Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens

Water bath stunning is a common practice in commercial slaughterhouses. Such treatment is economic and in line with animal welfare practice. However, the conditions applied for the stunning process may vary from a slaughterhouse to another slaughterhouse. Such a loose regulation on the stunning proc...

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Main Authors:	Abu Samah, Norshahida, Amid, Azura, Yusof, Faridah
Format:	Article
Language:	English
Published:	IIUM Press 2011
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/17405/ http://irep.iium.edu.my/17405/ http://irep.iium.edu.my/17405/1/over.pdf

id	iium-17405
recordtype	eprints
spelling	iium-174052012-01-31T00:32:36Z http://irep.iium.edu.my/17405/ Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens Abu Samah, Norshahida Amid, Azura Yusof, Faridah TA164 Bioengineering Water bath stunning is a common practice in commercial slaughterhouses. Such treatment is economic and in line with animal welfare practice. However, the conditions applied for the stunning process may vary from a slaughterhouse to another slaughterhouse. Such a loose regulation on the stunning procedure has opened up doors for food adulteration such as over dose stunning. In this study, a simple and reliable approach using proteomics have been developed to study the effect of different currents and voltages in stunning on the protein expression of the chickens. Protein profiles of the chickens were constructed in order to detect any differences in protein expression and modifications. The different voltage studied were 10 V, 40 V and 70 V while the values for current studied were 0.25 A, 0.5 A, and 0.75 A. After the proteomics analyses using 2D Platinum ImageMaster 6.0 and Matrix-assisted laser desorption ionization- time of flight (MALDI TOF) spectrometry identification, Voltage dependent anion channel 2 (VDAC2) was identified to be over expressed in the muscle sample of over stunned chicken. The over expression of VDAC2 was confirmed at the transcriptional level of RNA expression. Real Time PCR showed that all over stunned samples contained higher mRNA expression level for VDAC2 genes. The mRNA level of VDAC2 was upregulated by 59.87 fold change when normalized with housekeeping gene. In conclusion, VDAC2 could serve as potential biomarkers for identification of electrically stimulated chickens. The existence of these biomarkers will help to monitor the slaughtering and stunning process in the future. It will revolutionize the food authentication field and give a new breathe to the meat industry. IIUM Press 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/17405/1/over.pdf Abu Samah, Norshahida and Amid, Azura and Yusof, Faridah (2011) Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens. IIUM Engineering Journal, 12 (4). pp. 213-221. ISSN 1511-788X http://www.iium.edu.my/ejournal/index.php/iiumej/article/view/258/220
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA164 Bioengineering
spellingShingle	TA164 Bioengineering Abu Samah, Norshahida Amid, Azura Yusof, Faridah Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens
description	Water bath stunning is a common practice in commercial slaughterhouses. Such treatment is economic and in line with animal welfare practice. However, the conditions applied for the stunning process may vary from a slaughterhouse to another slaughterhouse. Such a loose regulation on the stunning procedure has opened up doors for food adulteration such as over dose stunning. In this study, a simple and reliable approach using proteomics have been developed to study the effect of different currents and voltages in stunning on the protein expression of the chickens. Protein profiles of the chickens were constructed in order to detect any differences in protein expression and modifications. The different voltage studied were 10 V, 40 V and 70 V while the values for current studied were 0.25 A, 0.5 A, and 0.75 A. After the proteomics analyses using 2D Platinum ImageMaster 6.0 and Matrix-assisted laser desorption ionization- time of flight (MALDI TOF) spectrometry identification, Voltage dependent anion channel 2 (VDAC2) was identified to be over expressed in the muscle sample of over stunned chicken. The over expression of VDAC2 was confirmed at the transcriptional level of RNA expression. Real Time PCR showed that all over stunned samples contained higher mRNA expression level for VDAC2 genes. The mRNA level of VDAC2 was upregulated by 59.87 fold change when normalized with housekeeping gene. In conclusion, VDAC2 could serve as potential biomarkers for identification of electrically stimulated chickens. The existence of these biomarkers will help to monitor the slaughtering and stunning process in the future. It will revolutionize the food authentication field and give a new breathe to the meat industry.
format	Article
author	Abu Samah, Norshahida Amid, Azura Yusof, Faridah
author_facet	Abu Samah, Norshahida Amid, Azura Yusof, Faridah
author_sort	Abu Samah, Norshahida
title	Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens
title_short	Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens
title_full	Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens
title_fullStr	Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens
title_full_unstemmed	Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens
title_sort	over expression of voltage dependent anion channel 2 (vdac2) in muscles of electrically stunned chickens
publisher	IIUM Press
publishDate	2011
url	http://irep.iium.edu.my/17405/ http://irep.iium.edu.my/17405/ http://irep.iium.edu.my/17405/1/over.pdf
first_indexed	2023-09-18T20:26:16Z
last_indexed	2023-09-18T20:26:16Z
_version_	1777408437660942336

Over expression of Voltage Dependent Anion Channel 2 (VDAC2) in muscles of electrically stunned chickens

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