Biotransfomation products from Clarius batrachus oil

Biotransformation can be defined as an application that utilized natural and recombinant organisms’ enzymes secreted by yeast, fungi and bacteria or whole cells as catalyst in synthesis of organic compound. Therefore, Pseudomonas aeruginosa bacterium has been chosen to be the biocatalyst for biotran...

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Main Authors:	Omar, Muhammad Nor, Shaban, Norhayati, Bakar, Latifah Munirah, Zuberdi, Ahmad Muzammil, Yunus, Kamaruzzaman
Format:	Conference or Workshop Item
Language:	English English
Published:	2011
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/17290/ http://irep.iium.edu.my/17290/1/IRIE_2011_Front_page_.PDF http://irep.iium.edu.my/17290/3/Biotransformation_product_catfish_oil.pdf

id	iium-17290
recordtype	eprints
spelling	iium-172902012-03-08T01:31:26Z http://irep.iium.edu.my/17290/ Biotransfomation products from Clarius batrachus oil Omar, Muhammad Nor Shaban, Norhayati Bakar, Latifah Munirah Zuberdi, Ahmad Muzammil Yunus, Kamaruzzaman Q Science (General) Biotransformation can be defined as an application that utilized natural and recombinant organisms’ enzymes secreted by yeast, fungi and bacteria or whole cells as catalyst in synthesis of organic compound. Therefore, Pseudomonas aeruginosa bacterium has been chosen to be the biocatalyst for biotransformation of ω fatty acids extracted from Malaysian catfish, Clarias batrachus emphasizing on bioconversion of arachidonic acid. In addition, arachidonic acid was one of prostaglandin precursor which exerts a variety of pharmacological effects on human and animals. In this study, the fatty acids were extracted from the catfish using modified Folch method where the fish flesh was freeze dried prior to homogenization with the chloroform and methanol system. Then, the crude lipid extract was added to the bacterial culture and incubated for 4 days. After incubation, the biotransformation product was extracted and analyzed by using gas chromatography and mass spectrometer (GC-MS) to identify the fatty acids and other compounds. It was found that several fatty acids, especially ω-fatty acids were converted to cholesterol. This indicates that ω-fatty acids can be used as starting materials for other bioactive metabolites for pharmaceutical purposes. 2011-02-09 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/17290/1/IRIE_2011_Front_page_.PDF application/pdf en http://irep.iium.edu.my/17290/3/Biotransformation_product_catfish_oil.pdf Omar, Muhammad Nor and Shaban, Norhayati and Bakar, Latifah Munirah and Zuberdi, Ahmad Muzammil and Yunus, Kamaruzzaman (2011) Biotransfomation products from Clarius batrachus oil. In: IIUM Research, Invention and Innovation Exhibition (IRIIE) 2011, 9-10 February 2011, Cultural Activity Centre (CAC) and KAED Gallery IIUM.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Omar, Muhammad Nor Shaban, Norhayati Bakar, Latifah Munirah Zuberdi, Ahmad Muzammil Yunus, Kamaruzzaman Biotransfomation products from Clarius batrachus oil
description	Biotransformation can be defined as an application that utilized natural and recombinant organisms’ enzymes secreted by yeast, fungi and bacteria or whole cells as catalyst in synthesis of organic compound. Therefore, Pseudomonas aeruginosa bacterium has been chosen to be the biocatalyst for biotransformation of ω fatty acids extracted from Malaysian catfish, Clarias batrachus emphasizing on bioconversion of arachidonic acid. In addition, arachidonic acid was one of prostaglandin precursor which exerts a variety of pharmacological effects on human and animals. In this study, the fatty acids were extracted from the catfish using modified Folch method where the fish flesh was freeze dried prior to homogenization with the chloroform and methanol system. Then, the crude lipid extract was added to the bacterial culture and incubated for 4 days. After incubation, the biotransformation product was extracted and analyzed by using gas chromatography and mass spectrometer (GC-MS) to identify the fatty acids and other compounds. It was found that several fatty acids, especially ω-fatty acids were converted to cholesterol. This indicates that ω-fatty acids can be used as starting materials for other bioactive metabolites for pharmaceutical purposes.
format	Conference or Workshop Item
author	Omar, Muhammad Nor Shaban, Norhayati Bakar, Latifah Munirah Zuberdi, Ahmad Muzammil Yunus, Kamaruzzaman
author_facet	Omar, Muhammad Nor Shaban, Norhayati Bakar, Latifah Munirah Zuberdi, Ahmad Muzammil Yunus, Kamaruzzaman
author_sort	Omar, Muhammad Nor
title	Biotransfomation products from Clarius batrachus oil
title_short	Biotransfomation products from Clarius batrachus oil
title_full	Biotransfomation products from Clarius batrachus oil
title_fullStr	Biotransfomation products from Clarius batrachus oil
title_full_unstemmed	Biotransfomation products from Clarius batrachus oil
title_sort	biotransfomation products from clarius batrachus oil
publishDate	2011
url	http://irep.iium.edu.my/17290/ http://irep.iium.edu.my/17290/1/IRIE_2011_Front_page_.PDF http://irep.iium.edu.my/17290/3/Biotransformation_product_catfish_oil.pdf
first_indexed	2023-09-18T20:26:07Z
last_indexed	2023-09-18T20:26:07Z
_version_	1777408428158746624

Biotransfomation products from Clarius batrachus oil

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