An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of newcastle disease virus

The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome replication and transcription. The P either binds to the unassembled nucleocapsid (NP0) to assist viral genomic replication or binds to assembled NP (NP-RNA or NPNC) to transcribe genome to produce the sub-genomic mRN...

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Main Authors:	Ahmad Raus, Raha, Siang, Tan Wen, Yusoff, Khatijah
Format:	Conference or Workshop Item
Language:	English
Published:	2011
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/16924/ http://irep.iium.edu.my/16924/ http://irep.iium.edu.my/16924/1/An_Intermediate_In_C-Terminal.pdf

id	iium-16924
recordtype	eprints
spelling	iium-169242012-01-27T07:22:14Z http://irep.iium.edu.my/16924/ An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of newcastle disease virus Ahmad Raus, Raha Siang, Tan Wen Yusoff, Khatijah TP248.13 Biotechnology The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome replication and transcription. The P either binds to the unassembled nucleocapsid (NP0) to assist viral genomic replication or binds to assembled NP (NP-RNA or NPNC) to transcribe genome to produce the sub-genomic mRNAs. In this study, the region of P that binds to NPNC was mapped. To determine the binding region, 18 N- and C-terminally truncated P mutants were synthesized by in vitro translation in rabbit reticulocytes and mixed with purified NP (NPNC). The mutants which do not bind to NP are considered as mutants which contain deleted regions that may be involved in binding to NP. To identify which mutants that do not bind to NP, radioimmunoprecipitation and protein binding assays were used. Based on radioimmunoprecipitation analysis, it showed that the region of P that binds to NP is located within the internal region of C-terminal half of P, from amino acids 243 to279. In agreement with the radioimmunoprecipitation analysis, protein binding assay showed that the interactive domain was mapped to the internal region of the C-terminal half of P. However, a slightly bigger region of interactive domain (amino acids 224-279) was determined by the latter assay. As protein binding assay is considered more sensitive assay compared to radioimmunoprecipitation assay, thus the interactive region of P to NPNC was located within C-terminal half of P between amino acids 224 to 279. 2011-05 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/16924/1/An_Intermediate_In_C-Terminal.pdf Ahmad Raus, Raha and Siang, Tan Wen and Yusoff, Khatijah (2011) An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of newcastle disease virus. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Ahmad Raus, Raha Siang, Tan Wen Yusoff, Khatijah An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of newcastle disease virus
description	The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome replication and transcription. The P either binds to the unassembled nucleocapsid (NP0) to assist viral genomic replication or binds to assembled NP (NP-RNA or NPNC) to transcribe genome to produce the sub-genomic mRNAs. In this study, the region of P that binds to NPNC was mapped. To determine the binding region, 18 N- and C-terminally truncated P mutants were synthesized by in vitro translation in rabbit reticulocytes and mixed with purified NP (NPNC). The mutants which do not bind to NP are considered as mutants which contain deleted regions that may be involved in binding to NP. To identify which mutants that do not bind to NP, radioimmunoprecipitation and protein binding assays were used. Based on radioimmunoprecipitation analysis, it showed that the region of P that binds to NP is located within the internal region of C-terminal half of P, from amino acids 243 to279. In agreement with the radioimmunoprecipitation analysis, protein binding assay showed that the interactive domain was mapped to the internal region of the C-terminal half of P. However, a slightly bigger region of interactive domain (amino acids 224-279) was determined by the latter assay. As protein binding assay is considered more sensitive assay compared to radioimmunoprecipitation assay, thus the interactive region of P to NPNC was located within C-terminal half of P between amino acids 224 to 279.
format	Conference or Workshop Item
author	Ahmad Raus, Raha Siang, Tan Wen Yusoff, Khatijah
author_facet	Ahmad Raus, Raha Siang, Tan Wen Yusoff, Khatijah
author_sort	Ahmad Raus, Raha
title	An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of newcastle disease virus
title_short	An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of newcastle disease virus
title_full	An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of newcastle disease virus
title_fullStr	An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of newcastle disease virus
title_full_unstemmed	An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of newcastle disease virus
title_sort	intermediate region in c-terminal of phosphoprotein is required for binding to nucleocapsid of newcastle disease virus
publishDate	2011
url	http://irep.iium.edu.my/16924/ http://irep.iium.edu.my/16924/ http://irep.iium.edu.my/16924/1/An_Intermediate_In_C-Terminal.pdf
first_indexed	2023-09-18T20:25:42Z
last_indexed	2023-09-18T20:25:42Z
_version_	1777408401743020032

An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of newcastle disease virus

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