016-12-15T07:22:53Z http://irep.iium.edu.my/17978/ Optimization of serine protease purification from mango (Mangifera indica Cv. Chokanan) peel in polyethylene glycol/ dextran aqueous two phase system Mehrnoush, Amid Mustafa, Shuhaimi Sarker, Md. Zaidul Islam Mohd Yazid, Abdul Manap QD Chemistry Mango peel is a good source of protease but remains an industrial waste. This study focuses on the optimization of PEG/Dextran-based aqueous two-phase system ATPS) to purify serine protease from mango peel. The activity of serine protease in different phase systems was studied and then the possible relationship between the purification variables, namely polyethylene glycol molecular weight (PEG, 4000-12,000 g mol-1), tie line length (-3.42-35.27%), NaCl (-2.5-11.5%) and pH (4.5-10.5) on the enzymatic properties of purified enzyme was investigated. The most significant effect of PEG was on the efficiency of serine protease purification. Also, there was a significant increase in the partition coefficient with the addition of 4.5% of NaCl to the system. This could be due to the high hydrophobicity of serine protease compared to protein contaminates. The optimum conditions to achieve high partition coefficient (84.2) purification factor (14.37) and yield (97.3%) of serine protease were obtained in the presence of 8000 g mol-1 of PEG, 17.2% of tie line length and 4.5% of NaCl at pH 7.5. The enzymatic properties of purified serine protease using PEG/dextran ATPS showed that the enzyme could be purified at a high level of purification factor and yield with easy scale-up and fast processing. MDPI 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/17978/1/Intl_J_Mol_Sci.pdf application/pdf en http://irep.iium.edu.my/17978/4/Optimization_of_Serine_Protease_Purification_from_Mango_%28Mangifera_indica_cv._Chokanan%29_Peel_in_Polyethylene_GlycolDextran_Aqueous_Two_Phase_System.pdf Mehrnoush, Amid and Mustafa, Shuhaimi and Sarker, Md. Zaidul Islam and Mohd Yazid, Abdul Manap (2012) Optimization of serine protease purification from mango (Mangifera indica Cv. Chokanan) peel in polyethylene glycol/ dextran aqueous two phase system. International Journal of Molecular Sciences, 13. pp. 3636-3649. ISSN 1422-0067 http://www.mdpi.com/journal/ijms doi:10.3390/ijms13033636
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic QD Chemistry
spellingShingle QD Chemistry
Mehrnoush, Amid
Mustafa, Shuhaimi
Sarker, Md. Zaidul Islam
Mohd Yazid, Abdul Manap
Optimization of serine protease purification from mango (Mangifera indica Cv. Chokanan) peel in polyethylene glycol/ dextran aqueous two phase system
description Mango peel is a good source of protease but remains an industrial waste. This study focuses on the optimization of PEG/Dextran-based aqueous two-phase system ATPS) to purify serine protease from mango peel. The activity of serine protease in different phase systems was studied and then the possible relationship between the purification variables, namely polyethylene glycol molecular weight (PEG, 4000-12,000 g mol-1), tie line length (-3.42-35.27%), NaCl (-2.5-11.5%) and pH (4.5-10.5) on the enzymatic properties of purified enzyme was investigated. The most significant effect of PEG was on the efficiency of serine protease purification. Also, there was a significant increase in the partition coefficient with the addition of 4.5% of NaCl to the system. This could be due to the high hydrophobicity of serine protease compared to protein contaminates. The optimum conditions to achieve high partition coefficient (84.2) purification factor (14.37) and yield (97.3%) of serine protease were obtained in the presence of 8000 g mol-1 of PEG, 17.2% of tie line length and 4.5% of NaCl at pH 7.5. The enzymatic properties of purified serine protease using PEG/dextran ATPS showed that the enzyme could be purified at a high level of purification factor and yield with easy scale-up and fast processing.
format Article
author Mehrnoush, Amid
Mustafa, Shuhaimi
Sarker, Md. Zaidul Islam
Mohd Yazid, Abdul Manap
author_facet Mehrnoush, Amid
Mustafa, Shuhaimi
Sarker, Md. Zaidul Islam
Mohd Yazid, Abdul Manap
author_sort Mehrnoush, Amid
title Optimization of serine protease purification from mango (Mangifera indica Cv. Chokanan) peel in polyethylene glycol/ dextran aqueous two phase system
title_short Optimization of serine protease purification from mango (Mangifera indica Cv. Chokanan) peel in polyethylene glycol/ dextran aqueous two phase system
title_full Optimization of serine protease purification from mango (Mangifera indica Cv. Chokanan) peel in polyethylene glycol/ dextran aqueous two phase system
title_fullStr Optimization of serine protease purification from mango (Mangifera indica Cv. Chokanan) peel in polyethylene glycol/ dextran aqueous two phase system
title_full_unstemmed Optimization of serine protease purification from mango (Mangifera indica Cv. Chokanan) peel in polyethylene glycol/ dextran aqueous two phase system
title_sort optimization of serine protease purification from mango (mangifera indica cv. chokanan) peel in polyethylene glycol/ dextran aqueous two phase system
publisher MDPI
publishDate 2012
url http://irep.iium.edu.my/17978/
http://irep.iium.edu.my/17978/
http://irep.iium.edu.my/17978/
http://irep.iium.edu.my/17978/1/Intl_J_Mol_Sci.pdf
http://irep.iium.edu.my/17978/4/Optimization_of_Serine_Protease_Purification_from_Mango_%28Mangifera_indica_cv._Chokanan%29_Peel_in_Polyethylene_GlycolDextran_Aqueous_Two_Phase_System.pdf
first_indexed 2023-09-18T20:27:01Z
last_indexed 2023-09-18T20:27:01Z
_version_ 1777408484283777024

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