Antimicrobial and antioxidant studies of Acacia mangium leaves extracts

N-hexane, acetonitrile and methanol extracts of Acacia mangium leaves were screened for their antimicrobial and antioxidant activities. Disc diffusion assay has been done on four different concentrations of those extracts. Antioxidant activities of A. mangium leaves in all three extracts were screen...

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Main Authors:	Ismail, Che Aishah Nazariah, Abd. Samah, Othman
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/17612/ http://irep.iium.edu.my/17612/ http://irep.iium.edu.my/17612/1/Antimicrobial_and_antioxidant.pdf

id	iium-17612
recordtype	eprints
spelling	iium-176122012-02-02T07:19:33Z http://irep.iium.edu.my/17612/ Antimicrobial and antioxidant studies of Acacia mangium leaves extracts Ismail, Che Aishah Nazariah Abd. Samah, Othman QR Microbiology N-hexane, acetonitrile and methanol extracts of Acacia mangium leaves were screened for their antimicrobial and antioxidant activities. Disc diffusion assay has been done on four different concentrations of those extracts. Antioxidant activities of A. mangium leaves in all three extracts were screened qualitatively by rapid Thin Layer Chromatography (TLC) antioxidant assay. Yellow spots against purple 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) background were indicated in those three extract, followed by acetonitrile and the least in n-hexane extract. Lastly, total phenolic compound (TPC) assay was performed to investigate the content of total phenols (expresses as gram of gallic acid equivalents (GAE) per 100g of extract) and also to find its correlations with antimicrobial and antioxidant activities in the plant extracts. In this diffusion assay, the result has shown that only methanol extract exhibited inhibitory action against Escherichia coli while other microorganisms (Staphylococcus aureus, Streptococcus pyogenes, Salmonella typhimurium, Escherichia coli, Cryptococcus neoformans and Candida albicans) showed resistant action against all extracts. Methanol extract at 50,100,200 and 400, mg/ml concentration has exhibited diameter zone of inhibition of 7.33+-0.58mm, 8.5+-0.500mm, 10.0+-0.00mm and 11.0+-1.00mm against E.coli respectively. It was found that the inhibitory action of methanol extract was dose-dependent with MIC of more than 20mg/ml and no MBC detected. So, its inhibitory action was found to be bacteriostatic. For total phenolic compound assay, the increase the polarity of solvent for extraction, the higher the total phenolic content was found. The finding was indicated as methanol > acetonitrile > n-hexane extracts. Higher total phenol content in methanol extract (3.56g of gallic acid equivalent (GAE) per 100g of extract) has confirmed its association with both antimicrobial and antioxidant activities in this findings. Statistical analysis also revealed that there was a significant difference in types of extracts with both antimicrobial activity (p-value = 0.022) and total phenolic content (p-value = 0.027). It can be concluded that A. mangium leaves have weak antimicrobial activities but potent antioxidant activities and possess high total phenolic compounds. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/17612/1/Antimicrobial_and_antioxidant.pdf Ismail, Che Aishah Nazariah and Abd. Samah, Othman (2010) Antimicrobial and antioxidant studies of Acacia mangium leaves extracts. In: 3rd International Conference on Advancement in Science and Technology (iCAST) 2010, 27-29 November 2010, Vistana Hotel, Kuantan, Pahang. http://www.iium.edu.my/icast/2010/index.php?option=com_content&view=article&id=14&Itemid=17
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Ismail, Che Aishah Nazariah Abd. Samah, Othman Antimicrobial and antioxidant studies of Acacia mangium leaves extracts
description	N-hexane, acetonitrile and methanol extracts of Acacia mangium leaves were screened for their antimicrobial and antioxidant activities. Disc diffusion assay has been done on four different concentrations of those extracts. Antioxidant activities of A. mangium leaves in all three extracts were screened qualitatively by rapid Thin Layer Chromatography (TLC) antioxidant assay. Yellow spots against purple 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) background were indicated in those three extract, followed by acetonitrile and the least in n-hexane extract. Lastly, total phenolic compound (TPC) assay was performed to investigate the content of total phenols (expresses as gram of gallic acid equivalents (GAE) per 100g of extract) and also to find its correlations with antimicrobial and antioxidant activities in the plant extracts. In this diffusion assay, the result has shown that only methanol extract exhibited inhibitory action against Escherichia coli while other microorganisms (Staphylococcus aureus, Streptococcus pyogenes, Salmonella typhimurium, Escherichia coli, Cryptococcus neoformans and Candida albicans) showed resistant action against all extracts. Methanol extract at 50,100,200 and 400, mg/ml concentration has exhibited diameter zone of inhibition of 7.33+-0.58mm, 8.5+-0.500mm, 10.0+-0.00mm and 11.0+-1.00mm against E.coli respectively. It was found that the inhibitory action of methanol extract was dose-dependent with MIC of more than 20mg/ml and no MBC detected. So, its inhibitory action was found to be bacteriostatic. For total phenolic compound assay, the increase the polarity of solvent for extraction, the higher the total phenolic content was found. The finding was indicated as methanol > acetonitrile > n-hexane extracts. Higher total phenol content in methanol extract (3.56g of gallic acid equivalent (GAE) per 100g of extract) has confirmed its association with both antimicrobial and antioxidant activities in this findings. Statistical analysis also revealed that there was a significant difference in types of extracts with both antimicrobial activity (p-value = 0.022) and total phenolic content (p-value = 0.027). It can be concluded that A. mangium leaves have weak antimicrobial activities but potent antioxidant activities and possess high total phenolic compounds.
format	Conference or Workshop Item
author	Ismail, Che Aishah Nazariah Abd. Samah, Othman
author_facet	Ismail, Che Aishah Nazariah Abd. Samah, Othman
author_sort	Ismail, Che Aishah Nazariah
title	Antimicrobial and antioxidant studies of Acacia mangium leaves extracts
title_short	Antimicrobial and antioxidant studies of Acacia mangium leaves extracts
title_full	Antimicrobial and antioxidant studies of Acacia mangium leaves extracts
title_fullStr	Antimicrobial and antioxidant studies of Acacia mangium leaves extracts
title_full_unstemmed	Antimicrobial and antioxidant studies of Acacia mangium leaves extracts
title_sort	antimicrobial and antioxidant studies of acacia mangium leaves extracts
publishDate	2010
url	http://irep.iium.edu.my/17612/ http://irep.iium.edu.my/17612/ http://irep.iium.edu.my/17612/1/Antimicrobial_and_antioxidant.pdf
first_indexed	2023-09-18T20:26:33Z
last_indexed	2023-09-18T20:26:33Z
_version_	1777408455474151424

Antimicrobial and antioxidant studies of Acacia mangium leaves extracts

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