Secondary metabolites and bioactivity studies of Garcinia griffithii plant

Nowadays, researchers are focusing their attention towards folk medicine for developing better drugs against microbial infections. Garcinia griffithii was assessed for active principles. The aim of this study is to isolate the active antimicrobial compounds from Garcinia griffithii plant. The plant...

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Main Authors:	Kamal, Tara, Omar, Muhammad Nor, Darnis, Deny Susanti, Taher, Muhammad
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/17654/ http://irep.iium.edu.my/17654/ http://irep.iium.edu.my/17654/1/Secondary_metabolites.pdf

id	iium-17654
recordtype	eprints
spelling	iium-176542012-02-04T12:45:05Z http://irep.iium.edu.my/17654/ Secondary metabolites and bioactivity studies of Garcinia griffithii plant Kamal, Tara Omar, Muhammad Nor Darnis, Deny Susanti Taher, Muhammad TP248.13 Biotechnology Nowadays, researchers are focusing their attention towards folk medicine for developing better drugs against microbial infections. Garcinia griffithii was assessed for active principles. The aim of this study is to isolate the active antimicrobial compounds from Garcinia griffithii plant. The plant materials were extracted using solvents with different polarities. The solvent extracts were then tested for antimicrobial activities based on bio-assay guided fractionation technique. It was found that the polar extracts, i.e. methanol and ethyl acetate, showed higher antimicrobial activities than the non polar (hexane) extract. The results of screening test for phytochmicals showed that the leave extract of the plant possessed the active principles e.g. terpenoids, tannins,alkaloids saponins and glycosides. The antimicrobial activity of the extracts was assayed against four bacteria and two fungal strains using the agar diffusion method by indicating the presence of the clear inhibition zones around each disc (Murray et al., 1999). Methanolic extract showed the highest inhibition zone of 18mm against S. aureus compared with Ethylacetast and Hexane extracts the bioactive compounds in methanol fraction was isolated by column chromatography on silica gel and eluted with chloroform: methanol (8.2). The minimum inhibitory concentration (MIC) and the minimum bacteria concentration (MBC) of the methanol isolated compound/s ranged from 0.013to 30mg/ml using 96 well diffusion methods. (Vollekova etal.2001 and Usman et al.,2007). MIC value for compound (1) 0.123mg/ml no MBC, MIC value for compound (2) 1.1mg/ml and MBC=MIC. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/17654/1/Secondary_metabolites.pdf Kamal, Tara and Omar, Muhammad Nor and Darnis, Deny Susanti and Taher, Muhammad (2010) Secondary metabolites and bioactivity studies of Garcinia griffithii plant. In: 3rd International Conference on Advancement in Science and Technology (iCAST) 2010, 27-29 November 2010, Vistana Hotel, Kuantan, Pahang. http://www.iium.edu.my/icast/2010/index.php?option=com_content&view=article&id=14&Itemid=17
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Kamal, Tara Omar, Muhammad Nor Darnis, Deny Susanti Taher, Muhammad Secondary metabolites and bioactivity studies of Garcinia griffithii plant
description	Nowadays, researchers are focusing their attention towards folk medicine for developing better drugs against microbial infections. Garcinia griffithii was assessed for active principles. The aim of this study is to isolate the active antimicrobial compounds from Garcinia griffithii plant. The plant materials were extracted using solvents with different polarities. The solvent extracts were then tested for antimicrobial activities based on bio-assay guided fractionation technique. It was found that the polar extracts, i.e. methanol and ethyl acetate, showed higher antimicrobial activities than the non polar (hexane) extract. The results of screening test for phytochmicals showed that the leave extract of the plant possessed the active principles e.g. terpenoids, tannins,alkaloids saponins and glycosides. The antimicrobial activity of the extracts was assayed against four bacteria and two fungal strains using the agar diffusion method by indicating the presence of the clear inhibition zones around each disc (Murray et al., 1999). Methanolic extract showed the highest inhibition zone of 18mm against S. aureus compared with Ethylacetast and Hexane extracts the bioactive compounds in methanol fraction was isolated by column chromatography on silica gel and eluted with chloroform: methanol (8.2). The minimum inhibitory concentration (MIC) and the minimum bacteria concentration (MBC) of the methanol isolated compound/s ranged from 0.013to 30mg/ml using 96 well diffusion methods. (Vollekova etal.2001 and Usman et al.,2007). MIC value for compound (1) 0.123mg/ml no MBC, MIC value for compound (2) 1.1mg/ml and MBC=MIC.
format	Conference or Workshop Item
author	Kamal, Tara Omar, Muhammad Nor Darnis, Deny Susanti Taher, Muhammad
author_facet	Kamal, Tara Omar, Muhammad Nor Darnis, Deny Susanti Taher, Muhammad
author_sort	Kamal, Tara
title	Secondary metabolites and bioactivity studies of Garcinia griffithii plant
title_short	Secondary metabolites and bioactivity studies of Garcinia griffithii plant
title_full	Secondary metabolites and bioactivity studies of Garcinia griffithii plant
title_fullStr	Secondary metabolites and bioactivity studies of Garcinia griffithii plant
title_full_unstemmed	Secondary metabolites and bioactivity studies of Garcinia griffithii plant
title_sort	secondary metabolites and bioactivity studies of garcinia griffithii plant
publishDate	2010
url	http://irep.iium.edu.my/17654/ http://irep.iium.edu.my/17654/ http://irep.iium.edu.my/17654/1/Secondary_metabolites.pdf
first_indexed	2023-09-18T20:26:38Z
last_indexed	2023-09-18T20:26:38Z
_version_	1777408460112003072

Secondary metabolites and bioactivity studies of Garcinia griffithii plant

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