Optimization of the conditions for extraction of serine protease from Kesinai plant (Streblus asper) leaves using Response Surface Methodology

Optimization of the conditions for extraction of serine protease from Kesinai plant (Streblus asper) leaves using Response Surface Methodology

Response surface methodology (RSM) using a central composite design (CCD) was employed to optimize the conditions for extraction of serine protease from kesinai (Streblus asper) leaves. The effect of independent variables, namely temperature (42.5,47.5, X1), mixing time (2–6 min, X2), buffer cont...

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Main Authors: Mehrnoush, Amid, Mustafa, Shuhaimi, Sarker, Md. Zaidul Islam, Mohd. Yazid, Abdul Manap
Format: Article
Language:English
Published: M D P I AG 2011
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/17924/
http://irep.iium.edu.my/17924/
http://irep.iium.edu.my/17924/
http://irep.iium.edu.my/17924/2/Molecules-2011a.pdf
id iium-17924
recordtype eprints
spelling iium-179242013-06-25T03:46:51Z http://irep.iium.edu.my/17924/ Optimization of the conditions for extraction of serine protease from Kesinai plant (Streblus asper) leaves using Response Surface Methodology Mehrnoush, Amid Mustafa, Shuhaimi Sarker, Md. Zaidul Islam Mohd. Yazid, Abdul Manap QD Chemistry Response surface methodology (RSM) using a central composite design (CCD) was employed to optimize the conditions for extraction of serine protease from kesinai (Streblus asper) leaves. The effect of independent variables, namely temperature (42.5,47.5, X1), mixing time (2–6 min, X2), buffer content (0–80 mL, X3) and buffer pH (4.5–10.5, X4) on specific activity, storage stability, temperature and oxidizing agent stability of serine protease from kesinai leaves was investigated. The study demonstrated that use of the optimum temperature, mixing time, buffer content and buffer pH conditions protected serine protease during extraction, as demonstrated by low activity loss. It was found that the interaction effect of mixing time and buffer content improved the serine protease stability, and the buffer pH had the most significant effect on the specific activity of the enzyme. The most desirable conditions of 2.5 °C temperature, 4 min mixing time, 40 mL buffer at pH 7.5 was established for serine protease extraction from kesinai leaves. M D P I AG 2011-11-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/17924/2/Molecules-2011a.pdf Mehrnoush, Amid and Mustafa, Shuhaimi and Sarker, Md. Zaidul Islam and Mohd. Yazid, Abdul Manap (2011) Optimization of the conditions for extraction of serine protease from Kesinai plant (Streblus asper) leaves using Response Surface Methodology. Molecules , 16 (11). pp. 9245-9260. ISSN 1420-3049 http://www.mdpi.com/1420-3049/16/11/9245/ doi:10.3390/molecules16119245
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Mehrnoush, Amid
Mustafa, Shuhaimi
Sarker, Md. Zaidul Islam
Mohd. Yazid, Abdul Manap
Optimization of the conditions for extraction of serine protease from Kesinai plant (Streblus asper) leaves using Response Surface Methodology
description Response surface methodology (RSM) using a central composite design (CCD) was employed to optimize the conditions for extraction of serine protease from kesinai (Streblus asper) leaves. The effect of independent variables, namely temperature (42.5,47.5, X1), mixing time (2–6 min, X2), buffer content (0–80 mL, X3) and buffer pH (4.5–10.5, X4) on specific activity, storage stability, temperature and oxidizing agent stability of serine protease from kesinai leaves was investigated. The study demonstrated that use of the optimum temperature, mixing time, buffer content and buffer pH conditions protected serine protease during extraction, as demonstrated by low activity loss. It was found that the interaction effect of mixing time and buffer content improved the serine protease stability, and the buffer pH had the most significant effect on the specific activity of the enzyme. The most desirable conditions of 2.5 °C temperature, 4 min mixing time, 40 mL buffer at pH 7.5 was established for serine protease extraction from kesinai leaves.
format Article
author Mehrnoush, Amid
Mustafa, Shuhaimi
Sarker, Md. Zaidul Islam
Mohd. Yazid, Abdul Manap
author_facet Mehrnoush, Amid
Mustafa, Shuhaimi
Sarker, Md. Zaidul Islam
Mohd. Yazid, Abdul Manap
author_sort Mehrnoush, Amid
title Optimization of the conditions for extraction of serine protease from Kesinai plant (Streblus asper) leaves using Response Surface Methodology
title_short Optimization of the conditions for extraction of serine protease from Kesinai plant (Streblus asper) leaves using Response Surface Methodology
title_full Optimization of the conditions for extraction of serine protease from Kesinai plant (Streblus asper) leaves using Response Surface Methodology
title_fullStr Optimization of the conditions for extraction of serine protease from Kesinai plant (Streblus asper) leaves using Response Surface Methodology
title_full_unstemmed Optimization of the conditions for extraction of serine protease from Kesinai plant (Streblus asper) leaves using Response Surface Methodology
title_sort optimization of the conditions for extraction of serine protease from kesinai plant (streblus asper) leaves using response surface methodology
publisher M D P I AG
publishDate 2011
url http://irep.iium.edu.my/17924/
http://irep.iium.edu.my/17924/
http://irep.iium.edu.my/17924/
http://irep.iium.edu.my/17924/2/Molecules-2011a.pdf
first_indexed 2023-09-18T20:26:58Z
last_indexed 2023-09-18T20:26:58Z
_version_ 1777408481492467712

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