Purification of serine protease from mango (Mangifera Indica Cv. Chokanan)peel using an alcohol/salt aqueous two phase system

Purification of serine protease from mango (Mangifera Indica Cv. Chokanan)peel using an alcohol/salt aqueous two phase system

An alcohol/salt-based aqueous two-phase (ATPS) system, as a novel method of purification, was employed to purify serine proteases from mango (Mangifera Indica Cv. Chokanan) peel. The effectiveness of different parameters, such as type and concentration of alcohol (1-propanol, 2-propanol, and ethanol...

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Main Authors: Sarker, Md. Zaidul Islam, Amid, Mehrnoush, Shuhaimi, Mustafa, Abdul Manap, Mohd Yazid
Format: Article
Language:English
Published: Elsevier 2012
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/17913/
http://irep.iium.edu.my/17913/
http://irep.iium.edu.my/17913/
http://irep.iium.edu.my/17913/1/1-s2.0-S0308814611017298-main.pdf
id iium-17913
recordtype eprints
spelling iium-179132016-02-24T08:15:33Z http://irep.iium.edu.my/17913/ Purification of serine protease from mango (Mangifera Indica Cv. Chokanan)peel using an alcohol/salt aqueous two phase system Sarker, Md. Zaidul Islam Amid, Mehrnoush Shuhaimi, Mustafa Abdul Manap, Mohd Yazid QD Chemistry An alcohol/salt-based aqueous two-phase (ATPS) system, as a novel method of purification, was employed to purify serine proteases from mango (Mangifera Indica Cv. Chokanan) peel. The effectiveness of different parameters, such as type and concentration of alcohol (1-propanol, 2-propanol, and ethanol), type of salt (sodium citrate, potassium phosphate, and ammonium sulphate), pH, and NaCl, on the purification and selective separation of serine protease was investigated. Desirable conditions of partition coefficient (K), selectivity (S), purification factor (P), and yield (Y%) of serine protease, using ATPS, were determined. The highest partition coefficient (64.5) and selectivity (343.2) for serine protease purification value were achieved in an ATPS of 16% (w/w) 2-propanaol, 19% (w/w) potassium phosphate, and 5% (w/v) NaCl at pH 7.5. It was demonstrated that serine protease could be recovered with a yield of 96.7% and a purification factor of 11.6. Elsevier 2012-11-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/17913/1/1-s2.0-S0308814611017298-main.pdf Sarker, Md. Zaidul Islam and Amid, Mehrnoush and Shuhaimi, Mustafa and Abdul Manap, Mohd Yazid (2012) Purification of serine protease from mango (Mangifera Indica Cv. Chokanan)peel using an alcohol/salt aqueous two phase system. Food Chemistry, 132 (3). pp. 1382-1386. ISSN 0308-8146 (In Press) http://www.sciencedirect.com/science/journal/03088146 10.1016/j.foodchem.2011.11.125
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Sarker, Md. Zaidul Islam
Amid, Mehrnoush
Shuhaimi, Mustafa
Abdul Manap, Mohd Yazid
Purification of serine protease from mango (Mangifera Indica Cv. Chokanan)peel using an alcohol/salt aqueous two phase system
description An alcohol/salt-based aqueous two-phase (ATPS) system, as a novel method of purification, was employed to purify serine proteases from mango (Mangifera Indica Cv. Chokanan) peel. The effectiveness of different parameters, such as type and concentration of alcohol (1-propanol, 2-propanol, and ethanol), type of salt (sodium citrate, potassium phosphate, and ammonium sulphate), pH, and NaCl, on the purification and selective separation of serine protease was investigated. Desirable conditions of partition coefficient (K), selectivity (S), purification factor (P), and yield (Y%) of serine protease, using ATPS, were determined. The highest partition coefficient (64.5) and selectivity (343.2) for serine protease purification value were achieved in an ATPS of 16% (w/w) 2-propanaol, 19% (w/w) potassium phosphate, and 5% (w/v) NaCl at pH 7.5. It was demonstrated that serine protease could be recovered with a yield of 96.7% and a purification factor of 11.6.
format Article
author Sarker, Md. Zaidul Islam
Amid, Mehrnoush
Shuhaimi, Mustafa
Abdul Manap, Mohd Yazid
author_facet Sarker, Md. Zaidul Islam
Amid, Mehrnoush
Shuhaimi, Mustafa
Abdul Manap, Mohd Yazid
author_sort Sarker, Md. Zaidul Islam
title Purification of serine protease from mango (Mangifera Indica Cv. Chokanan)peel using an alcohol/salt aqueous two phase system
title_short Purification of serine protease from mango (Mangifera Indica Cv. Chokanan)peel using an alcohol/salt aqueous two phase system
title_full Purification of serine protease from mango (Mangifera Indica Cv. Chokanan)peel using an alcohol/salt aqueous two phase system
title_fullStr Purification of serine protease from mango (Mangifera Indica Cv. Chokanan)peel using an alcohol/salt aqueous two phase system
title_full_unstemmed Purification of serine protease from mango (Mangifera Indica Cv. Chokanan)peel using an alcohol/salt aqueous two phase system
title_sort purification of serine protease from mango (mangifera indica cv. chokanan)peel using an alcohol/salt aqueous two phase system
publisher Elsevier
publishDate 2012
url http://irep.iium.edu.my/17913/
http://irep.iium.edu.my/17913/
http://irep.iium.edu.my/17913/
http://irep.iium.edu.my/17913/1/1-s2.0-S0308814611017298-main.pdf
first_indexed 2023-09-18T20:26:57Z
last_indexed 2023-09-18T20:26:57Z
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