Molecular cloning of phytase gene from ASUIA279 and its expression in Pichia Pastoris system
Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZαA. The recombinant vector, pPICZαA-243HPp was then li...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
IIUM Press
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/17046/ http://irep.iium.edu.my/17046/ http://irep.iium.edu.my/17046/4/219-1318-1-PB.pdf |